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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_15565 and AO353_15570 are separated by 184 nucleotides
AO353_15570 and AO353_15575 are separated by 101 nucleotides
AO353_15575 and AO353_15580 overlap by 8 nucleotides
AO353_15580 and AO353_15585 are separated by 28 nucleotides
AO353_15565: AO353_15565 - hypothetical protein, at 3,445,141 to 3,445,944
_15565
AO353_15570: AO353_15570 - amino acid dehydrogenase, at 3,446,129 to 3,447,148
_15570
AO353_15575: AO353_15575 - pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha, at 3,447,250 to 3,448,347
_15575
AO353_15580: AO353_15580 - 2-oxoisovalerate dehydrogenase, at 3,448,340 to 3,449,326
_15580
AO353_15585: AO353_15585 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 3,449,355 to 3,450,464
_15585
Group
Condition
AO353
_15565
AO353
_15570
AO353
_15575
AO353
_15580
AO353
_15585
stress
Cisplatin 0.006 mg/ml
-0.6
-0.3
-0.0
-0.6
-0.7
carbon source
L-Citrulline
-0.4
-0.2
-0.1
-0.4
-1.0
stress
Cisplatin 0.006 mg/ml
-1.0
-0.3
+0.0
-0.3
-0.5
carbon source
L-Isoleucine (C)
-0.3
-0.5
+0.1
+0.2
-1.1
stress
Paraquat dichloride 0.032 mg/mL
-0.5
-0.1
-0.2
+0.0
-0.8
carbon source
L-Citrulline (C)
-0.1
-0.1
-0.3
-0.7
-0.4
stress
Thallium(I) acetate 0.016 mg/ml
-0.1
+0.1
+0.1
-0.9
-0.7
carbon source
L-Ornithine
-0.3
+0.0
-0.6
+0.5
-1.0
denitrifying
Nitrate reduction, replicate B
-1.1
-0.2
+0.2
-0.1
-0.2
stress
Spectinomycin 0.2 mg/ml
-0.3
+0.0
-0.0
-0.3
-0.7
stress
Lomefloxacin 0.001 mM
-0.7
-0.0
+0.1
-0.3
-0.2
carbon source
L-Phenylalanine (C)
+0.2
-0.1
+0.1
-0.5
-0.5
stress
Doxycycline hyclate 0.0006 mg/ml
-0.3
-0.3
+0.7
+0.1
-0.8
carbon source
Ethanol (C)
-0.7
+0.1
-0.0
-0.2
+0.3
stress
acetate 17 mM
+0.2
-0.1
+0.1
-0.4
-0.5
carbon source
propionate (C)
-0.4
-0.2
-0.4
+0.6
-0.2
nitrogen source
Uridine (N)
+0.4
-0.3
-0.1
-0.1
-0.5
lb
LB
+0.4
-0.1
+0.2
-0.4
-0.5
stress
methylglyoxal 0.009 vol%
-0.1
-0.2
-0.1
+0.5
-0.5
nitrogen source
Gly-Glu (N)
+0.6
-0.2
-0.1
-0.2
-0.4
motility
outer cut, LB soft agar motility assay
+0.5
+0.1
+0.2
-0.2
-0.6
carbon source
D-Fructose (C)
-0.0
+0.2
+0.2
+0.3
-0.6
motility
outer cut, LB soft agar motility assay
-0.4
-0.4
+0.1
+0.1
+0.8
carbon source
L-Ornithine
+0.2
+0.0
+0.0
+0.6
-0.5
motility
outer cut, LB soft agar motility assay
+0.9
+0.2
-0.5
-0.4
+0.5
temperature
LB 30C
+0.5
+0.2
+0.2
-0.3
+0.2
carbon source
acetate (C)
-0.2
+0.2
-0.0
+0.6
+0.5
nitrogen source
nitrite (N)
+0.5
+0.0
+0.3
+0.1
+0.4
pH
Minimal media pH9
+0.4
+0.2
+0.1
+0.3
+0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.2
+0.5
+0.4
+0.8
-0.1
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