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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_19945 and AO353_19950 overlap by 4 nucleotides
AO353_19950 and AO353_19955 overlap by 4 nucleotides
AO353_19955 and AO353_19960 are separated by 52 nucleotides
AO353_19960 and AO353_19965 are separated by 11 nucleotides
AO353_19945: AO353_19945 - acetyl-CoA synthetase, at 4,384,784 to 4,386,550
_19945
AO353_19950: AO353_19950 - pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha, at 4,386,547 to 4,387,530
_19950
AO353_19955: AO353_19955 - pyruvate dehydrogenase, at 4,387,527 to 4,388,507
_19955
AO353_19960: AO353_19960 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 4,388,560 to 4,389,678
_19960
AO353_19965: AO353_19965 - phosphopantetheine-binding protein, at 4,389,690 to 4,389,932
_19965
Group
Condition
AO353
_19945
AO353
_19950
AO353
_19955
AO353
_19960
AO353
_19965
stress
Doxycycline hyclate 0.0006 mg/ml
-0.0
+0.3
-0.6
-0.3
-0.9
carbon source
L-Ornithine
-0.2
-0.2
-0.1
-0.1
-0.9
carbon source
L-Alanine (C)
-0.1
-0.5
+0.0
-0.0
-0.7
nitrogen source
L-Glutamine (N)
-0.2
-0.1
+0.0
-0.5
-0.4
carbon source
L-Asparagine (C)
-0.2
-0.2
-0.2
+0.0
-0.6
motility
outer cut, LB soft agar motility assay
-0.2
-0.3
-0.0
-0.3
-0.3
stress
Lomefloxacin 0.001 mM
-0.2
+0.1
+0.1
-0.4
-0.5
carbon source
L-Citrulline
+0.2
-0.2
-0.4
-0.1
-0.4
nitrogen source
L-Threonine (N)
+0.1
-0.0
+0.1
-0.1
-0.7
stress
Benzalkonium Chloride 0.012 mg/ml
-0.1
+0.2
-0.2
+0.1
-0.5
carbon source
acetate (C)
+0.2
+0.0
-0.2
+0.0
-0.6
carbon source
L-Citrulline
+0.1
-0.1
-0.1
+0.3
-0.5
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.2
+0.2
+0.2
-0.5
+0.0
motility
outer cut, LB soft agar motility assay
-0.2
-0.4
+0.3
+0.3
-0.3
nitrogen source
nitrite (N)
-0.0
-0.2
+0.2
+0.3
-0.4
carbon source
L-Isoleucine (C)
-0.0
-0.2
-0.4
+0.1
+0.4
carbon source
L-Citrulline
+0.4
+0.0
-0.0
+0.0
-0.5
stress
1-ethyl-3-methylimidazolium chloride 170 mM
-0.2
-0.0
-0.2
+0.2
+0.4
carbon source
L-Ornithine
+0.2
-0.2
+0.3
+0.1
-0.2
carbon source
L-Leucine (C)
+0.0
-0.2
-0.3
+0.5
+0.2
stress
Cisplatin 0.006 mg/ml
+0.2
+0.0
-0.5
-0.2
+0.7
stress
Thallium(I) acetate 0.016 mg/ml
+0.2
+0.2
+0.3
-0.4
+0.0
nitrogen source
Parabanic Acid (N)
+0.2
+0.1
-0.5
+0.1
+0.4
stress
Paraquat dichloride 0.032 mg/mL
-0.1
-0.1
+0.3
-0.2
+0.5
carbon source
L-Citrulline
-0.0
+0.2
+0.3
+0.4
-0.3
carbon source
Carnitine Hydrochloride (C)
+0.1
+0.0
+0.0
-0.2
+0.6
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.0
+0.2
+0.3
-0.2
+0.3
stress
Choline Lysine 12 mM
-0.2
+0.1
-0.1
+0.3
+0.4
stress
Benzalkonium Chloride 0.01 mg/ml
-0.1
+0.1
-0.1
+0.3
+0.4
stress
Cisplatin 0.006 mg/ml
-0.1
+0.1
+0.2
+0.3
+1.2
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