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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_19250 and AO353_19255 are separated by 81 nucleotides
AO353_19255 and AO353_19260 overlap by 17 nucleotides
AO353_19260 and AO353_19265 are separated by 75 nucleotides
AO353_19265 and AO353_19270 are separated by 328 nucleotides
AO353_19250: AO353_19250 - molybdenum cofactor guanylyltransferase, at 4,221,051 to 4,221,653
_19250
AO353_19255: AO353_19255 - molybdenum cofactor biosynthesis protein B, at 4,221,735 to 4,222,274
_19255
AO353_19260: AO353_19260 - molybdenum cofactor biosynthesis protein MoaA, at 4,222,258 to 4,223,484
_19260
AO353_19265: AO353_19265 - hypothetical protein, at 4,223,560 to 4,223,754
_19265
AO353_19270: AO353_19270 - LuxR family transcriptional regulator, at 4,224,083 to 4,224,742
_19270
Group
Condition
AO353
_19250
AO353
_19255
AO353
_19260
AO353
_19265
AO353
_19270
motility
outer cut, LB soft agar motility assay
-1.9
-0.6
-0.9
-0.9
-2.3
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-2.8
-1.5
-1.2
-0.2
+0.1
motility
outer cut, LB soft agar motility assay
-0.1
-0.4
-1.3
-2.0
-1.3
motility
outer cut, LB soft agar motility assay
-0.6
-0.2
-1.0
-1.7
-0.9
stress
Rifampicin 0.005 mg/ml
-2.7
-0.4
+0.1
-0.1
-0.3
carbon source
L-Ornithine
-2.1
-0.0
-0.3
-0.3
-0.3
carbon source
L-Ornithine
-2.4
-0.0
-0.2
-0.4
+0.3
stress
Lomefloxacin 0.001 mM
-1.9
-0.2
+0.1
-0.3
-0.3
survival
4C survival; 360 hrs
-1.7
+0.0
-0.3
-0.1
-0.5
stress
copper (II) chloride 2.5 mM
-2.3
+0.3
-0.1
-0.2
+0.1
carbon source
a-Ketoglutaric (C)
-1.3
-0.0
-0.2
-0.7
+0.0
nitrogen source
L-Tryptophan (N)
-1.1
-0.2
+0.0
-0.9
-0.0
nitrogen source
nitrate (N)
-2.0
-0.1
+0.1
-0.1
-0.0
stress
Cisplatin 0.006 mg/ml
-1.7
+0.2
+0.2
-0.5
-0.2
stress
methylglyoxal 0.009 vol%
-1.5
-0.0
-0.4
-0.0
-0.1
stress
1-ethyl-3-methylimidazolium chloride 170 mM
-1.4
+0.2
-0.1
-0.4
-0.3
carbon source
L-Citrulline
-1.6
+0.0
-0.1
-0.5
+0.3
carbon source
L-Tryptophan (C)
-1.4
+0.1
+0.1
-1.0
+0.4
carbon source
L-Valine (C)
-2.0
+0.2
+0.3
-0.1
-0.3
nitrogen source
L-Serine (N)
-2.4
+0.1
+0.0
+0.3
+0.2
carbon source
L-Alanine (C)
-1.2
+0.1
+0.0
-0.8
+0.1
stress
1-ethyl-3-methylimidazolium lysine 10.5 mM
-1.3
+0.4
-0.0
-0.3
-0.3
carbon source
L-Ornithine
-1.3
+0.5
+0.0
-0.5
-0.2
carbon source
D-Ribose (C)
-1.5
+0.2
+0.2
-0.3
-0.0
stress
Nickel (II) chloride 0.625 mM
-1.0
-0.0
+0.1
-0.6
+0.2
stress
sodium fluoride 6.25 mM
-1.7
+0.3
+0.0
+0.1
+0.0
carbon source
m-Inositol (C)
-1.3
+0.0
-0.0
-0.3
+0.5
stress
Choline Lysine 12 mM
-1.3
-0.3
+0.3
+0.1
+0.2
carbon source
L-Isoleucine (C)
-0.8
+0.2
+0.3
-0.6
+0.3
carbon source
Tween 20 (C)
+1.1
-0.3
-0.1
+0.3
+0.4
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