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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_18215 and AO353_18220 are separated by 129 nucleotides
AO353_18220 and AO353_18225 are separated by 190 nucleotides
AO353_18225 and AO353_18230 are separated by 395 nucleotides
AO353_18230 and AO353_18235 are separated by 54 nucleotides
AO353_18215: AO353_18215 - multidrug DMT transporter permease, at 4,013,993 to 4,014,901
_18215
AO353_18220: AO353_18220 - isocitrate dehydrogenase, at 4,015,031 to 4,016,752
_18220
AO353_18225: AO353_18225 - ATP-dependent RNA helicase HrpA, at 4,016,943 to 4,020,854
_18225
AO353_18230: AO353_18230 - 3-oxoacyl-ACP synthase, at 4,021,250 to 4,022,371
_18230
AO353_18235: AO353_18235 - RNA polymerase subunit sigma-24, at 4,022,426 to 4,023,034
_18235
Group
Condition
AO353
_18215
AO353
_18220
AO353
_18225
AO353
_18230
AO353
_18235
carbon source
Tween 20 (C)
-0.2
-3.0
-0.4
-0.5
-0.2
carbon source
L-Leucine (C)
-0.3
-3.7
+0.1
-0.0
-0.1
carbon source
Tween 20 (C)
-0.3
-3.0
-0.2
-0.3
+0.0
carbon source
Ethanol (C)
-0.3
-1.5
+0.1
-0.1
-0.1
carbon source
Ethanol (C)
-0.3
-1.3
-0.0
+0.0
-0.1
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.0
+0.0
-1.4
-0.4
+0.1
stress
Tetracycline 0.0016 mg/ml
-0.4
-0.1
-0.4
-0.7
-0.1
carbon source
L-Isoleucine (C)
-0.6
-0.3
-0.4
-0.0
-0.1
stress
methylglyoxal 0.009 vol%
-0.5
+0.0
-0.3
-0.6
-0.1
stress
Rifampicin 0.005 mg/ml
+0.2
+0.0
-0.5
-0.4
-0.7
stress
Cobalt chloride 0.16 mM
-0.1
+0.1
-1.0
-0.0
-0.2
carbon source
acetate (C)
-0.4
-0.8
+0.1
-0.1
+0.0
stress
Doxycycline hyclate 0.0002 mg/ml
+0.1
+0.0
-0.4
-0.7
-0.1
stress
Spectinomycin 0.2 mg/ml
-0.1
+0.1
-0.8
-0.2
+0.0
stress
Fusidic 0.125 mg/ml
+0.9
+0.8
-2.4
-0.2
+0.1
stress
Spectinomycin 0.15 mg/ml
+0.2
+0.2
-0.6
-0.3
-0.2
pH
Minimal media pH9
-0.8
-0.3
+0.2
-0.1
+0.3
stress
Lomefloxacin 0.001 mM
+0.7
-0.2
-0.2
-0.4
-0.3
stress
Benzalkonium Chloride 0.01 mg/ml
+0.2
-0.1
-0.4
+0.6
-0.2
carbon source
acetate (C)
+0.3
-0.5
-0.0
+0.1
+0.3
stress
Doxycycline hyclate 0.0006 mg/ml
-0.0
+0.1
+0.7
-0.8
+0.5
carbon source
L-Ornithine
-0.2
-0.1
+0.5
-0.1
+0.5
stress
Cisplatin 0.006 mg/ml
+0.3
-0.1
+0.6
-0.3
+0.2
stress
nitrate 200 mM
-0.5
-0.0
+0.8
+0.4
+0.1
stress
Cisplatin 0.006 mg/ml
+0.2
+0.2
+0.6
-0.2
+0.1
motility
outer cut, LB soft agar motility assay
+0.4
+0.2
+0.6
-0.1
-0.1
carbon source
L-Ornithine
+0.0
-0.2
+0.6
+0.2
+0.3
stress
Benzalkonium Chloride 0.012 mg/ml
+0.5
+0.1
-0.1
+0.7
-0.0
survival
4C survival; 672 hrs
+0.3
+0.1
-0.1
+0.4
+0.5
stress
perchlorate 75 mM
+0.1
+0.2
+1.0
+0.1
-0.1
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