Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_14200 and AO353_14205 overlap by 4 nucleotidesAO353_14205 and AO353_14210 overlap by 11 nucleotidesAO353_14210 and AO353_14215 are separated by 124 nucleotidesAO353_14215 and AO353_14220 are separated by 152 nucleotides AO353_14200: AO353_14200 - transcriptional regulator, at 3,153,128 to 3,154,474 _14200 AO353_14205: AO353_14205 - histidine kinase, at 3,154,471 to 3,156,060 _14205 AO353_14210: AO353_14210 - hypothetical protein, at 3,156,050 to 3,156,283 _14210 AO353_14215: AO353_14215 - DNA transporter, at 3,156,408 to 3,157,424 _14215 AO353_14220: AO353_14220 - ribosomal large subunit pseudouridine synthase D, at 3,157,577 to 3,158,539 _14220
Group Condition AO353_14200 AO353_14205 AO353_14210 AO353_14215 AO353_14220
survival 4C survival; 192 hrs -0.1 -0.1 -0.2 N.D. -4.1
stress Cobalt chloride 0.32 mM -0.1 +0.0 -0.1 N.D. -4.3
temperature LB 30C -0.0 +0.1 +0.1 N.D. -4.1
carbon source L-Phenylalanine (C) -0.0 -0.1 -0.7 N.D. -3.1
nitrogen source L-Glutamic (N) -0.1 -0.1 +0.0 N.D. -3.7
carbon source L-Isoleucine (C) -0.3 -0.2 -0.0 N.D. -3.2
nitrogen source L-Histidine (N) -0.1 -0.0 +0.0 N.D. -3.6
stress Cisplatin 0.006 mg/ml -0.3 -0.2 -0.2 N.D. -3.0
carbon source L-Alanine (C) +0.1 -0.2 -0.7 N.D. -2.8
carbon source D-Serine (C) -0.0 -0.0 -0.3 N.D. -3.2
stress D-Cycloserine 0.1 mg/ml +0.0 +0.2 -0.0 N.D. -3.8
nitrogen source L-Arginine (N) -0.0 -0.1 +0.1 N.D. -3.5
survival 4C survival; 360 hrs -0.0 +0.0 +0.3 N.D. -3.8
motility outer cut, LB soft agar motility assay -0.1 +0.3 -0.9 N.D. -2.8
stress Thallium(I) acetate 0.008 mg/ml +0.1 +0.1 +0.0 N.D. -3.4
stress Nickel (II) chloride 0.625 mM +0.1 +0.0 -0.1 N.D. -3.3
carbon source Tween 20 (C) -0.1 -0.1 +0.1 N.D. -3.1
pH Minimal media pH8 -0.1 +0.1 +0.0 N.D. -3.2
stress Cobalt chloride 0.16 mM +0.1 -0.2 +0.2 N.D. -3.2
carbon source Trisodium citrate (C) -0.1 +0.1 -0.3 N.D. -2.9
stress copper (II) chloride 2.5 mM +0.1 -0.1 -1.3 N.D. -1.8
motility outer cut, LB soft agar motility assay +0.1 +0.4 -0.3 N.D. -3.3
pH Minimal media pH6 -0.1 +0.1 -0.1 N.D. -3.0
nitrogen source Inosine (N) +0.1 -0.1 +0.1 N.D. -3.1
carbon source L-Valine (C) +0.2 +0.0 -0.1 N.D. -3.0
stress methylglyoxal 0.009 vol% +0.2 +0.0 +0.4 N.D. -3.3
carbon source L-Leucine (C) +0.1 +0.2 +0.1 N.D. -3.0
pH Minimal media pH9 -0.0 +0.2 +0.4 N.D. -2.8
stress Lomefloxacin 0.001 mM +0.1 +0.0 +0.1 N.D. +3.5
stress Sisomicin 0.008 mg/ml -0.1 +0.2 +1.0 N.D. +5.3
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