Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18795 and AO353_18800 are separated by 38 nucleotidesAO353_18800 and AO353_18805 are separated by 49 nucleotidesAO353_18805 and AO353_18810 overlap by 1 nucleotidesAO353_18810 and AO353_18815 are separated by 12 nucleotides AO353_18795: AO353_18795 - protein phosphatase, at 4,132,554 to 4,133,342 _18795 AO353_18800: AO353_18800 - chemotaxis protein CheA, at 4,133,381 to 4,135,624 _18800 AO353_18805: AO353_18805 - chemotaxis protein CheY, at 4,135,674 to 4,136,801 _18805 AO353_18810: AO353_18810 - flagellar motor protein, at 4,136,801 to 4,137,541 _18810 AO353_18815: AO353_18815 - flagellar motor protein MotD, at 4,137,554 to 4,138,441 _18815
Group Condition AO353_18795 AO353_18800 AO353_18805 AO353_18810 AO353_18815
motility outer cut, LB soft agar motility assay -1.6 -2.9 -2.2 -3.2 -2.1
motility outer cut, LB soft agar motility assay -1.0 -2.1 -3.4 -2.2 -2.7
motility outer cut, LB soft agar motility assay -1.2 -2.2 -1.7 -1.7 -1.8
survival 4C survival; 360 hrs +0.3 -1.7 -0.6 -0.9 -0.9
survival 4C survival; 672 hrs +0.5 -1.4 -0.3 -0.8 -1.4
survival 4C survival; 192 hrs +0.1 -0.9 -0.5 -0.8 -0.7
pH Minimal media pH9 -0.2 -0.6 -0.2 -0.0 -0.5
stress acetate 68 mM +0.1 -0.5 +0.1 -0.4 -0.4
carbon source L-Phenylalanine (C) -0.2 -0.2 -0.3 -0.5 +0.1
carbon source D-Serine (C) -0.2 -0.4 +0.1 -0.3 -0.2
carbon source L-Valine (C) -0.1 -0.0 -0.5 -0.5 +0.5
carbon source casaminos (C) -0.3 -0.3 +0.3 -0.6 +0.2
anaerobic N1B4wt with N2E3m denitrifying replicate C +0.4 -0.2 -0.5 +0.1 -0.1
denitrifying Nitrate reduction, replicate C -0.5 -0.0 +0.0 -0.4 +0.7
stress Benzalkonium Chloride 0.01 mg/ml +0.4 -0.2 -0.0 -0.5 +0.2
stress Choline chloride 358 mM +0.3 +0.2 -0.2 -0.6 +0.2
carbon source L-Serine (C) +0.3 +0.2 +0.4 -0.3 -0.3
carbon source L-Leucine (C) +0.2 +0.3 +0.2 -0.5 +0.3
stress acetate 34 mM +0.3 +0.6 -0.1 -0.5 +0.2
anaerobic Anaerobic, acetate donor; nitrate and iron +0.1 -0.6 +0.4 -0.1 +0.7
carbon source L-Ornithine +0.6 -0.1 -0.3 +0.3 +0.2
stress Spectinomycin 0.15 mg/ml -0.2 +0.4 +0.4 +0.3 -0.1
stress Doxycycline hyclate 0.0002 mg/ml +0.8 +0.2 -0.3 +0.2 +0.0
radiation Ionizing radiation, 126.196 Gy, +0.2 -0.2 +0.3 +0.2 +0.5
carbon source L-Citrulline +0.1 +0.2 -0.3 +0.5 +0.6
stress Cholin acetate 19 mM +0.2 +0.5 +0.4 -0.1 +0.1
stress 1-ethyl-3-methylimidazolium acetate 20 mM +0.3 +0.8 -0.0 -0.1 +0.2
carbon source L-Citrulline +0.1 +0.4 +0.1 +0.4 +0.4
stress Rifampicin 0.005 mg/ml +0.2 +0.4 +0.2 +0.4 +0.2
stress methylglyoxal 0.009 vol% +0.3 +0.2 +0.2 +0.3 +0.6
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