Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_16405 and AO353_16410 overlap by 1 nucleotidesAO353_16410 and AO353_16415 are separated by 196 nucleotidesAO353_16415 and AO353_16420 are separated by 262 nucleotidesAO353_16420 and AO353_16425 are separated by 125 nucleotides AO353_16405: AO353_16405 - SAM-dependent methyltransferase, at 3,641,268 to 3,642,077 _16405 AO353_16410: AO353_16410 - succinyl-diaminopimelate desuccinylase, at 3,642,077 to 3,643,228 _16410 AO353_16415: AO353_16415 - beta-(1-3)-glucosyl transferase, at 3,643,425 to 3,646,016 _16415 AO353_16420: AO353_16420 - thiamine biosynthesis protein ThiF, at 3,646,279 to 3,647,097 _16420 AO353_16425: AO353_16425 - Fe-S metabolism protein SufE, at 3,647,223 to 3,647,627 _16425
Group Condition AO353_16405 AO353_16410 AO353_16415 AO353_16420 AO353_16425
carbon source L-Tryptophan (C) +0.3 N.D. +0.1 -0.4 -3.2
carbon source L-Leucine (C) -0.1 N.D. +0.1 -0.0 -2.9
stress copper (II) chloride 2.5 mM -0.3 N.D. -0.5 +0.2 -2.1
stress methylglyoxal 0.009 vol% +0.1 N.D. +1.1 -1.4 -2.5
carbon source D-Glucose -0.3 N.D. +0.1 -0.6 -1.9
carbon source L-Citrulline +0.0 N.D. +0.1 +0.4 -3.0
pH Minimal media pH7 +0.1 N.D. -0.1 -0.0 -2.4
motility outer cut, LB soft agar motility assay -0.3 N.D. -2.3 -0.4 +0.6
stress Benzalkonium Chloride 0.012 mg/ml +0.0 N.D. +0.7 +0.3 -3.2
survival 4C survival; 672 hrs -0.0 N.D. -1.4 +0.1 -0.7
carbon source L-Arginine (C) -0.5 N.D. +0.0 -0.1 -1.5
motility outer cut, LB soft agar motility assay -0.6 N.D. -1.8 +0.2 +0.2
stress Cobalt chloride 0.16 mM -0.0 N.D. -0.2 -0.4 -1.4
nitrogen source Thymine (N) +0.0 N.D. +0.1 -0.1 -1.8
motility outer cut, LB soft agar motility assay +0.3 N.D. -1.5 -0.5 +0.2
carbon source Trisodium citrate (C) +0.2 N.D. +0.1 -0.2 -1.6
pH Minimal media pH9 -0.1 N.D. -0.1 +0.5 -1.4
stress Cisplatin 0.006 mg/ml +0.5 N.D. +0.8 -0.5 -1.8
carbon source L-Ornithine +0.0 N.D. -0.2 +0.7 -1.5
nitrogen source Parabanic Acid (N) +0.2 N.D. +0.3 -0.7 -0.8
stress Cisplatin 0.006 mg/ml -0.2 N.D. +0.6 -1.4 +0.1
stress copper (II) chloride 2.5 mM -0.2 N.D. -1.1 +0.2 +1.1
anaerobic Anaerobic, acetate donor; nitrate and iron +0.4 N.D. +0.3 +0.7 -0.5
stress Tetracycline 0.0016 mg/ml -0.3 N.D. +0.5 +0.6 +1.0
stress nitrate 200 mM -0.2 N.D. +0.3 +0.3 +1.3
stress Sisomicin 0.008 mg/ml -0.4 N.D. +0.1 +0.3 +1.6
carbon source L-Ornithine +0.3 N.D. -0.2 +0.5 +1.5
carbon source L-Citrulline -0.1 N.D. +0.3 -0.1 +2.0
stress Doxycycline hyclate 0.0006 mg/ml -0.0 N.D. +1.1 +0.9 +0.3
stress Rifampicin 0.005 mg/ml +0.1 N.D. +0.8 +0.5 +1.0
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