Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_14665 and AO353_14670 are separated by 46 nucleotidesAO353_14670 and AO353_14675 are separated by 190 nucleotidesAO353_14675 and AO353_14680 are separated by 123 nucleotidesAO353_14680 and AO353_14685 are separated by 116 nucleotides AO353_14665: AO353_14665 - alpha/beta hydrolase, at 3,248,355 to 3,249,188 _14665 AO353_14670: AO353_14670 - gamma-glutamyltransferase, at 3,249,235 to 3,251,079 _14670 AO353_14675: AO353_14675 - hypothetical protein, at 3,251,270 to 3,252,373 _14675 AO353_14680: AO353_14680 - tRNA-Met, at 3,252,497 to 3,252,573 _14680 AO353_14685: AO353_14685 - chemotaxis protein, at 3,252,690 to 3,254,747 _14685
Group Condition AO353_14665 AO353_14670 AO353_14675 AO353_14680 AO353_14685
stress Cisplatin 0.006 mg/ml -0.2 +0.3 -0.8 N.D. -0.7
survival 4C survival; 360 hrs -1.1 -0.2 -0.0 N.D. +0.1
carbon source L-Ornithine -0.6 +0.0 -0.4 N.D. -0.1
carbon source L-Citrulline +0.2 -0.0 -0.1 N.D. -1.1
carbon source L-Leucine (C) -0.2 -0.1 -0.1 N.D. -0.5
anaerobic Anaerobic, acetate donor; nitrate and iron -0.3 -0.0 +0.1 N.D. -0.8
temperature LB 20C -0.5 -0.1 -0.1 N.D. -0.2
carbon source D-Glucose -0.6 -0.2 -0.1 N.D. +0.2
carbon source L-Ornithine -0.0 -0.0 -0.4 N.D. -0.3
carbon source L-Valine (C) -0.1 +0.0 -0.2 N.D. -0.4
anaerobic N1B4wt with N2E3m denitrifying replicate C +0.1 -0.2 -0.3 N.D. -0.3
temperature LB 30C -0.5 +0.1 +0.0 N.D. -0.2
pH Minimal media pH9 -0.2 -0.4 +0.2 N.D. -0.3
pH Minimal media pH7 -0.4 -0.2 -0.1 N.D. +0.2
carbon source D-Trehalose (C) -0.4 -0.2 -0.1 N.D. +0.3
stress 1-ethyl-3-methylimidazolium acetate 15 mM -0.2 +0.2 +0.1 N.D. -0.4
stress Lomefloxacin 0.001 mM -0.6 +0.2 +0.2 N.D. -0.1
stress copper (II) chloride 2.5 mM -0.5 +0.2 +0.1 N.D. +0.3
stress Rifampicin 0.005 mg/ml -0.4 +0.1 +0.3 N.D. +0.1
motility outer cut, LB soft agar motility assay +0.3 +0.4 -0.1 N.D. -0.5
stress 1-ethyl-3-methylimidazolium acetate 30 mM -0.2 +0.4 -0.2 N.D. +0.1
carbon source Ethanol (C) -0.5 +0.4 +0.1 N.D. +0.2
stress Cobalt chloride 0.32 mM -0.3 +0.2 -0.0 N.D. +0.3
carbon source D-Glucose +0.2 +0.3 +0.2 N.D. -0.4
denitrifying Nitrate reduction, replicate B +0.4 -0.1 +0.2 N.D. -0.2
stress Polymyxin B sulfate 4 mg/ml +0.4 +0.2 -0.1 N.D. -0.1
nitrogen source NAG (N) +0.2 +0.0 -0.2 N.D. +0.4
stress Cisplatin 0.006 mg/ml +0.2 +0.4 +0.3 N.D. -0.2
carbon source L-Glutamine (C) +0.3 -0.2 +0.4 N.D. +0.3
carbon source L-Phenylalanine (C) +0.4 +0.1 +0.1 N.D. +0.3
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