Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18750 and AO353_18755 are separated by 3 nucleotidesAO353_18755 and AO353_18760 are separated by 3 nucleotidesAO353_18760 and AO353_18765 are separated by 554 nucleotidesAO353_18765 and AO353_18770 are separated by 420 nucleotides AO353_18750: AO353_18750 - flagellar biosynthetic protein FliQ, at 4,123,417 to 4,123,686 _18750 AO353_18755: AO353_18755 - flagellar biosynthetic protein FliR, at 4,123,690 to 4,124,472 _18755 AO353_18760: AO353_18760 - flagellar biosynthetic protein FlhB, at 4,124,476 to 4,125,615 _18760 AO353_18765: AO353_18765 - hypothetical protein, at 4,126,170 to 4,126,409 _18765 AO353_18770: AO353_18770 - flagellar biosynthesis protein FlhA, at 4,126,830 to 4,128,959 _18770
Group Condition AO353_18750 AO353_18755 AO353_18760 AO353_18765 AO353_18770
motility outer cut, LB soft agar motility assay -2.7 -3.2 -2.8 +0.6 -2.5
motility outer cut, LB soft agar motility assay -2.1 -3.0 -3.0 +1.3 -2.3
motility outer cut, LB soft agar motility assay -2.6 -2.0 -1.7 -0.5 -1.2
survival 4C survival; 360 hrs -1.6 -2.0 -1.6 -0.3 -1.8
survival 4C survival; 672 hrs -1.7 -1.6 -1.6 -0.3 -1.1
survival 4C survival; 192 hrs -1.1 -0.9 -1.2 -0.4 -1.4
stress Nickel (II) chloride 0.625 mM +0.1 +0.2 -0.4 -1.5 -0.2
lb LB -0.3 +0.4 -0.0 -0.8 -0.0
stress Doxycycline hyclate 0.0006 mg/ml -0.0 +0.1 +0.1 -1.3 +0.6
stress Cisplatin 0.006 mg/ml -0.1 +0.3 +0.3 -0.8 +0.2
stress Sisomicin 0.008 mg/ml +0.1 -0.3 -0.5 +0.8 -0.1
carbon source L-Malic (C) +0.1 -0.5 -0.3 +0.6 +0.1
denitrifying Nitrate reduction, replicate B -0.2 +0.4 +0.4 -0.6 +0.0
carbon source L-Citrulline +0.5 +0.1 +0.2 -0.8 +0.2
stress Thallium(I) acetate 0.016 mg/ml +0.3 +0.4 +0.2 -0.8 +0.2
carbon source L-Citrulline +0.9 +0.4 +0.4 -1.3 +0.1
stress Cisplatin 0.006 mg/ml +0.7 +0.7 +0.1 -0.9 +0.2
anaerobic Anaerobic, acetate donor; nitrate and iron +0.9 -0.6 -0.1 +0.7 +0.3
stress Lomefloxacin 0.001 mM +0.6 +0.1 +0.1 +0.6 +0.3
stress acetate 68 mM +0.4 +0.4 -0.0 +0.4 +0.5
nitrogen source Urea (N) +0.4 +0.5 +0.4 -0.0 +0.5
stress 1-ethyl-3-methylimidazolium acetate 20 mM +0.2 +0.5 +0.5 -0.4 +1.1
stress acetate 17 mM +0.3 +0.2 +0.0 +0.5 +0.8
carbon source a-Ketoglutaric (C) +0.3 +0.5 +0.5 +0.0 +0.5
stress acetate 34 mM +0.4 +0.3 +0.4 -0.0 +0.9
stress Cholin acetate 19 mM +0.7 +0.6 +0.5 -0.3 +1.1
carbon source D-Glucosamine Hydrochloride (C) +0.8 +0.8 +0.8 -0.3 +0.6
stress 1-ethyl-3-methylimidazolium acetate 30 mM +0.3 +0.5 +0.3 +1.0 +0.7
stress 1-ethyl-3-methylimidazolium acetate 15 mM +0.7 +0.4 +0.2 +0.4 +1.1
stress Rifampicin 0.005 mg/ml +0.5 +0.7 +0.8 +0.5 +0.7
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