Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_16115 and AO353_16120 are separated by 612 nucleotidesAO353_16120 and AO353_16125 are separated by 222 nucleotidesAO353_16125 and AO353_16130 are separated by 300 nucleotidesAO353_16130 and AO353_16135 overlap by 4 nucleotides AO353_16115: AO353_16115 - transposase, at 3,572,674 to 3,573,132 _16115 AO353_16120: AO353_16120 - D-alanine and L-alanine transporter (from data), at 3,573,745 to 3,575,163 _16120 AO353_16125: AO353_16125 - hypothetical protein, at 3,575,386 to 3,576,510 _16125 AO353_16130: AO353_16130 - hypothetical protein, at 3,576,811 to 3,577,185 _16130 AO353_16135: AO353_16135 - bactoprenol glucosyl transferase, at 3,577,182 to 3,578,120 _16135
Group Condition AO353_16115 AO353_16120 AO353_16125 AO353_16130 AO353_16135
carbon source D-Alanine (C) +0.1 -2.4 -0.0 -0.1 -0.0
anaerobic Anaerobic, acetate donor; nitrate and iron -0.6 -0.4 -0.1 +0.0 -0.2
nitrogen source D-Alanine (N) +0.3 -1.1 -0.1 -0.1 -0.1
stress Cisplatin 0.006 mg/ml -0.2 -0.4 -0.2 +0.2 -0.4
carbon source L-Alanine (C) +0.5 -1.5 +0.1 -0.1 +0.0
survival 4C survival; 672 hrs -0.1 +0.1 -0.9 +0.2 -0.1
stress Cisplatin 0.006 mg/ml +0.4 -0.1 -0.6 -0.0 -0.5
stress Spectinomycin 0.2 mg/ml +0.1 +0.0 -0.1 -0.3 -0.4
stress Doxycycline hyclate 0.0002 mg/ml +0.0 -0.1 +0.3 -0.6 -0.4
carbon source L-Valine (C) +0.1 -0.6 -0.3 +0.1 -0.0
survival 4C survival; 360 hrs +0.1 +0.0 -0.6 -0.1 -0.1
motility outer cut, LB soft agar motility assay +0.4 -0.1 -0.7 -0.0 -0.1
stress Nickel (II) chloride 0.625 mM -0.1 -0.1 -0.6 +0.3 -0.0
stress 1-ethyl-3-methylimidazolium lysine 10.5 mM +0.1 +0.2 -0.5 -0.1 -0.1
carbon source D-Glucose -0.4 +0.2 +0.3 -0.1 -0.3
stress Lomefloxacin 0.001 mM +0.5 -0.1 -0.0 -0.2 -0.5
carbon source L-Malic (C) +0.3 +0.1 -0.3 -0.1 -0.2
survival 4C survival; 192 hrs +0.2 -0.2 -0.4 +0.2 -0.0
stress Rifampicin 0.005 mg/ml +0.1 +0.0 +0.4 -0.3 -0.2
stress Tetracycline 0.0016 mg/ml +0.2 -0.0 +0.4 -0.4 -0.2
stress Doxycycline hyclate 0.0006 mg/ml +0.4 -0.3 +0.5 -0.2 -0.3
stress Fusidic 0.125 mg/ml -0.3 +0.1 +0.8 -0.4 -0.2
stress copper (II) chloride 2.5 mM +0.3 +0.1 +0.1 -0.2 -0.2
motility outer cut, LB soft agar motility assay +0.5 +0.0 -0.6 +0.0 +0.1
stress Choline Lysine 12 mM +0.4 -0.1 -0.4 +0.1 +0.2
nitrogen source L-Serine (N) -0.2 +0.5 +0.0 -0.3 +0.1
motility outer cut, LB soft agar motility assay +0.4 +0.3 -0.8 +0.1 +0.2
stress Sisomicin 0.008 mg/ml +0.2 +0.2 +1.0 -0.8 -0.5
denitrifying Nitrate reduction, replicate B -0.2 +0.4 -0.1 +0.2 -0.0
denitrifying Nitrate reduction, replicate C +0.0 +0.3 +0.1 +0.6 +0.2
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