Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18635 and AO353_18640 are separated by 72 nucleotidesAO353_18640 and AO353_18645 are separated by 81 nucleotidesAO353_18645 and AO353_18650 are separated by 153 nucleotidesAO353_18650 and AO353_18655 are separated by 24 nucleotides AO353_18635: AO353_18635 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 4,101,371 to 4,102,816 _18635 AO353_18640: AO353_18640 - flagellar biosynthesis protein FlaG, at 4,102,889 to 4,103,239 _18640 AO353_18645: AO353_18645 - flagellar cap protein FliD, at 4,103,321 to 4,104,721 _18645 AO353_18650: AO353_18650 - flagellar export chaperone FliS, at 4,104,875 to 4,105,264 _18650 AO353_18655: AO353_18655 - flagellar assembly protein FliT, at 4,105,289 to 4,105,585 _18655
Group Condition AO353_18635 AO353_18640 AO353_18645 AO353_18650 AO353_18655
motility outer cut, LB soft agar motility assay -2.9 -0.5 -2.3 -3.4 +0.1
motility outer cut, LB soft agar motility assay -2.5 -0.0 -3.2 -2.2 +0.2
survival 4C survival; 360 hrs -2.0 +0.1 -2.0 -2.2 -1.2
survival 4C survival; 672 hrs -1.6 +0.3 -1.4 -2.0 -1.4
survival 4C survival; 192 hrs -1.1 -0.3 -1.4 -1.2 -0.6
motility outer cut, LB soft agar motility assay -1.3 -0.3 -2.2 -2.0 +1.6
stress Cholin acetate 19 mM -0.1 +0.0 -0.1 -0.2 -1.3
anaerobic Anaerobic, acetate donor; nitrate and iron -0.5 -0.9 +0.1 -0.0 -0.2
carbon source D-Trehalose (C) +0.0 -0.4 +0.0 +0.0 -0.9
stress 1-ethyl-3-methylimidazolium acetate 30 mM -0.1 -0.1 +0.4 -0.2 -1.0
stress Rifampicin 0.005 mg/ml -0.5 -0.3 -0.1 +0.3 -0.4
temperature LB 10C +0.2 -0.4 +0.1 -0.0 -0.7
nitrogen source Parabanic Acid (N) +0.2 +0.1 +0.4 -0.1 -1.4
stress perchlorate 75 mM +0.2 -0.1 +0.2 +0.3 -0.8
stress Cisplatin 0.006 mg/ml +0.4 -0.0 +0.3 -0.1 -0.7
carbon source L-Ornithine +0.5 -0.6 +0.5 -0.1 -0.1
carbon source D-Glucose +0.4 -0.3 +0.1 +0.4 -0.4
carbon source D-Mannose (C) -0.2 -0.2 -0.1 +0.0 +0.8
carbon source L-Phenylalanine (C) +0.1 -0.3 +0.3 -0.3 +0.5
carbon source L-Ornithine +0.6 -0.1 +0.3 -0.2 -0.2
nitrogen source Thymine (N) -0.1 -0.3 -0.0 +0.2 +0.7
carbon source L-Ornithine +0.6 -0.2 +0.4 -0.1 +0.2
nitrogen source Inosine (N) +0.3 -0.3 +0.2 +0.1 +0.9
carbon source m-Inositol (C) +0.2 -0.2 +0.4 +0.3 +0.5
carbon source L-Citrulline +0.6 -0.1 +0.0 +0.1 +0.7
carbon source L-Isoleucine (C) +0.1 +0.3 -0.1 +0.2 +0.9
carbon source D-Glucosamine Hydrochloride (C) +0.2 +0.1 +0.2 +0.5 +0.5
carbon source acetate (C) +0.0 +0.5 +0.1 +0.2 +0.7
carbon source pyruvate (C) +0.1 +0.3 +0.1 +0.2 +1.0
carbon source a-Ketoglutaric (C) +0.3 -0.1 +0.4 +0.8 +1.4
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