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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_13895 and AO353_13900 are separated by 368 nucleotides
AO353_13900 and AO353_13905 are separated by 55 nucleotides
AO353_13905 and AO353_13910 overlap by 4 nucleotides
AO353_13910 and AO353_13915 are separated by 215 nucleotides
AO353_13895: AO353_13895 - chemotaxis protein, at 3,086,694 to 3,088,640
_13895
AO353_13900: AO353_13900 - kinase, at 3,089,009 to 3,089,902
_13900
AO353_13905: AO353_13905 - tRNA (uracil-5-)-methyltransferase, at 3,089,958 to 3,091,037
_13905
AO353_13910: AO353_13910 - guanine permease, at 3,091,034 to 3,092,329
_13910
AO353_13915: AO353_13915 - hypothetical protein, at 3,092,545 to 3,093,003
_13915
Group
Condition
AO353
_13895
AO353
_13900
AO353
_13905
AO353
_13910
AO353
_13915
stress
Cobalt chloride 0.32 mM
+0.0
+0.0
-1.7
-0.8
-0.0
stress
Cobalt chloride 0.32 mM
-0.1
-0.1
-1.8
-0.4
-0.0
carbon source
L-Citrulline
+0.0
+0.0
-0.5
-0.6
-0.5
pH
Minimal media pH9
+0.2
+0.2
-1.1
-0.7
+0.1
motility
outer cut, LB soft agar motility assay
+0.2
-0.0
-0.9
-0.4
-0.1
stress
methylglyoxal 0.009 vol%
+0.0
-0.3
-0.8
-0.2
-0.1
stress
Benzalkonium Chloride 0.012 mg/ml
-0.0
+0.1
-0.5
-0.4
-0.3
carbon source
L-Leucine (C)
+0.1
+0.1
-0.4
-0.9
-0.1
carbon source
L-Ornithine
-0.2
+0.4
-0.7
-0.6
-0.0
carbon source
D-Glucose
+0.1
+0.3
-0.7
-0.3
-0.5
survival
4C survival; 672 hrs
-0.1
+0.1
-0.5
-0.8
+0.2
stress
Cobalt chloride 0.16 mM
-0.2
-0.2
-0.6
-0.4
+0.4
carbon source
acetate (C)
+0.3
-0.2
-0.4
-0.1
-0.6
stress
Sisomicin 0.008 mg/ml
+0.2
+0.1
-0.3
-1.0
+0.1
carbon source
L-Valine (C)
-0.2
+0.2
-0.3
-0.6
+0.1
carbon source
L-Citrulline
+0.1
-0.2
-0.9
+0.3
-0.0
carbon source
L-Ornithine
-0.1
+0.5
-0.2
-0.3
-0.3
pH
Minimal media pH9
-0.0
+0.2
-0.7
-0.5
+0.6
motility
outer cut, LB soft agar motility assay
+0.3
-0.3
-0.8
-0.2
+0.5
carbon source
acetate (C)
+0.1
+0.0
-0.4
-0.4
+0.3
carbon source
L-Citrulline
+0.2
+0.0
-0.6
-0.2
+0.2
stress
Cisplatin 0.006 mg/ml
-0.2
-0.0
-0.4
+0.3
+0.3
stress
Cisplatin 0.006 mg/ml
+0.2
+0.4
-0.9
+0.6
-0.3
carbon source
L-Ornithine
-0.2
+0.7
-0.5
-0.0
+0.1
denitrifying
Nitrate reduction, replicate B
-0.1
+0.4
+0.4
-0.0
-0.5
stress
Rifampicin 0.005 mg/ml
+0.1
-0.5
+0.4
+0.4
-0.1
stress
perchlorate 75 mM
-0.4
+0.6
-0.1
+0.2
+0.3
stress
Doxycycline hyclate 0.0002 mg/ml
+0.3
-0.2
+0.3
+0.6
+0.1
stress
Lomefloxacin 0.001 mM
+0.3
-0.4
+0.3
+1.0
+0.1
stress
nitrate 200 mM
-0.3
+0.8
-0.1
+0.5
+0.6
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