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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_14655 and AO353_14660 are separated by 3 nucleotides
AO353_14660 and AO353_14665 are separated by 297 nucleotides
AO353_14665 and AO353_14670 are separated by 46 nucleotides
AO353_14670 and AO353_14675 are separated by 190 nucleotides
AO353_14655: AO353_14655 - transcriptional regulator, at 3,247,380 to 3,247,751
_14655
AO353_14660: AO353_14660 - addiction module toxin RelE, at 3,247,755 to 3,248,057
_14660
AO353_14665: AO353_14665 - alpha/beta hydrolase, at 3,248,355 to 3,249,188
_14665
AO353_14670: AO353_14670 - gamma-glutamyltransferase, at 3,249,235 to 3,251,079
_14670
AO353_14675: AO353_14675 - hypothetical protein, at 3,251,270 to 3,252,373
_14675
Group
Condition
AO353
_14655
AO353
_14660
AO353
_14665
AO353
_14670
AO353
_14675
survival
4C survival; 360 hrs
-0.5
-0.2
-1.1
-0.2
-0.0
carbon source
L-Ornithine
-0.4
-0.2
-0.6
+0.0
-0.4
nitrogen source
Gly-Glu (N)
-0.9
-0.3
+0.1
-0.1
+0.0
carbon source
L-Valine (C)
-0.8
-0.0
-0.1
+0.0
-0.2
carbon source
L-Ornithine
-0.4
-0.2
-0.2
-0.2
-0.1
stress
acetate 68 mM
-0.4
+0.0
-0.4
-0.1
-0.2
carbon source
L-Citrulline
-0.8
-0.2
+0.2
-0.0
-0.1
carbon source
D-Glucose
+0.2
+0.0
-0.6
-0.2
-0.1
temperature
LB 20C
-0.2
+0.2
-0.5
-0.1
-0.1
motility
outer cut, LB soft agar motility assay
+0.2
-0.3
-0.5
-0.1
+0.0
stress
Cisplatin 0.006 mg/ml
-0.5
+0.5
-0.2
+0.3
-0.8
stress
Tetracycline 0.0008 mg/ml
-0.4
-0.2
-0.1
+0.2
+0.0
stress
copper (II) chloride 2.5 mM
-0.3
+0.1
-0.5
+0.2
+0.1
pH
Minimal media pH7
+0.0
+0.3
-0.4
-0.2
-0.1
nitrogen source
NAG (N)
-0.3
-0.2
+0.2
+0.0
-0.2
stress
Cobalt chloride 0.32 mM
-0.4
+0.3
-0.3
+0.2
-0.0
stress
Rifampicin 0.005 mg/ml
-0.0
-0.1
-0.4
+0.1
+0.3
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.2
+0.1
-0.2
+0.4
-0.2
pH
Minimal media pH9
+0.3
+0.0
-0.2
-0.4
+0.2
stress
Doxycycline hyclate 0.0006 mg/ml
-0.5
+0.2
+0.0
-0.0
+0.3
stress
Sisomicin 0.008 mg/ml
+0.6
+0.1
-0.3
-0.2
-0.1
carbon source
L-Glutamine (C)
-0.1
-0.1
+0.3
-0.2
+0.4
carbon source
Ethanol (C)
+0.1
+0.2
-0.5
+0.4
+0.1
stress
Doxycycline hyclate 0.0002 mg/ml
-0.4
+0.3
-0.0
+0.3
+0.1
stress
Lomefloxacin 0.001 mM
+0.6
+0.1
-0.6
+0.2
+0.2
carbon source
L-Serine (C)
+0.7
+0.2
-0.2
+0.0
+0.1
carbon source
acetate (C)
+0.4
+0.2
-0.2
+0.3
+0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.4
+0.5
-0.3
-0.0
+0.1
stress
Cisplatin 0.006 mg/ml
-0.4
+0.3
+0.2
+0.4
+0.3
motility
outer cut, LB soft agar motility assay
+0.4
-0.1
+0.3
+0.4
-0.1
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