Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_18450 and AO353_18455 are separated by 91 nucleotidesAO353_18455 and AO353_18460 are separated by 134 nucleotidesAO353_18460 and AO353_18465 are separated by 176 nucleotidesAO353_18465 and AO353_18470 are separated by 99 nucleotides AO353_18450: AO353_18450 - hypothetical protein, at 4,064,379 to 4,065,272 _18450 AO353_18455: AO353_18455 - AraC family transcriptional regulator, at 4,065,364 to 4,066,122 _18455 AO353_18460: AO353_18460 - serine/threonine protein phosphatase, at 4,066,257 to 4,067,072 _18460 AO353_18465: AO353_18465 - phosphohydrolase, at 4,067,249 to 4,067,839 _18465 AO353_18470: AO353_18470 - hypothetical protein, at 4,067,939 to 4,068,250 _18470
Group Condition AO353_18450 AO353_18455 AO353_18460 AO353_18465 AO353_18470
pH Minimal media pH9 -1.7 -0.1 -0.2 -0.1 -0.4
carbon source acetate (C) -0.9 -0.1 -0.4 -0.4 +0.1
motility outer cut, LB soft agar motility assay +0.5 -1.1 -0.5 +0.3 -0.6
nitrogen source L-Glutamine (N) -1.0 -0.3 -0.2 +0.3 -0.1
carbon source L-Citrulline -0.6 -1.0 +0.4 +0.5 -0.3
carbon source L-Ornithine -0.4 -0.1 -0.2 -0.6 +0.3
carbon source D-Trehalose (C) -1.6 -0.1 +0.0 +0.6 +0.2
carbon source L-Citrulline -0.4 +0.2 +0.4 -0.6 -0.3
carbon source L-Alanine (C) -0.3 +0.3 -0.3 -0.3 -0.2
stress 1-ethyl-3-methylimidazolium chloride 170 mM -0.7 -0.1 -0.3 -0.1 +0.4
stress methylglyoxal 0.009 vol% +0.8 -0.3 -0.2 -0.5 -0.3
stress Lomefloxacin 0.001 mM -0.5 +0.3 -0.3 +0.1 -0.2
stress Cisplatin 0.006 mg/ml -1.1 +0.6 +0.6 +0.3 -0.7
carbon source D-Trehalose (C) -0.8 +0.0 +0.1 +0.6 -0.1
radiation Ionizing radiation, 126.196 Gy, -0.5 +0.6 +0.1 -0.1 -0.3
survival 4C survival; 672 hrs -0.3 -0.1 -0.1 +0.8 -0.3
anaerobic N1B4wt with N2E3m denitrifying replicate C +0.2 +0.3 +0.2 +0.0 -0.7
denitrifying Nitrate reduction, replicate B -0.8 +0.2 +0.1 +0.5 +0.0
carbon source L-Citrulline -0.2 -0.5 +0.2 +0.3 +0.2
survival 4C survival; 360 hrs -0.8 +0.0 +0.2 +0.5 +0.2
stress Cisplatin 0.006 mg/ml -0.7 +0.1 +0.4 +0.3 +0.0
stress Sisomicin 0.008 mg/ml -0.6 -0.0 +0.1 +1.2 -0.5
denitrifying Nitrate reduction, replicate C -0.4 +0.2 +0.1 +0.6 -0.3
carbon source L-Citrulline -0.3 -0.1 +0.3 -0.2 +0.6
carbon source propionate (C) -0.4 +0.1 +0.1 +0.7 +0.1
carbon source L-Serine (C) -0.6 +0.2 -0.0 +1.5 +0.3
motility outer cut, LB soft agar motility assay +0.1 +0.2 +0.3 +0.7 +0.2
stress Doxycycline hyclate 0.0006 mg/ml +0.8 +0.3 -0.4 +0.4 +0.4
carbon source L-Isoleucine (C) +1.0 +0.1 -0.0 +0.4 +0.1
anaerobic Anaerobic, acetate donor; nitrate and iron +0.1 +0.4 +0.3 +1.0 +0.3
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