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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18775 and AO353_18780 are separated by 114 nucleotides
AO353_18780 and AO353_18785 overlap by 4 nucleotides
AO353_18785 and AO353_18790 are separated by 154 nucleotides
AO353_18790 and AO353_18795 are separated by 31 nucleotides
AO353_18775: AO353_18775 - flagellar biosynthesis protein FlhF, at 4,128,971 to 4,130,302
_18775
AO353_18780: AO353_18780 - cobyrinic acid a,c-diamide synthase, at 4,130,417 to 4,131,247
_18780
AO353_18785: AO353_18785 - flagellar biosynthesis sigma factor, at 4,131,244 to 4,131,984
_18785
AO353_18790: AO353_18790 - histidine kinase, at 4,132,139 to 4,132,522
_18790
AO353_18795: AO353_18795 - protein phosphatase, at 4,132,554 to 4,133,342
_18795
Group
Condition
AO353
_18775
AO353
_18780
AO353
_18785
AO353
_18790
AO353
_18795
motility
outer cut, LB soft agar motility assay
-3.1
-1.5
-2.3
-4.5
-1.0
motility
outer cut, LB soft agar motility assay
-2.9
-2.3
-3.7
-0.8
-1.6
motility
outer cut, LB soft agar motility assay
-1.8
-2.5
-2.6
-1.9
-1.2
survival
4C survival; 360 hrs
-1.3
-2.6
-2.7
-2.4
+0.3
carbon source
propionate (C)
-1.0
-3.5
-0.1
-0.3
+0.0
carbon source
propionate (C)
-1.4
-2.9
-0.3
+0.3
+0.0
survival
4C survival; 672 hrs
+1.0
-2.3
-2.6
-0.5
+0.5
carbon source
butyrate (C)
-0.6
-3.0
-0.0
-0.4
+0.0
survival
4C survival; 192 hrs
-0.7
-2.1
-0.8
-0.5
+0.1
carbon source
Cytidine (C)
-1.1
-2.8
+0.0
-0.2
+0.2
stress
Cisplatin 0.006 mg/ml
-1.0
-2.7
-0.3
+0.1
+0.1
stress
Cisplatin 0.006 mg/ml
-1.5
-1.7
+0.2
-0.3
-0.2
stress
perchlorate 75 mM
-1.8
-1.8
+0.1
-0.2
+0.3
carbon source
Uridine (C)
-0.6
-2.7
+0.0
+0.2
+0.0
carbon source
L-Leucine (C)
-1.0
-1.9
+0.0
-0.0
+0.2
pH
Minimal media pH9
-0.9
-1.8
+0.3
+0.1
-0.2
stress
nitrate 200 mM
-1.6
-1.2
+0.2
+0.1
+0.2
stress
Thallium(I) acetate 0.016 mg/ml
-0.9
-1.9
+0.2
+0.2
-0.0
stress
Benzalkonium Chloride 0.012 mg/ml
-0.3
-2.3
+0.3
-0.3
+0.4
carbon source
D-Glucosamine Hydrochloride (C)
-1.1
-1.3
+0.7
+0.0
-0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.7
-1.4
-0.1
+0.8
-0.1
carbon source
L-Ornithine
-0.8
-1.2
+0.3
+0.2
+0.6
stress
Doxycycline hyclate 0.0006 mg/ml
+0.1
-1.7
+0.5
+0.7
-0.1
stress
Cholin acetate 38 mM
+1.9
-1.8
+0.1
-0.2
+0.1
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+1.6
-1.1
+0.3
-0.4
-0.2
stress
Cholin acetate 10 mM
+2.3
-1.3
+0.1
-0.0
+0.3
stress
Cholin acetate 19 mM
+3.0
-1.1
+0.1
-0.4
+0.2
stress
1-ethyl-3-methylimidazolium acetate 15 mM
+3.0
-1.4
+0.3
-0.1
+0.1
stress
acetate 34 mM
+3.1
-1.1
+0.0
-0.2
+0.3
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+2.9
-0.9
+0.2
-0.0
+0.3
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