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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18625 and AO353_18630 are separated by 317 nucleotides
AO353_18630 and AO353_18635 are separated by 251 nucleotides
AO353_18635 and AO353_18640 are separated by 72 nucleotides
AO353_18640 and AO353_18645 are separated by 81 nucleotides
AO353_18625: AO353_18625 - flagellar biosynthesis protein FlgL, at 4,098,298 to 4,099,872
_18625
AO353_18630: AO353_18630 - beta-ketoacyl-ACP synthase, at 4,100,190 to 4,101,119
_18630
AO353_18635: AO353_18635 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 4,101,371 to 4,102,816
_18635
AO353_18640: AO353_18640 - flagellar biosynthesis protein FlaG, at 4,102,889 to 4,103,239
_18640
AO353_18645: AO353_18645 - flagellar cap protein FliD, at 4,103,321 to 4,104,721
_18645
Group
Condition
AO353
_18625
AO353
_18630
AO353
_18635
AO353
_18640
AO353
_18645
motility
outer cut, LB soft agar motility assay
-2.9
-1.9
-2.9
-0.5
-2.3
motility
outer cut, LB soft agar motility assay
-2.7
-1.7
-2.5
-0.0
-3.2
motility
outer cut, LB soft agar motility assay
-2.3
-1.3
-1.3
-0.3
-2.2
survival
4C survival; 360 hrs
-1.6
-0.8
-2.0
+0.1
-2.0
survival
4C survival; 672 hrs
-1.8
-1.0
-1.6
+0.3
-1.4
stress
Rifampicin 0.005 mg/ml
-0.4
-3.5
-0.5
-0.3
-0.1
survival
4C survival; 192 hrs
-1.5
-0.5
-1.1
-0.3
-1.4
stress
Doxycycline hyclate 0.0002 mg/ml
-0.0
-2.8
-0.4
+0.2
-0.1
stress
Ceftazidime 0.12 mg/ml
-0.1
-2.2
-0.1
-0.2
+0.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.0
-2.1
-0.3
-0.0
+0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.1
-0.6
-0.5
-0.9
+0.1
stress
Vancomycin 1 mg/ml
+0.1
-1.8
+0.0
-0.3
+0.2
stress
Sisomicin 0.008 mg/ml
+0.2
-1.1
-0.2
-0.2
-0.1
stress
Lomefloxacin 0.001 mM
+0.2
-1.6
+0.2
-0.2
+0.2
stress
Tetracycline 0.0016 mg/ml
+0.2
-1.2
-0.2
+0.3
+0.2
stress
Nickel (II) chloride 0.625 mM
-0.2
-1.0
+0.2
+0.1
+0.2
stress
methylglyoxal 0.009 vol%
+0.1
+1.3
+0.0
+0.2
+0.1
stress
Cisplatin 0.006 mg/ml
+0.3
+0.9
+0.4
-0.0
+0.3
carbon source
D-Glucose
+0.2
+1.5
+0.4
-0.3
+0.1
carbon source
acetate (C)
+0.0
+1.3
+0.2
+0.2
+0.2
carbon source
acetate (C)
-0.1
+1.5
+0.0
+0.5
+0.1
carbon source
L-Citrulline
+0.1
+1.7
+0.4
-0.0
-0.2
carbon source
L-Ornithine
+0.0
+1.3
+0.6
-0.2
+0.4
carbon source
D-Glucose
+0.3
+1.4
+0.2
+0.2
+0.2
carbon source
L-Ornithine
+0.2
+1.7
+0.5
-0.6
+0.5
carbon source
L-Ornithine
+0.0
+1.6
+0.5
+0.1
+0.0
carbon source
L-Citrulline
-0.1
+1.7
+0.2
+0.3
+0.2
carbon source
L-Citrulline
+0.2
+1.7
+0.6
-0.1
+0.0
carbon source
L-Ornithine
+0.5
+1.6
+0.6
-0.1
+0.3
carbon source
L-Citrulline
+0.2
+1.9
+0.4
+0.1
+0.4
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