Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18810 and AO353_18815 are separated by 12 nucleotidesAO353_18815 and AO353_18820 are separated by 50 nucleotidesAO353_18820 and AO353_18825 are separated by 33 nucleotidesAO353_18825 and AO353_18830 are separated by 76 nucleotides AO353_18810: AO353_18810 - flagellar motor protein, at 4,136,801 to 4,137,541 _18810 AO353_18815: AO353_18815 - flagellar motor protein MotD, at 4,137,554 to 4,138,441 _18815 AO353_18820: AO353_18820 - cobalamin biosynthesis protein CobQ, at 4,138,492 to 4,139,280 _18820 AO353_18825: AO353_18825 - chemotaxis protein CheW, at 4,139,314 to 4,140,210 _18825 AO353_18830: AO353_18830 - chemotaxis protein CheW, at 4,140,287 to 4,140,772 _18830
Group Condition AO353_18810 AO353_18815 AO353_18820 AO353_18825 AO353_18830
motility outer cut, LB soft agar motility assay -2.2 -2.7 -1.8 -2.6 -3.7
motility outer cut, LB soft agar motility assay -3.2 -2.1 -1.6 -2.4 -2.2
motility outer cut, LB soft agar motility assay -1.7 -1.8 -2.0 -1.4 -0.8
survival 4C survival; 672 hrs -0.8 -1.4 -0.2 -1.5 -1.9
survival 4C survival; 360 hrs -0.9 -0.9 -0.4 -0.7 -1.5
survival 4C survival; 192 hrs -0.8 -0.7 -0.1 -0.5 -1.1
stress Doxycycline hyclate 0.0006 mg/ml +0.2 +0.1 -0.4 -0.4 -0.6
carbon source D-Glucose +0.3 +0.1 +0.1 -0.4 -1.2
nitrogen source Parabanic Acid (N) -0.3 +0.2 -0.3 +0.0 -0.6
anaerobic N1B4wt with N2E3m denitrifying replicate C +0.1 -0.1 -0.0 -0.1 -0.9
pH Minimal media pH6 +0.1 -0.1 +0.2 +0.2 -1.2
pH Minimal media pH9 -0.0 -0.5 -0.4 +0.2 -0.1
carbon source L-Citrulline +0.4 +0.4 -0.1 -0.1 -1.4
carbon source L-Leucine (C) -0.5 +0.3 -0.0 +0.0 -0.5
stress acetate 68 mM -0.4 -0.4 +0.0 +0.2 -0.2
stress Benzalkonium Chloride 0.01 mg/ml -0.5 +0.2 +0.1 -0.3 -0.2
anaerobic Anaerobic, acetate donor; nitrate and iron -0.1 +0.7 -0.4 -0.2 -0.6
carbon source L-Valine (C) -0.5 +0.5 -0.4 -0.0 +0.0
nitrogen source nitrite (N) -0.4 +0.2 -0.3 -0.1 +0.2
carbon source L-Tryptophan (C) -0.1 +0.3 +0.2 -0.4 -0.5
stress Thallium(I) acetate 0.008 mg/ml -0.3 +0.4 +0.3 -0.2 -0.1
stress Thallium(I) acetate 0.008 mg/ml +0.1 +0.3 +0.3 -0.3 -0.2
carbon source L-Citrulline +0.3 -0.3 -0.0 -0.2 +0.5
lb LB -0.2 +0.3 +0.6 -0.1 -0.3
stress Nickel (II) chloride 0.625 mM +0.2 +0.1 +0.1 -0.4 +0.4
denitrifying Nitrate reduction, replicate C -0.4 +0.7 -0.0 -0.1 +0.3
stress Rifampicin 0.005 mg/ml +0.4 +0.2 -0.1 -0.3 +0.2
carbon source L-Citrulline +0.5 +0.6 +0.1 -0.7 +0.4
radiation Ionizing radiation, 126.196 Gy, +0.2 +0.5 -0.1 +0.2 +0.4
stress methylglyoxal 0.009 vol% +0.3 +0.6 +0.4 +0.2 +0.1
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