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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18635 and AO353_18640 are separated by 72 nucleotides
AO353_18640 and AO353_18645 are separated by 81 nucleotides
AO353_18645 and AO353_18650 are separated by 153 nucleotides
AO353_18650 and AO353_18655 are separated by 24 nucleotides
AO353_18635: AO353_18635 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 4,101,371 to 4,102,816
_18635
AO353_18640: AO353_18640 - flagellar biosynthesis protein FlaG, at 4,102,889 to 4,103,239
_18640
AO353_18645: AO353_18645 - flagellar cap protein FliD, at 4,103,321 to 4,104,721
_18645
AO353_18650: AO353_18650 - flagellar export chaperone FliS, at 4,104,875 to 4,105,264
_18650
AO353_18655: AO353_18655 - flagellar assembly protein FliT, at 4,105,289 to 4,105,585
_18655
Group
Condition
AO353
_18635
AO353
_18640
AO353
_18645
AO353
_18650
AO353
_18655
motility
outer cut, LB soft agar motility assay
-2.9
-0.5
-2.3
-3.4
+0.1
motility
outer cut, LB soft agar motility assay
-2.5
-0.0
-3.2
-2.2
+0.2
survival
4C survival; 360 hrs
-2.0
+0.1
-2.0
-2.2
-1.2
survival
4C survival; 672 hrs
-1.6
+0.3
-1.4
-2.0
-1.4
survival
4C survival; 192 hrs
-1.1
-0.3
-1.4
-1.2
-0.6
motility
outer cut, LB soft agar motility assay
-1.3
-0.3
-2.2
-2.0
+1.6
stress
Cholin acetate 19 mM
-0.1
+0.0
-0.1
-0.2
-1.3
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.5
-0.9
+0.1
-0.0
-0.2
carbon source
D-Trehalose (C)
+0.0
-0.4
+0.0
+0.0
-0.9
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.1
-0.1
+0.4
-0.2
-1.0
stress
Rifampicin 0.005 mg/ml
-0.5
-0.3
-0.1
+0.3
-0.4
temperature
LB 10C
+0.2
-0.4
+0.1
-0.0
-0.7
nitrogen source
Parabanic Acid (N)
+0.2
+0.1
+0.4
-0.1
-1.4
stress
perchlorate 75 mM
+0.2
-0.1
+0.2
+0.3
-0.8
stress
Cisplatin 0.006 mg/ml
+0.4
-0.0
+0.3
-0.1
-0.7
carbon source
L-Ornithine
+0.5
-0.6
+0.5
-0.1
-0.1
carbon source
D-Glucose
+0.4
-0.3
+0.1
+0.4
-0.4
carbon source
D-Mannose (C)
-0.2
-0.2
-0.1
+0.0
+0.8
carbon source
L-Phenylalanine (C)
+0.1
-0.3
+0.3
-0.3
+0.5
carbon source
L-Ornithine
+0.6
-0.1
+0.3
-0.2
-0.2
nitrogen source
Thymine (N)
-0.1
-0.3
-0.0
+0.2
+0.7
carbon source
L-Ornithine
+0.6
-0.2
+0.4
-0.1
+0.2
nitrogen source
Inosine (N)
+0.3
-0.3
+0.2
+0.1
+0.9
carbon source
m-Inositol (C)
+0.2
-0.2
+0.4
+0.3
+0.5
carbon source
L-Citrulline
+0.6
-0.1
+0.0
+0.1
+0.7
carbon source
L-Isoleucine (C)
+0.1
+0.3
-0.1
+0.2
+0.9
carbon source
D-Glucosamine Hydrochloride (C)
+0.2
+0.1
+0.2
+0.5
+0.5
carbon source
acetate (C)
+0.0
+0.5
+0.1
+0.2
+0.7
carbon source
pyruvate (C)
+0.1
+0.3
+0.1
+0.2
+1.0
carbon source
a-Ketoglutaric (C)
+0.3
-0.1
+0.4
+0.8
+1.4
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