Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_19245 and AO353_19250 are separated by 76 nucleotidesAO353_19250 and AO353_19255 are separated by 81 nucleotidesAO353_19255 and AO353_19260 overlap by 17 nucleotidesAO353_19260 and AO353_19265 are separated by 75 nucleotides AO353_19245: AO353_19245 - hypothetical protein, at 4,220,753 to 4,220,974 _19245 AO353_19250: AO353_19250 - molybdenum cofactor guanylyltransferase, at 4,221,051 to 4,221,653 _19250 AO353_19255: AO353_19255 - molybdenum cofactor biosynthesis protein B, at 4,221,735 to 4,222,274 _19255 AO353_19260: AO353_19260 - molybdenum cofactor biosynthesis protein MoaA, at 4,222,258 to 4,223,484 _19260 AO353_19265: AO353_19265 - hypothetical protein, at 4,223,560 to 4,223,754 _19265
Group Condition AO353_19245 AO353_19250 AO353_19255 AO353_19260 AO353_19265
anaerobic N1B4wt with N2E3m denitrifying replicate C N.D. -2.8 -1.5 -1.2 -0.2
motility outer cut, LB soft agar motility assay N.D. -1.9 -0.6 -0.9 -0.9
motility outer cut, LB soft agar motility assay N.D. -0.1 -0.4 -1.3 -2.0
motility outer cut, LB soft agar motility assay N.D. -0.6 -0.2 -1.0 -1.7
stress Rifampicin 0.005 mg/ml N.D. -2.7 -0.4 +0.1 -0.1
carbon source L-Ornithine N.D. -2.4 -0.0 -0.2 -0.4
carbon source L-Ornithine N.D. -2.1 -0.0 -0.3 -0.3
stress Lomefloxacin 0.001 mM N.D. -1.9 -0.2 +0.1 -0.3
stress copper (II) chloride 2.5 mM N.D. -2.3 +0.3 -0.1 -0.2
carbon source a-Ketoglutaric (C) N.D. -1.3 -0.0 -0.2 -0.7
carbon source L-Citrulline N.D. -1.6 +0.0 -0.1 -0.5
carbon source L-Tryptophan (C) N.D. -1.4 +0.1 +0.1 -1.0
nitrogen source L-Tryptophan (N) N.D. -1.1 -0.2 +0.0 -0.9
nitrogen source nitrate (N) N.D. -2.0 -0.1 +0.1 -0.1
survival 4C survival; 360 hrs N.D. -1.7 +0.0 -0.3 -0.1
nitrogen source L-Serine (N) N.D. -2.4 +0.1 +0.0 +0.3
stress Cisplatin 0.006 mg/ml N.D. -1.7 +0.2 +0.2 -0.5
stress methylglyoxal 0.009 vol% N.D. -1.5 -0.0 -0.4 -0.0
carbon source L-Alanine (C) N.D. -1.2 +0.1 +0.0 -0.8
stress 1-ethyl-3-methylimidazolium chloride 170 mM N.D. -1.4 +0.2 -0.1 -0.4
carbon source L-Valine (C) N.D. -2.0 +0.2 +0.3 -0.1
carbon source D-Ribose (C) N.D. -1.5 +0.2 +0.2 -0.3
stress A22 0.01 mg/ml N.D. -0.9 +0.2 -0.1 -0.6
carbon source L-Ornithine N.D. -1.3 +0.5 +0.0 -0.5
stress sodium fluoride 6.25 mM N.D. -1.7 +0.3 +0.0 +0.1
stress Choline Lysine 12 mM N.D. -1.3 -0.3 +0.3 +0.1
nitrogen source L-Histidine (N) N.D. -1.5 +0.1 +0.1 -0.0
stress 1-ethyl-3-methylimidazolium lysine 10.5 mM N.D. -1.3 +0.4 -0.0 -0.3
carbon source L-Isoleucine (C) N.D. -0.8 +0.2 +0.3 -0.6
anaerobic Anaerobic, acetate donor; nitrate and iron N.D. +0.9 +0.6 +0.1 +0.1
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