Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18780 and AO353_18785 overlap by 4 nucleotidesAO353_18785 and AO353_18790 are separated by 154 nucleotidesAO353_18790 and AO353_18795 are separated by 31 nucleotidesAO353_18795 and AO353_18800 are separated by 38 nucleotides AO353_18780: AO353_18780 - cobyrinic acid a,c-diamide synthase, at 4,130,417 to 4,131,247 _18780 AO353_18785: AO353_18785 - flagellar biosynthesis sigma factor, at 4,131,244 to 4,131,984 _18785 AO353_18790: AO353_18790 - histidine kinase, at 4,132,139 to 4,132,522 _18790 AO353_18795: AO353_18795 - protein phosphatase, at 4,132,554 to 4,133,342 _18795 AO353_18800: AO353_18800 - chemotaxis protein CheA, at 4,133,381 to 4,135,624 _18800
Group Condition AO353_18780 AO353_18785 AO353_18790 AO353_18795 AO353_18800
motility outer cut, LB soft agar motility assay -1.5 -2.3 -4.5 -1.0 -2.1
motility outer cut, LB soft agar motility assay -2.3 -3.7 -0.8 -1.6 -2.9
motility outer cut, LB soft agar motility assay -2.5 -2.6 -1.9 -1.2 -2.2
survival 4C survival; 360 hrs -2.6 -2.7 -2.4 +0.3 -1.7
survival 4C survival; 672 hrs -2.3 -2.6 -0.5 +0.5 -1.4
survival 4C survival; 192 hrs -2.1 -0.8 -0.5 +0.1 -0.9
carbon source propionate (C) -3.5 -0.1 -0.3 +0.0 -0.1
stress Cisplatin 0.006 mg/ml -2.7 -0.3 +0.1 +0.1 -0.7
carbon source butyrate (C) -3.0 -0.0 -0.4 +0.0 +0.1
carbon source Cytidine (C) -2.8 +0.0 -0.2 +0.2 -0.2
carbon source propionate (C) -2.9 -0.3 +0.3 +0.0 -0.2
pH Minimal media pH9 -1.5 +0.0 -0.7 -0.3 -0.1
carbon source Uridine (C) -2.7 +0.0 +0.2 +0.0 -0.1
carbon source Ethanol (C) -1.9 -0.1 -0.4 -0.1 -0.1
pH Minimal media pH9 -1.8 +0.3 +0.1 -0.2 -0.6
stress Nickel (II) chloride 0.625 mM -1.3 -0.4 -0.5 -0.1 +0.3
carbon source Ethanol (C) -1.6 +0.2 -0.9 +0.1 +0.2
anaerobic N1B4wt with N2E3m denitrifying replicate C -2.1 +0.1 -0.1 +0.4 -0.2
stress perchlorate 75 mM -1.8 +0.1 -0.2 +0.3 -0.1
stress Cisplatin 0.006 mg/ml -1.7 +0.2 -0.3 -0.2 +0.3
carbon source D-Ribose (C) -1.8 -0.2 +0.4 +0.1 -0.1
stress Benzalkonium Chloride 0.012 mg/ml -2.3 +0.3 -0.3 +0.4 +0.3
carbon source D-Ribose (C) -1.9 -0.1 +0.3 +0.1 +0.2
stress Cholin acetate 38 mM -1.8 +0.1 -0.2 +0.1 +0.3
stress Thallium(I) acetate 0.008 mg/ml -1.4 -0.1 +0.8 -0.1 -0.3
stress Rifampicin 0.005 mg/ml -1.7 +0.4 -0.3 +0.2 +0.4
stress Doxycycline hyclate 0.0006 mg/ml -1.7 +0.5 +0.7 -0.1 +0.4
stress Doxycycline hyclate 0.0002 mg/ml -1.1 +0.2 +0.2 +0.8 +0.2
carbon source L-Citrulline -0.8 +0.7 +0.4 +0.3 +0.4
anaerobic Anaerobic, acetate donor; nitrate and iron +1.1 +0.2 +0.8 +0.1 -0.6
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