Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_17210 and AO353_17215 are separated by 1 nucleotidesAO353_17215 and AO353_17220 are separated by 254 nucleotidesAO353_17220 and AO353_17225 are separated by 49 nucleotidesAO353_17225 and AO353_17230 are separated by 12 nucleotides AO353_17210: AO353_17210 - protocatechuate 3,4-dioxygenase, at 3,805,592 to 3,806,296 _17210 AO353_17215: AO353_17215 - protocatechuate 3,4-dioxygenase, at 3,806,298 to 3,806,864 _17215 AO353_17220: AO353_17220 - alpha-ketoglutarate permease, at 3,807,119 to 3,808,414 _17220 AO353_17225: AO353_17225 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,808,464 to 3,809,825 _17225 AO353_17230: AO353_17230 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) (from data), at 3,809,838 to 3,810,638 _17230
Group Condition AO353_17210 AO353_17215 AO353_17220 AO353_17225 AO353_17230
carbon source L-Tryptophan (C) +0.3 -0.0 -0.8 -0.2 -3.0
nitrogen source L-Tryptophan (N) -0.0 -0.0 -0.3 -0.4 -1.0
carbon source L-Valine (C) -0.4 -0.0 -0.1 -0.2 -0.3
stress Sisomicin 0.008 mg/ml -0.6 -0.3 -0.1 -0.1 +0.2
stress Cisplatin 0.006 mg/ml -0.3 -0.7 -0.0 -0.3 +0.5
stress Lomefloxacin 0.001 mM +0.0 -0.1 -0.3 -0.1 -0.4
denitrifying Nitrate reduction, replicate B -0.2 -0.2 +0.1 -0.4 +0.0
carbon source L-Phenylalanine (C) -0.2 +0.4 +0.0 -0.3 -0.5
carbon source L-Ornithine +0.0 -0.8 +0.0 +0.2 -0.0
stress Doxycycline hyclate 0.0006 mg/ml +0.2 -1.2 +0.1 +0.2 +0.2
carbon source Ethanol (C) -0.3 +0.1 +0.1 -0.3 -0.1
survival 4C survival; 360 hrs +0.3 -0.4 -0.2 +0.1 -0.1
motility outer cut, LB soft agar motility assay -0.0 +0.3 +0.1 -0.7 -0.0
stress acetate 34 mM -0.2 +0.1 -0.0 -0.3 +0.3
stress Cisplatin 0.006 mg/ml +0.4 -0.2 -0.0 -0.5 +0.1
pH Minimal media pH9 -0.0 -0.5 -0.1 +0.1 +0.4
carbon source L-Citrulline -0.3 +0.6 -0.1 -0.2 -0.1
nitrogen source L-Lysine (N) -0.1 +0.3 -0.1 -0.3 +0.1
carbon source L-Ornithine -0.2 -0.3 +0.0 +0.6 -0.1
carbon source L-Aspartic Acid (C) +0.0 +0.3 +0.2 -0.1 -0.3
survival 4C survival; 672 hrs +0.5 -0.2 -0.1 +0.2 -0.0
carbon source L-Citrulline +0.1 +0.4 -0.0 +0.5 -0.6
carbon source L-Citrulline +0.6 -0.3 +0.1 +0.0 +0.1
motility outer cut, LB soft agar motility assay +0.3 +0.2 -0.2 +0.1 +0.4
carbon source D-Glucose -0.1 +0.4 +0.2 +0.2 +0.1
carbon source D-Glucose +0.4 -0.2 +0.2 +0.5 +0.0
nitrogen source L-Arginine (N) +0.3 +0.5 +0.0 +0.1 +0.1
carbon source acetate (C) +0.2 +0.3 +0.1 +0.3 +0.3
stress Benzalkonium Chloride 0.012 mg/ml +0.5 +0.1 +0.1 +0.2 +0.3
anaerobic Anaerobic, acetate donor; nitrate and iron +0.5 +0.6 +0.3 +0.2 +0.0
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