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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18685 and AO353_18690 are separated by 4 nucleotides
AO353_18690 and AO353_18695 overlap by 11 nucleotides
AO353_18695 and AO353_18700 are separated by 6 nucleotides
AO353_18700 and AO353_18705 are separated by 77 nucleotides
AO353_18685: AO353_18685 - flagellar motor switch protein FliG, at 4,112,376 to 4,113,395
_18685
AO353_18690: AO353_18690 - flagellar assembly protein FliH, at 4,113,400 to 4,114,209
_18690
AO353_18695: AO353_18695 - flagellar protein export ATPase FliI, at 4,114,199 to 4,115,557
_18695
AO353_18700: AO353_18700 - flagellar biogenesis protein, at 4,115,564 to 4,116,013
_18700
AO353_18705: AO353_18705 - anti-anti-sigma factor, at 4,116,091 to 4,116,399
_18705
Group
Condition
AO353
_18685
AO353
_18690
AO353
_18695
AO353
_18700
AO353
_18705
motility
outer cut, LB soft agar motility assay
-2.7
-1.5
-2.1
-2.6
-0.9
motility
outer cut, LB soft agar motility assay
-2.5
-2.0
-2.6
-2.0
+0.3
motility
outer cut, LB soft agar motility assay
-2.3
-2.5
-1.5
-0.3
+0.0
survival
4C survival; 360 hrs
-2.5
-1.5
-1.5
-1.1
+1.2
survival
4C survival; 192 hrs
-1.3
-0.8
-1.0
-0.7
-0.2
survival
4C survival; 672 hrs
-1.7
-1.5
-1.5
-2.3
+4.2
carbon source
D-Glucose
-0.1
+0.4
-0.0
-0.5
-1.3
carbon source
Uridine (C)
+0.1
-0.1
+0.2
-0.2
-1.6
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.1
+0.4
-0.1
-0.7
-0.8
carbon source
L-Isoleucine (C)
+0.1
+0.1
+0.2
-0.1
-1.5
carbon source
L-Citrulline
+0.2
+0.2
+0.3
-1.6
-0.2
denitrifying
Nitrate reduction, replicate C
+0.6
-0.3
-0.1
-1.3
+0.3
carbon source
m-Inositol (C)
+0.3
+0.1
+0.3
+0.2
-1.4
stress
Cholin acetate 38 mM
+0.6
-0.1
+0.2
-1.1
+0.1
nitrogen source
Inosine (N)
+0.3
+0.5
+0.4
-0.7
-0.8
pH
Minimal media pH6
+0.1
+0.5
+0.1
+0.4
-1.1
stress
acetate 34 mM
+0.9
-0.1
+0.2
-0.7
-0.3
carbon source
L-Proline (C)
+0.2
-0.2
+0.0
+0.9
-0.8
carbon source
m-Inositol (C)
+0.1
+0.4
+0.1
+0.7
-1.1
carbon source
L-Leucine (C)
+0.4
+0.2
-0.1
+0.9
-1.0
denitrifying
Nitrate reduction, replicate B
+0.7
-0.4
+0.3
-0.5
+0.3
nitrogen source
Urea (N)
+0.2
+0.3
+0.4
+0.9
-1.2
stress
1-ethyl-3-methylimidazolium acetate 15 mM
+1.1
-0.1
+0.4
-0.8
+0.2
stress
Tetracycline 0.0016 mg/ml
+0.3
+0.8
+0.4
-0.5
+0.3
carbon source
acetate (C)
+0.4
-0.4
+0.3
+0.4
+0.5
stress
Cholin acetate 19 mM
+0.9
+0.4
+0.4
-0.4
-0.1
stress
Spectinomycin 0.2 mg/ml
+0.5
+0.5
+0.2
+0.6
-0.4
carbon source
D-Glucosamine Hydrochloride (C)
+0.8
+0.8
+0.7
+1.0
-1.6
stress
Lomefloxacin 0.001 mM
+0.2
+0.7
+0.6
-0.4
+0.7
stress
Rifampicin 0.005 mg/ml
+1.0
+1.1
+0.8
-0.4
+0.4
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