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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_16585 and AO353_16590 are separated by 42 nucleotides
AO353_16590 and AO353_16595 overlap by 4 nucleotides
AO353_16595 and AO353_16600 overlap by 13 nucleotides
AO353_16600 and AO353_16605 overlap by 1 nucleotides
AO353_16585: AO353_16585 - S-formylglutathione hydrolase, at 3,684,915 to 3,685,760
_16585
AO353_16590: AO353_16590 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 3,685,803 to 3,686,276
_16590
AO353_16595: AO353_16595 - pseudouridine synthase, at 3,686,273 to 3,687,331
_16595
AO353_16600: AO353_16600 - stationary phase survival protein SurE, at 3,687,319 to 3,688,068
_16600
AO353_16605: AO353_16605 - protein-L-isoaspartate O-methyltransferase, at 3,688,068 to 3,688,745
_16605
Group
Condition
AO353
_16585
AO353
_16590
AO353
_16595
AO353
_16600
AO353
_16605
stress
methylglyoxal 0.009 vol%
-2.7
N.D.
-0.5
-1.0
-1.4
stress
methylglyoxal 0.008 vol%
-2.8
N.D.
-0.0
-0.9
-0.5
stress
Furfuryl Alcohol 0.25 vol%
-2.5
N.D.
-0.2
-0.2
-0.3
carbon source
propionate (C)
-0.5
N.D.
-0.3
-0.6
-1.2
carbon source
Carnitine Hydrochloride (C)
-0.4
N.D.
-0.4
-0.6
-1.1
carbon source
propionate (C)
-0.2
N.D.
-0.4
-0.9
-1.0
survival
4C survival; 672 hrs
-0.3
N.D.
-0.5
-0.8
-0.8
carbon source
L-Phenylalanine (C)
-0.4
N.D.
-0.1
-1.2
-0.6
carbon source
L-Ornithine
-0.2
N.D.
-0.3
-0.6
-1.2
stress
Lomefloxacin 0.001 mM
+0.1
N.D.
+0.0
-1.4
-1.0
carbon source
Trisodium citrate (C)
-0.3
N.D.
-0.5
-0.4
-1.0
carbon source
Putrescine (C)
+0.1
N.D.
-0.6
-0.9
-0.7
stress
Tetracycline 0.0016 mg/ml
-0.1
N.D.
+0.0
-1.2
-0.8
carbon source
D-Lactate (C)
-0.1
N.D.
-0.4
-0.7
-0.8
carbon source
L-Citrulline (C)
-0.0
N.D.
-0.3
-0.6
-1.0
carbon source
Trisodium citrate (C)
-0.2
N.D.
-0.3
-0.6
-0.9
carbon source
L-Valine (C)
-0.0
N.D.
-0.5
-0.6
-0.8
stress
Cisplatin 0.006 mg/ml
-0.0
N.D.
-0.2
-0.8
-0.9
stress
Rifampicin 0.005 mg/ml
+0.2
N.D.
+0.1
-0.8
-1.4
motility
outer cut, LB soft agar motility assay
-0.2
N.D.
+0.3
-1.1
-0.8
stress
Doxycycline hyclate 0.0006 mg/ml
-0.1
N.D.
-0.1
-0.8
-0.8
carbon source
Tween 20 (C)
-0.0
N.D.
-0.2
-0.7
-1.0
carbon source
L-Citrulline
-0.3
N.D.
+0.0
-0.8
-0.7
carbon source
L-Asparagine (C)
+0.1
N.D.
-0.4
-0.6
-0.7
stress
Cisplatin 0.006 mg/ml
-0.4
N.D.
+0.5
-0.8
-0.8
carbon source
L-Citrulline
+0.3
N.D.
-0.1
-0.8
-0.7
motility
outer cut, LB soft agar motility assay
-0.4
N.D.
+0.7
-1.0
-0.5
stress
Sisomicin 0.008 mg/ml
+0.2
N.D.
+0.3
+0.7
+1.1
stress
A22 0.002 mg/ml
+0.1
N.D.
+0.4
+1.1
+0.9
stress
A22 0.01 mg/ml
+0.2
N.D.
+1.1
+1.9
+2.0
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