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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_13565 and AO353_13570 are separated by 149 nucleotides
AO353_13570 and AO353_13575 are separated by 89 nucleotides
AO353_13575 and AO353_13580 are separated by 54 nucleotides
AO353_13580 and AO353_13585 are separated by 47 nucleotides
AO353_13565: AO353_13565 - peptidase, at 3,011,474 to 3,012,856
_13565
AO353_13570: AO353_13570 - TonB-dependent receptor, at 3,013,006 to 3,015,135
_13570
AO353_13575: AO353_13575 - hypothetical protein, at 3,015,225 to 3,015,644
_13575
AO353_13580: AO353_13580 - copper resistance protein CopZ, at 3,015,699 to 3,016,178
_13580
AO353_13585: AO353_13585 - predicted copper homeostasis protein
(from data)
, at 3,016,226 to 3,016,627
_13585
Group
Condition
AO353
_13565
AO353
_13570
AO353
_13575
AO353
_13580
AO353
_13585
stress
Nickel (II) chloride 0.625 mM
+0.1
-0.4
-1.1
-1.8
-0.6
motility
outer cut, LB soft agar motility assay
+0.0
-0.8
+0.6
-2.1
-1.0
carbon source
L-Citrulline
+0.2
+0.0
-2.0
-0.9
-0.5
temperature
LB 30C
-0.2
-1.1
-0.5
-0.7
-0.3
temperature
LB 30C
+0.0
-0.7
-0.7
-0.8
-0.5
temperature
LB 20C
+0.1
-0.6
-0.6
-0.9
-0.6
stress
sodium fluoride 6.25 mM
+0.3
-1.1
-0.2
-1.2
-0.4
stress
acetate 68 mM
-0.4
-0.7
+0.1
-1.2
-0.2
survival
4C survival; 360 hrs
+0.3
-0.5
-0.8
-0.5
-0.7
stress
Doxycycline hyclate 0.0006 mg/ml
+0.1
+0.0
-1.1
-1.4
+0.5
carbon source
L-Ornithine
+0.4
+0.1
-0.3
-0.9
-1.1
stress
Benzalkonium Chloride 0.012 mg/ml
+0.2
+0.2
-1.4
-1.1
+0.5
carbon source
L-Ornithine
+0.2
-0.1
+0.3
-1.2
-0.6
stress
Cobalt chloride 0.32 mM
+0.2
+0.9
+0.3
-1.8
-0.6
carbon source
L-Ornithine
+0.0
+0.4
+0.7
-1.2
-0.7
carbon source
L-Citrulline
+0.5
+0.3
+0.2
-1.6
+0.1
carbon source
L-Alanine (C)
-0.0
+0.3
-1.3
+0.5
+0.5
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.7
+0.1
-0.5
+0.8
+0.6
carbon source
L-Ornithine
+0.6
+0.1
+0.5
-1.0
+0.3
nitrogen source
Inosine (N)
+0.4
+0.3
+0.7
-0.2
+0.7
carbon source
L-Valine (C)
+0.1
+0.5
+0.6
+0.4
+0.7
carbon source
propionate (C)
+0.2
+0.8
+0.3
+0.5
+0.5
stress
Cisplatin 0.006 mg/ml
+0.3
+0.5
+0.5
+0.5
+0.6
stress
Cisplatin 0.006 mg/ml
+0.3
+0.4
-0.5
+1.0
+1.2
carbon source
acetate (C)
+0.2
+1.2
-0.1
+0.6
+0.7
stress
Paraquat dichloride 0.032 mg/mL
+0.0
+1.1
+0.3
+0.5
+0.8
pH
Minimal media pH9
+0.2
+1.2
+0.8
-0.3
+1.0
pH
Minimal media pH9
-0.2
+1.5
+1.1
+0.6
+1.5
carbon source
acetate (C)
+0.5
+1.4
+1.0
+0.8
+0.9
stress
Sisomicin 0.008 mg/ml
+0.4
+2.8
+1.5
+2.2
+1.8
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