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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_16950 and AO353_16955 are separated by 13 nucleotides
AO353_16955 and AO353_16960 are separated by 27 nucleotides
AO353_16960 and AO353_16965 overlap by 4 nucleotides
AO353_16965 and AO353_16970 are separated by 58 nucleotides
AO353_16950: AO353_16950 - amino acid transporter, at 3,749,517 to 3,750,281
_16950
AO353_16955: AO353_16955 - succinylglutamate desuccinylase, at 3,750,295 to 3,751,413
_16955
AO353_16960: AO353_16960 - amino acid ABC transporter permease, at 3,751,441 to 3,752,151
_16960
AO353_16965: AO353_16965 - amino acid ABC transporter permease, at 3,752,148 to 3,752,876
_16965
AO353_16970: AO353_16970 - ABC transporter substrate-binding protein, at 3,752,935 to 3,753,714
_16970
Group
Condition
AO353
_16950
AO353
_16955
AO353
_16960
AO353
_16965
AO353
_16970
nitrogen source
L-Lysine (N)
N.D.
-0.8
-1.3
-1.1
-1.2
nitrogen source
L-Lysine (N)
N.D.
-0.3
-1.2
-1.1
-1.1
nitrogen source
Uridine (N)
N.D.
-0.8
-0.3
-0.3
-0.5
anaerobic
Anaerobic, acetate donor; nitrate and iron
N.D.
-1.5
+0.0
-0.3
+0.0
nitrogen source
NAG (N)
N.D.
-0.7
-0.2
-0.0
-0.4
motility
outer cut, LB soft agar motility assay
N.D.
-1.2
+0.1
-0.1
-0.1
carbon source
propionate (C)
N.D.
-1.0
+0.1
-0.0
-0.3
nitrogen source
Parabanic Acid (N)
N.D.
-0.6
-0.2
+0.0
-0.3
nitrogen source
Thymine (N)
N.D.
-0.3
-0.2
-0.2
-0.4
stress
Paraquat dichloride 0.032 mg/mL
N.D.
-1.2
+0.2
-0.0
+0.1
motility
outer cut, LB soft agar motility assay
N.D.
-0.6
-0.3
+0.2
+0.0
stress
Nickel (II) chloride 0.625 mM
N.D.
-0.5
+0.1
+0.2
-0.4
nitrogen source
L-Threonine (N)
N.D.
-0.5
-0.2
+0.2
+0.2
nitrogen source
L-Arginine (N)
N.D.
+0.4
-0.3
-0.0
-0.3
stress
Doxycycline hyclate 0.0006 mg/ml
N.D.
+0.6
+0.4
-0.8
-0.4
motility
outer cut, LB soft agar motility assay
N.D.
+0.2
+0.2
+0.1
-0.5
carbon source
L-Ornithine
N.D.
-0.4
-0.3
+0.6
+0.1
carbon source
Ethanol (C)
N.D.
+0.3
+0.2
-0.5
+0.3
stress
acetate 17 mM
N.D.
+0.4
+0.1
+0.2
-0.3
carbon source
acetate (C)
N.D.
+0.3
+0.2
+0.5
-0.1
pH
Minimal media pH9
N.D.
-0.1
-0.1
+0.6
+0.5
carbon source
L-Ornithine
N.D.
-0.2
+0.3
+0.7
+0.2
carbon source
L-Citrulline (C)
N.D.
+0.1
+0.2
+0.4
+0.3
stress
Cisplatin 0.006 mg/ml
N.D.
+0.5
+0.4
+0.5
-0.2
stress
Cisplatin 0.006 mg/ml
N.D.
+0.3
+0.2
+0.1
+0.6
carbon source
L-Citrulline
N.D.
+0.1
+0.6
+0.4
+0.7
carbon source
L-Citrulline
N.D.
+0.3
+0.4
+0.7
+0.6
carbon source
L-Citrulline
N.D.
-0.1
+0.7
+0.7
+0.8
carbon source
L-Citrulline
N.D.
+0.3
+0.6
+0.7
+0.8
anaerobic
N1B4wt with N2E3m denitrifying replicate C
N.D.
+0.7
+0.8
+0.4
+0.3
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