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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_14985 and AO353_14990 are separated by 225 nucleotides
AO353_14990 and AO353_14995 are separated by 251 nucleotides
AO353_14995 and AO353_15000 are separated by 33 nucleotides
AO353_15000 and AO353_15005 are separated by 287 nucleotides
AO353_14985: AO353_14985 - peptidase M48, at 3,312,534 to 3,313,352
_14985
AO353_14990: AO353_14990 - hypothetical protein, at 3,313,578 to 3,314,162
_14990
AO353_14995: AO353_14995 - 16S rRNA methyltransferase, at 3,314,414 to 3,315,412
_14995
AO353_15000: AO353_15000 - glycerate dehydrogenase, at 3,315,446 to 3,316,411
_15000
AO353_15005: AO353_15005 - lysine transporter LysE, at 3,316,699 to 3,317,328
_15005
Group
Condition
AO353
_14985
AO353
_14990
AO353
_14995
AO353
_15000
AO353
_15005
carbon source
D-Fructose (C)
+0.0
-3.9
-0.6
-0.9
-0.2
carbon source
L-Phenylalanine (C)
+0.1
-3.4
-0.3
-1.1
+0.1
stress
Polymyxin B sulfate 4 mg/ml
+0.1
-4.1
+0.3
-0.2
-0.4
pH
Minimal media pH9
+0.2
-4.2
+0.3
-0.4
-0.2
carbon source
Trisodium citrate (C)
-0.2
-2.9
-0.5
+0.0
-0.7
carbon source
Trisodium citrate (C)
-0.3
-3.1
-0.6
+0.2
-0.3
pH
Minimal media pH9
-0.2
-4.1
+0.6
-0.4
+0.2
carbon source
propionate (C)
+0.1
-3.6
+0.3
-0.4
+0.1
motility
outer cut, LB soft agar motility assay
-0.2
-1.3
-0.3
-0.9
+0.2
stress
Cobalt chloride 0.32 mM
-0.1
-1.1
-0.5
-0.6
-0.1
stress
methylglyoxal 0.009 vol%
+0.0
-0.3
-0.8
-0.5
-0.6
stress
Fusidic 0.125 mg/ml
-0.0
-2.0
+0.2
+0.1
-0.2
stress
D-Cycloserine 0.1 mg/ml
+0.1
-1.3
-0.5
+0.3
-0.4
carbon source
pyruvate (C)
+0.1
-1.9
-0.1
+0.2
-0.1
carbon source
L-Tryptophan (C)
+0.2
-1.8
-0.4
+0.6
-0.2
carbon source
Glycerol (C)
-0.0
-2.2
-0.0
+0.3
+0.2
carbon source
Glycerol (C)
+0.1
-1.2
+0.3
-0.3
-0.5
carbon source
L-Arginine (C)
-0.1
-1.8
-0.0
+0.1
+0.2
carbon source
L-Citrulline
-0.4
-0.7
+0.4
-0.6
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.4
+0.1
-0.4
-1.3
+0.5
nitrogen source
L-Glutamine (N)
+0.2
-1.6
+0.4
+0.4
-0.7
pH
Minimal media pH6
-0.1
-1.3
-0.3
+0.5
+0.1
carbon source
L-Citrulline
-0.1
-2.5
+0.8
+0.9
-0.1
stress
copper (II) chloride 2.5 mM
-0.1
-1.4
+0.3
+0.2
+0.4
stress
Rifampicin 0.005 mg/ml
-0.0
-1.9
+0.7
+0.5
+0.3
carbon source
L-Valine (C)
-0.0
+0.6
-0.5
-0.8
+0.5
stress
Sisomicin 0.008 mg/ml
+0.3
-0.9
+2.9
-0.7
-0.5
carbon source
D-Glucose
+0.1
+1.2
-0.6
+0.4
+0.2
motility
outer cut, LB soft agar motility assay
+0.4
+1.4
+0.2
+0.3
-0.5
stress
Doxycycline hyclate 0.0006 mg/ml
+0.0
+1.3
+0.5
+0.7
+0.4
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