Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18715 and AO353_18720 are separated by 105 nucleotidesAO353_18720 and AO353_18725 are separated by 200 nucleotidesAO353_18725 and AO353_18730 are separated by 9 nucleotidesAO353_18730 and AO353_18735 are separated by 69 nucleotides AO353_18715: AO353_18715 - histidine kinase, at 4,118,142 to 4,118,486 _18715 AO353_18720: AO353_18720 - flagellar hook-length control protein, at 4,118,592 to 4,120,001 _18720 AO353_18725: AO353_18725 - flagellar basal body protein FliL, at 4,120,202 to 4,120,702 _18725 AO353_18730: AO353_18730 - flagellar motor switch protein FliM, at 4,120,712 to 4,121,680 _18730 AO353_18735: AO353_18735 - flagellar motor switch protein, at 4,121,750 to 4,122,211 _18735
Group Condition AO353_18715 AO353_18720 AO353_18725 AO353_18730 AO353_18735
motility outer cut, LB soft agar motility assay -4.3 -2.7 +0.5 -3.4 N.D.
survival 4C survival; 672 hrs -2.7 -1.5 -1.3 -2.3 N.D.
motility outer cut, LB soft agar motility assay -1.9 -2.4 +0.0 -2.5 N.D.
survival 4C survival; 360 hrs -1.3 -1.5 -1.5 -1.9 N.D.
motility outer cut, LB soft agar motility assay -1.1 -2.8 +0.2 -2.3 N.D.
pH Minimal media pH9 -4.0 +0.0 -0.2 -0.5 N.D.
nitrogen source Putrescine (N) -3.5 -0.1 +0.2 -0.1 N.D.
carbon source L-Ornithine -3.7 +0.3 +0.4 -0.4 N.D.
stress Doxycycline hyclate 0.0006 mg/ml -2.8 -0.1 -0.7 +0.2 N.D.
survival 4C survival; 192 hrs +0.1 -1.3 -1.0 -0.9 N.D.
carbon source L-Asparagine (C) -3.0 +0.0 +0.4 +0.0 N.D.
carbon source L-Citrulline -3.3 +0.1 +0.4 +0.2 N.D.
stress perchlorate 75 mM -2.2 -0.1 +0.5 -0.3 N.D.
carbon source L-Valine (C) -1.1 -0.3 -0.7 +0.2 N.D.
carbon source D-Lactate (C) -1.9 +0.1 +0.2 -0.2 N.D.
carbon source D-Glucose (C) -2.1 +0.0 +0.2 +0.1 N.D.
anaerobic Anaerobic, acetate donor; nitrate and iron -2.8 +0.1 +0.7 +0.4 N.D.
stress methylglyoxal 0.009 vol% -1.4 -0.1 +0.4 -0.5 N.D.
carbon source acetate (C) -1.7 -0.0 +0.5 -0.3 N.D.
carbon source D-Trehalose (C) -1.9 -0.1 +0.3 +0.1 N.D.
carbon source m-Inositol (C) -1.9 +0.2 +0.4 +0.2 N.D.
carbon source L-Ornithine -1.9 +0.4 +0.3 -0.0 N.D.
carbon source L-Ornithine -1.5 +0.3 +0.5 -0.3 N.D.
stress Spectinomycin 0.2 mg/ml -1.8 +0.2 +0.4 +0.4 N.D.
carbon source m-Inositol (C) -1.6 +0.3 +0.5 -0.0 N.D.
stress Cisplatin 0.006 mg/ml +1.7 +0.1 +0.2 -0.4 N.D.
stress acetate 34 mM +0.5 -0.3 +0.5 +1.2 N.D.
carbon source D-Glucosamine Hydrochloride (C) +0.5 +0.7 +0.6 +0.6 N.D.
stress Cisplatin 0.006 mg/ml +1.0 +0.3 +0.3 +0.9 N.D.
stress Cholin acetate 19 mM +0.3 +0.2 +0.6 +1.4 N.D.
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