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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_20230 and AO353_20235 are separated by 201 nucleotides
AO353_20235 and AO353_20240 are separated by 97 nucleotides
AO353_20240 and AO353_20245 are separated by 111 nucleotides
AO353_20245 and AO353_20250 are separated by 472 nucleotides
AO353_20230: AO353_20230 - cytochrome B, at 4,447,866 to 4,448,423
_20230
AO353_20235: AO353_20235 - phosphonoacetaldehyde hydrolase, at 4,448,625 to 4,449,452
_20235
AO353_20240: AO353_20240 - 2-aminoethylphosphonate--pyruvate aminotransferase, at 4,449,550 to 4,450,659
_20240
AO353_20245: AO353_20245 - LysR family transcriptional regulator, at 4,450,771 to 4,451,637
_20245
AO353_20250: AO353_20250 - hypothetical protein, at 4,452,110 to 4,453,480
_20250
Group
Condition
AO353
_20230
AO353
_20235
AO353
_20240
AO353
_20245
AO353
_20250
carbon source
L-Alanine (C)
-0.2
-0.3
-0.1
-1.3
-0.2
carbon source
L-Citrulline
+0.1
-0.2
-0.5
-1.2
-0.1
carbon source
L-Leucine (C)
-0.3
-0.1
+0.1
-0.7
-0.3
nitrogen source
L-Phenylalanine (N)
-0.1
+0.0
-0.1
-0.6
-0.3
motility
outer cut, LB soft agar motility assay
-0.3
+0.0
-0.5
-0.3
+0.0
nitrogen source
Uridine (N)
-0.2
-0.1
-0.4
-0.3
+0.1
motility
outer cut, LB soft agar motility assay
+0.0
-0.8
-0.1
+0.1
-0.0
stress
methylglyoxal 0.009 vol%
-0.1
-0.3
+0.2
-0.3
-0.2
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.0
-0.2
+0.3
-0.3
-0.4
stress
Doxycycline hyclate 0.0006 mg/ml
-0.0
-0.2
+0.0
-0.8
+0.3
carbon source
L-Phenylalanine (C)
+0.2
-0.3
-0.1
-0.5
+0.2
stress
Paraquat dichloride 0.032 mg/mL
+0.1
+0.1
+0.2
-0.6
-0.3
stress
Cisplatin 0.006 mg/ml
-0.1
+0.3
+0.1
-0.8
-0.0
carbon source
L-Ornithine
+0.2
+0.0
+0.1
-0.8
+0.2
pH
Minimal media pH9
+0.0
+0.1
-0.1
-0.6
+0.3
carbon source
succinate (C)
+0.1
+0.1
-0.1
-0.6
+0.1
stress
Cisplatin 0.006 mg/ml
-0.3
+0.3
-0.3
-0.2
+0.2
stress
Ceftazidime 0.12 mg/ml
+0.1
+0.2
+0.1
-0.6
+0.1
stress
A22 0.01 mg/ml
-0.2
+0.3
+0.2
-0.4
+0.0
carbon source
L-Isoleucine (C)
-0.2
+0.4
+0.1
+0.2
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.5
+0.1
+0.0
-0.4
+0.2
denitrifying
Nitrate reduction, replicate B
+0.2
-0.4
+0.3
+0.3
+0.2
motility
outer cut, LB soft agar motility assay
+0.2
-0.1
-0.3
+0.5
+0.3
carbon source
L-Ornithine
+0.2
+0.4
-0.2
+0.3
-0.0
carbon source
L-Citrulline
+0.2
-0.1
+0.2
+0.4
-0.0
carbon source
L-Citrulline
+0.3
+0.6
+0.1
-0.2
+0.0
stress
Thallium(I) acetate 0.016 mg/ml
+0.2
+0.3
+0.3
-0.2
+0.1
radiation
Ionizing radiation, 126.196 Gy,
+0.2
+0.2
+0.1
+0.5
-0.2
carbon source
D-Glucose
+0.2
+0.6
+0.3
-0.1
-0.1
denitrifying
Nitrate reduction, replicate C
+0.2
-0.2
+0.3
+0.6
+0.2
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