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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_15075 and AO353_15080 are separated by 23 nucleotides
AO353_15080 and AO353_15095 are separated by 1121 nucleotides
AO353_15095 and AO353_15100 are separated by 448 nucleotides
AO353_15100 and AO353_15105 are separated by 315 nucleotides
AO353_15075: AO353_15075 - cysteine methyltransferase, at 3,335,828 to 3,336,328
_15075
AO353_15080: AO353_15080 - hypothetical protein, at 3,336,352 to 3,336,612
_15080
AO353_15095: AO353_15095 - hypothetical protein, at 3,337,734 to 3,338,054
_15095
AO353_15100: AO353_15100 - GNAT family acetyltransferase, at 3,338,503 to 3,338,979
_15100
AO353_15105: AO353_15105 - porin, at 3,339,295 to 3,340,653
_15105
Group
Condition
AO353
_15075
AO353
_15080
AO353
_15095
AO353
_15100
AO353
_15105
carbon source
L-Leucine (C)
-1.0
+0.2
-0.2
-0.2
-0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.0
+0.0
-0.8
-0.3
+0.1
stress
Cisplatin 0.006 mg/ml
-0.6
+0.1
+0.3
-0.6
-0.1
stress
Sisomicin 0.008 mg/ml
-0.7
-0.0
-0.0
-0.3
+0.3
carbon source
L-Alanine (C)
-0.2
-0.3
+0.1
-0.5
+0.1
stress
Doxycycline hyclate 0.0002 mg/ml
-0.3
+0.1
-0.1
-0.3
+0.1
carbon source
L-Ornithine
-0.0
+0.1
+0.2
-0.5
-0.1
carbon source
L-Aspartic Acid (C)
-0.6
+0.2
+0.0
+0.1
-0.0
carbon source
L-Citrulline
-0.8
-0.0
+0.3
+0.2
+0.1
pH
Minimal media pH9
-0.3
+0.4
-0.0
-0.5
+0.3
stress
Cisplatin 0.006 mg/ml
-0.4
+0.1
+0.3
-0.4
+0.2
nitrogen source
Inosine (N)
-0.3
-0.2
+0.3
+0.2
-0.0
stress
methylglyoxal 0.009 vol%
-0.4
-0.1
-0.0
+0.3
+0.2
carbon source
L-Citrulline
-0.1
+0.1
+0.1
-0.4
+0.3
denitrifying
Nitrate reduction, replicate C
-0.3
+0.3
-0.2
+0.2
+0.1
nitrogen source
Parabanic Acid (N)
-0.2
+0.2
-0.2
+0.4
-0.0
carbon source
a-Ketoglutaric (C)
-0.4
+0.2
+0.2
+0.0
+0.2
carbon source
L-Serine (C)
-0.2
+0.3
-0.2
-0.1
+0.3
radiation
Ionizing radiation, 126.196 Gy,
+0.1
-0.2
+0.2
+0.2
-0.2
survival
4C survival; 360 hrs
-0.3
+0.0
+0.3
+0.2
-0.0
carbon source
D-Alanine (C)
+0.6
-0.1
+0.2
-0.1
-0.1
stress
Lomefloxacin 0.001 mM
-0.3
-0.1
+0.4
+0.4
+0.1
carbon source
D-Glucose
+0.1
+0.1
+0.2
+0.4
-0.2
stress
Tetracycline 0.0016 mg/ml
+0.4
+0.2
+0.2
-0.2
+0.0
motility
outer cut, LB soft agar motility assay
+0.0
+0.2
+0.4
+0.3
-0.2
carbon source
L-Ornithine
-0.2
+0.3
+0.2
+0.4
+0.0
stress
Fusidic 0.125 mg/ml
+0.4
-0.1
+0.4
+0.1
+0.1
stress
A22 0.002 mg/ml
+0.5
-0.1
+0.3
+0.2
+0.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.7
-0.0
+0.5
+0.1
-0.2
carbon source
acetate (C)
+0.0
+0.3
+0.0
+0.6
+0.6
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