Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_16555 and AO353_16560 are separated by 163 nucleotidesAO353_16560 and AO353_16565 are separated by 333 nucleotidesAO353_16565 and AO353_16570 overlap by 4 nucleotidesAO353_16570 and AO353_16575 are separated by 621 nucleotides AO353_16555: AO353_16555 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 3,678,555 to 3,679,400 _16555 AO353_16560: AO353_16560 - enolase, at 3,679,564 to 3,680,853 _16560 AO353_16565: AO353_16565 - cell division protein FtsB, at 3,681,187 to 3,681,465 _16565 AO353_16570: AO353_16570 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,681,462 to 3,682,169 _16570 AO353_16575: AO353_16575 - LysR family transcriptional regulator, at 3,682,791 to 3,683,687 _16575
Group Condition AO353_16555 AO353_16560 AO353_16565 AO353_16570 AO353_16575
stress Furfuryl Alcohol 0.25 vol% N.D. N.D. N.D. N.D. -0.8
stress methylglyoxal 0.009 vol% N.D. N.D. N.D. N.D. -0.8
stress methylglyoxal 0.008 vol% N.D. N.D. N.D. N.D. -0.8
nitrogen source Carnitine Hydrochloride (N) N.D. N.D. N.D. N.D. -0.5
stress Fusidic 0.125 mg/ml N.D. N.D. N.D. N.D. -0.4
carbon source Carnitine Hydrochloride (C) N.D. N.D. N.D. N.D. -0.4
carbon source L-Serine (C) N.D. N.D. N.D. N.D. -0.3
stress Furfuryl Alcohol 0.125 vol% N.D. N.D. N.D. N.D. -0.3
stress Cisplatin 0.006 mg/ml N.D. N.D. N.D. N.D. -0.3
anaerobic Anaerobic, acetate donor; nitrate and iron N.D. N.D. N.D. N.D. -0.2
stress Sisomicin 0.008 mg/ml N.D. N.D. N.D. N.D. -0.2
carbon source L-Lactate (C) N.D. N.D. N.D. N.D. -0.2
carbon source L-Isoleucine (C) N.D. N.D. N.D. N.D. -0.2
stress Spectinomycin 0.2 mg/ml N.D. N.D. N.D. N.D. -0.2
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. N.D. -0.2
carbon source Glycerol (C) N.D. N.D. N.D. N.D. -0.2
anaerobic N1B4wt with N2E3m denitrifying replicate C N.D. N.D. N.D. N.D. +0.2
carbon source D-Gluconic Acid (C) N.D. N.D. N.D. N.D. +0.2
carbon source D-Ribose (C) N.D. N.D. N.D. N.D. +0.2
carbon source L-Citrulline N.D. N.D. N.D. N.D. +0.2
carbon source Uridine (C) N.D. N.D. N.D. N.D. +0.2
pH Minimal media pH6 N.D. N.D. N.D. N.D. +0.2
stress sodium fluoride 6.25 mM N.D. N.D. N.D. N.D. +0.2
survival 4C survival; 672 hrs N.D. N.D. N.D. N.D. +0.2
carbon source D-Lactate (C) N.D. N.D. N.D. N.D. +0.2
stress Benzalkonium Chloride 0.012 mg/ml N.D. N.D. N.D. N.D. +0.2
nitrogen source L-Arginine (N) N.D. N.D. N.D. N.D. +0.2
carbon source L-Citrulline N.D. N.D. N.D. N.D. +0.3
carbon source acetate (C) N.D. N.D. N.D. N.D. +0.3
pH Minimal media pH9 N.D. N.D. N.D. N.D. +0.4
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