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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18780 and AO353_18785 overlap by 4 nucleotides
AO353_18785 and AO353_18790 are separated by 154 nucleotides
AO353_18790 and AO353_18795 are separated by 31 nucleotides
AO353_18795 and AO353_18800 are separated by 38 nucleotides
AO353_18780: AO353_18780 - cobyrinic acid a,c-diamide synthase, at 4,130,417 to 4,131,247
_18780
AO353_18785: AO353_18785 - flagellar biosynthesis sigma factor, at 4,131,244 to 4,131,984
_18785
AO353_18790: AO353_18790 - histidine kinase, at 4,132,139 to 4,132,522
_18790
AO353_18795: AO353_18795 - protein phosphatase, at 4,132,554 to 4,133,342
_18795
AO353_18800: AO353_18800 - chemotaxis protein CheA, at 4,133,381 to 4,135,624
_18800
Group
Condition
AO353
_18780
AO353
_18785
AO353
_18790
AO353
_18795
AO353
_18800
motility
outer cut, LB soft agar motility assay
-1.5
-2.3
-4.5
-1.0
-2.1
motility
outer cut, LB soft agar motility assay
-2.3
-3.7
-0.8
-1.6
-2.9
motility
outer cut, LB soft agar motility assay
-2.5
-2.6
-1.9
-1.2
-2.2
survival
4C survival; 360 hrs
-2.6
-2.7
-2.4
+0.3
-1.7
survival
4C survival; 672 hrs
-2.3
-2.6
-0.5
+0.5
-1.4
survival
4C survival; 192 hrs
-2.1
-0.8
-0.5
+0.1
-0.9
carbon source
propionate (C)
-3.5
-0.1
-0.3
+0.0
-0.1
stress
Cisplatin 0.006 mg/ml
-2.7
-0.3
+0.1
+0.1
-0.7
carbon source
butyrate (C)
-3.0
-0.0
-0.4
+0.0
+0.1
carbon source
Cytidine (C)
-2.8
+0.0
-0.2
+0.2
-0.2
carbon source
propionate (C)
-2.9
-0.3
+0.3
+0.0
-0.2
pH
Minimal media pH9
-1.5
+0.0
-0.7
-0.3
-0.1
carbon source
Uridine (C)
-2.7
+0.0
+0.2
+0.0
-0.1
carbon source
Ethanol (C)
-1.9
-0.1
-0.4
-0.1
-0.1
pH
Minimal media pH9
-1.8
+0.3
+0.1
-0.2
-0.6
stress
Nickel (II) chloride 0.625 mM
-1.3
-0.4
-0.5
-0.1
+0.3
carbon source
Ethanol (C)
-1.6
+0.2
-0.9
+0.1
+0.2
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-2.1
+0.1
-0.1
+0.4
-0.2
stress
perchlorate 75 mM
-1.8
+0.1
-0.2
+0.3
-0.1
stress
Cisplatin 0.006 mg/ml
-1.7
+0.2
-0.3
-0.2
+0.3
carbon source
D-Ribose (C)
-1.8
-0.2
+0.4
+0.1
-0.1
stress
Benzalkonium Chloride 0.012 mg/ml
-2.3
+0.3
-0.3
+0.4
+0.3
carbon source
D-Ribose (C)
-1.9
-0.1
+0.3
+0.1
+0.2
stress
Cholin acetate 38 mM
-1.8
+0.1
-0.2
+0.1
+0.3
stress
Thallium(I) acetate 0.008 mg/ml
-1.4
-0.1
+0.8
-0.1
-0.3
stress
Rifampicin 0.005 mg/ml
-1.7
+0.4
-0.3
+0.2
+0.4
stress
Doxycycline hyclate 0.0006 mg/ml
-1.7
+0.5
+0.7
-0.1
+0.4
stress
Doxycycline hyclate 0.0002 mg/ml
-1.1
+0.2
+0.2
+0.8
+0.2
carbon source
L-Citrulline
-0.8
+0.7
+0.4
+0.3
+0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
+1.1
+0.2
+0.8
+0.1
-0.6
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