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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_16405 and AO353_16410 overlap by 1 nucleotides
AO353_16410 and AO353_16415 are separated by 196 nucleotides
AO353_16415 and AO353_16420 are separated by 262 nucleotides
AO353_16420 and AO353_16425 are separated by 125 nucleotides
AO353_16405: AO353_16405 - SAM-dependent methyltransferase, at 3,641,268 to 3,642,077
_16405
AO353_16410: AO353_16410 - succinyl-diaminopimelate desuccinylase, at 3,642,077 to 3,643,228
_16410
AO353_16415: AO353_16415 - beta-(1-3)-glucosyl transferase, at 3,643,425 to 3,646,016
_16415
AO353_16420: AO353_16420 - thiamine biosynthesis protein ThiF, at 3,646,279 to 3,647,097
_16420
AO353_16425: AO353_16425 - Fe-S metabolism protein SufE, at 3,647,223 to 3,647,627
_16425
Group
Condition
AO353
_16405
AO353
_16410
AO353
_16415
AO353
_16420
AO353
_16425
carbon source
L-Tryptophan (C)
+0.3
N.D.
+0.1
-0.4
-3.2
carbon source
L-Leucine (C)
-0.1
N.D.
+0.1
-0.0
-2.9
stress
copper (II) chloride 2.5 mM
-0.3
N.D.
-0.5
+0.2
-2.1
stress
methylglyoxal 0.009 vol%
+0.1
N.D.
+1.1
-1.4
-2.5
carbon source
D-Glucose
-0.3
N.D.
+0.1
-0.6
-1.9
carbon source
L-Citrulline
+0.0
N.D.
+0.1
+0.4
-3.0
pH
Minimal media pH7
+0.1
N.D.
-0.1
-0.0
-2.4
motility
outer cut, LB soft agar motility assay
-0.3
N.D.
-2.3
-0.4
+0.6
stress
Benzalkonium Chloride 0.012 mg/ml
+0.0
N.D.
+0.7
+0.3
-3.2
survival
4C survival; 672 hrs
-0.0
N.D.
-1.4
+0.1
-0.7
carbon source
L-Arginine (C)
-0.5
N.D.
+0.0
-0.1
-1.5
motility
outer cut, LB soft agar motility assay
-0.6
N.D.
-1.8
+0.2
+0.2
stress
Cobalt chloride 0.16 mM
-0.0
N.D.
-0.2
-0.4
-1.4
nitrogen source
Thymine (N)
+0.0
N.D.
+0.1
-0.1
-1.8
motility
outer cut, LB soft agar motility assay
+0.3
N.D.
-1.5
-0.5
+0.2
carbon source
Trisodium citrate (C)
+0.2
N.D.
+0.1
-0.2
-1.6
pH
Minimal media pH9
-0.1
N.D.
-0.1
+0.5
-1.4
stress
Cisplatin 0.006 mg/ml
+0.5
N.D.
+0.8
-0.5
-1.8
carbon source
L-Ornithine
+0.0
N.D.
-0.2
+0.7
-1.5
nitrogen source
Parabanic Acid (N)
+0.2
N.D.
+0.3
-0.7
-0.8
stress
Cisplatin 0.006 mg/ml
-0.2
N.D.
+0.6
-1.4
+0.1
stress
copper (II) chloride 2.5 mM
-0.2
N.D.
-1.1
+0.2
+1.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.4
N.D.
+0.3
+0.7
-0.5
stress
Tetracycline 0.0016 mg/ml
-0.3
N.D.
+0.5
+0.6
+1.0
stress
nitrate 200 mM
-0.2
N.D.
+0.3
+0.3
+1.3
stress
Sisomicin 0.008 mg/ml
-0.4
N.D.
+0.1
+0.3
+1.6
carbon source
L-Ornithine
+0.3
N.D.
-0.2
+0.5
+1.5
carbon source
L-Citrulline
-0.1
N.D.
+0.3
-0.1
+2.0
stress
Doxycycline hyclate 0.0006 mg/ml
-0.0
N.D.
+1.1
+0.9
+0.3
stress
Rifampicin 0.005 mg/ml
+0.1
N.D.
+0.8
+0.5
+1.0
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