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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_17210 and AO353_17215 are separated by 1 nucleotides
AO353_17215 and AO353_17220 are separated by 254 nucleotides
AO353_17220 and AO353_17225 are separated by 49 nucleotides
AO353_17225 and AO353_17230 are separated by 12 nucleotides
AO353_17210: AO353_17210 - protocatechuate 3,4-dioxygenase, at 3,805,592 to 3,806,296
_17210
AO353_17215: AO353_17215 - protocatechuate 3,4-dioxygenase, at 3,806,298 to 3,806,864
_17215
AO353_17220: AO353_17220 - alpha-ketoglutarate permease, at 3,807,119 to 3,808,414
_17220
AO353_17225: AO353_17225 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,808,464 to 3,809,825
_17225
AO353_17230: AO353_17230 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24)
(from data)
, at 3,809,838 to 3,810,638
_17230
Group
Condition
AO353
_17210
AO353
_17215
AO353
_17220
AO353
_17225
AO353
_17230
carbon source
L-Tryptophan (C)
+0.3
-0.0
-0.8
-0.2
-3.0
nitrogen source
L-Tryptophan (N)
-0.0
-0.0
-0.3
-0.4
-1.0
carbon source
L-Valine (C)
-0.4
-0.0
-0.1
-0.2
-0.3
stress
Sisomicin 0.008 mg/ml
-0.6
-0.3
-0.1
-0.1
+0.2
stress
Cisplatin 0.006 mg/ml
-0.3
-0.7
-0.0
-0.3
+0.5
stress
Lomefloxacin 0.001 mM
+0.0
-0.1
-0.3
-0.1
-0.4
denitrifying
Nitrate reduction, replicate B
-0.2
-0.2
+0.1
-0.4
+0.0
carbon source
L-Phenylalanine (C)
-0.2
+0.4
+0.0
-0.3
-0.5
carbon source
L-Ornithine
+0.0
-0.8
+0.0
+0.2
-0.0
stress
Doxycycline hyclate 0.0006 mg/ml
+0.2
-1.2
+0.1
+0.2
+0.2
carbon source
Ethanol (C)
-0.3
+0.1
+0.1
-0.3
-0.1
survival
4C survival; 360 hrs
+0.3
-0.4
-0.2
+0.1
-0.1
motility
outer cut, LB soft agar motility assay
-0.0
+0.3
+0.1
-0.7
-0.0
stress
acetate 34 mM
-0.2
+0.1
-0.0
-0.3
+0.3
stress
Cisplatin 0.006 mg/ml
+0.4
-0.2
-0.0
-0.5
+0.1
pH
Minimal media pH9
-0.0
-0.5
-0.1
+0.1
+0.4
carbon source
L-Citrulline
-0.3
+0.6
-0.1
-0.2
-0.1
nitrogen source
L-Lysine (N)
-0.1
+0.3
-0.1
-0.3
+0.1
carbon source
L-Ornithine
-0.2
-0.3
+0.0
+0.6
-0.1
carbon source
L-Aspartic Acid (C)
+0.0
+0.3
+0.2
-0.1
-0.3
survival
4C survival; 672 hrs
+0.5
-0.2
-0.1
+0.2
-0.0
carbon source
L-Citrulline
+0.1
+0.4
-0.0
+0.5
-0.6
carbon source
L-Citrulline
+0.6
-0.3
+0.1
+0.0
+0.1
motility
outer cut, LB soft agar motility assay
+0.3
+0.2
-0.2
+0.1
+0.4
carbon source
D-Glucose
-0.1
+0.4
+0.2
+0.2
+0.1
carbon source
D-Glucose
+0.4
-0.2
+0.2
+0.5
+0.0
nitrogen source
L-Arginine (N)
+0.3
+0.5
+0.0
+0.1
+0.1
carbon source
acetate (C)
+0.2
+0.3
+0.1
+0.3
+0.3
stress
Benzalkonium Chloride 0.012 mg/ml
+0.5
+0.1
+0.1
+0.2
+0.3
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.5
+0.6
+0.3
+0.2
+0.0
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