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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18815 and AO353_18820 are separated by 50 nucleotides
AO353_18820 and AO353_18825 are separated by 33 nucleotides
AO353_18825 and AO353_18830 are separated by 76 nucleotides
AO353_18830 and AO353_18835 are separated by 0 nucleotides
AO353_18815: AO353_18815 - flagellar motor protein MotD, at 4,137,554 to 4,138,441
_18815
AO353_18820: AO353_18820 - cobalamin biosynthesis protein CobQ, at 4,138,492 to 4,139,280
_18820
AO353_18825: AO353_18825 - chemotaxis protein CheW, at 4,139,314 to 4,140,210
_18825
AO353_18830: AO353_18830 - chemotaxis protein CheW, at 4,140,287 to 4,140,772
_18830
AO353_18835: AO353_18835 - chemotaxis protein, at 4,140,773 to 4,141,165
_18835
Group
Condition
AO353
_18815
AO353
_18820
AO353
_18825
AO353
_18830
AO353
_18835
motility
outer cut, LB soft agar motility assay
-2.7
-1.8
-2.6
-3.7
N.D.
motility
outer cut, LB soft agar motility assay
-2.1
-1.6
-2.4
-2.2
N.D.
motility
outer cut, LB soft agar motility assay
-1.8
-2.0
-1.4
-0.8
N.D.
survival
4C survival; 672 hrs
-1.4
-0.2
-1.5
-1.9
N.D.
survival
4C survival; 360 hrs
-0.9
-0.4
-0.7
-1.5
N.D.
survival
4C survival; 192 hrs
-0.7
-0.1
-0.5
-1.1
N.D.
carbon source
D-Glucose
+0.1
+0.1
-0.4
-1.2
N.D.
stress
Doxycycline hyclate 0.0006 mg/ml
+0.1
-0.4
-0.4
-0.6
N.D.
carbon source
L-Citrulline
+0.4
-0.1
-0.1
-1.4
N.D.
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.1
-0.0
-0.1
-0.9
N.D.
pH
Minimal media pH6
-0.1
+0.2
+0.2
-1.2
N.D.
pH
Minimal media pH9
-0.5
-0.4
+0.2
-0.1
N.D.
carbon source
Tween 20 (C)
-0.3
+0.1
-0.2
-0.3
N.D.
carbon source
succinate (C)
-0.2
+0.1
-0.1
-0.5
N.D.
nitrogen source
Parabanic Acid (N)
+0.2
-0.3
+0.0
-0.6
N.D.
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.7
-0.4
-0.2
-0.6
N.D.
carbon source
L-Tryptophan (C)
+0.3
+0.2
-0.4
-0.5
N.D.
stress
Thallium(I) acetate 0.016 mg/ml
+0.5
+0.1
-0.0
-0.5
N.D.
carbon source
L-Citrulline
-0.3
-0.0
-0.2
+0.5
N.D.
carbon source
L-Valine (C)
+0.5
-0.4
-0.0
+0.0
N.D.
stress
Thallium(I) acetate 0.008 mg/ml
+0.3
+0.3
-0.3
-0.2
N.D.
stress
Nickel (II) chloride 0.625 mM
+0.1
+0.1
-0.4
+0.4
N.D.
carbon source
L-Citrulline
+0.6
+0.1
-0.7
+0.4
N.D.
stress
copper (II) chloride 2.5 mM
+0.2
+0.2
-0.2
+0.4
N.D.
lb
LB
+0.3
+0.6
-0.1
-0.3
N.D.
stress
Cisplatin 0.006 mg/ml
+0.2
+0.2
-0.1
+0.3
N.D.
stress
Furfuryl Alcohol 0.25 vol%
+0.3
+0.4
+0.2
-0.1
N.D.
denitrifying
Nitrate reduction, replicate C
+0.7
-0.0
-0.1
+0.3
N.D.
radiation
Ionizing radiation, 126.196 Gy,
+0.5
-0.1
+0.2
+0.4
N.D.
stress
methylglyoxal 0.009 vol%
+0.6
+0.4
+0.2
+0.1
N.D.
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