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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_13530 and AO353_13535 overlap by 4 nucleotides
AO353_13535 and AO353_13540 overlap by 1 nucleotides
AO353_13540 and AO353_13545 are separated by 132 nucleotides
AO353_13545 and AO353_13550 are separated by 364 nucleotides
AO353_13530: AO353_13530 - ABC transporter ATP-binding protein, at 3,003,291 to 3,004,160
_13530
AO353_13535: AO353_13535 - urea ABC transporter permease subunit UrtC, at 3,004,157 to 3,005,236
_13535
AO353_13540: AO353_13540 - urea ABC transporter permease, at 3,005,236 to 3,006,735
_13540
AO353_13545: AO353_13545 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,006,868 to 3,008,133
_13545
AO353_13550: AO353_13550 - ABC transporter permease, at 3,008,498 to 3,009,508
_13550
Group
Condition
AO353
_13530
AO353
_13535
AO353
_13540
AO353
_13545
AO353
_13550
nitrogen source
Parabanic Acid (N)
-4.1
-1.2
-1.9
-1.7
N.D.
nitrogen source
Inosine (N)
-0.1
-0.7
-0.6
-0.7
N.D.
pH
Minimal media pH9
-0.9
-0.3
-0.2
-0.1
N.D.
nitrogen source
Adenine (N)
-0.2
-0.5
-0.5
-0.3
N.D.
carbon source
acetate (C)
-1.0
-0.4
+0.1
-0.1
N.D.
denitrifying
Nitrate reduction, replicate B
-0.5
-0.0
-0.5
-0.3
N.D.
nitrogen source
Glycine (N)
-1.1
+0.0
-0.1
-0.0
N.D.
carbon source
L-Alanine (C)
-0.7
-0.2
-0.0
-0.2
N.D.
nitrogen source
Glycine (N)
-0.8
-0.2
-0.1
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.7
-0.1
-0.4
-0.7
N.D.
stress
Cisplatin 0.006 mg/ml
-0.7
+0.0
+0.3
+0.1
N.D.
carbon source
Ethanol (C)
-0.5
+0.2
-0.1
+0.3
N.D.
survival
4C survival; 672 hrs
-0.4
+0.4
-0.0
+0.1
N.D.
stress
Paraquat dichloride 0.032 mg/mL
+0.2
-0.6
+0.4
+0.1
N.D.
stress
Spectinomycin 0.2 mg/ml
+0.7
+0.0
-0.2
-0.1
N.D.
carbon source
D-Glucose
+0.5
-0.3
+0.1
+0.1
N.D.
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.5
+0.2
-0.2
-0.1
N.D.
carbon source
L-Citrulline
+0.9
-0.4
+0.0
+0.0
N.D.
carbon source
L-Citrulline
+0.7
-0.4
+0.0
+0.3
N.D.
carbon source
L-Leucine (C)
+0.8
-0.0
-0.0
-0.1
N.D.
carbon source
D-Alanine (C)
+0.6
-0.0
+0.2
-0.1
N.D.
stress
Lomefloxacin 0.001 mM
+0.6
+0.2
-0.2
+0.0
N.D.
carbon source
L-Tryptophan (C)
+1.0
-0.2
-0.0
-0.0
N.D.
lb
LB
+0.8
-0.1
+0.1
+0.0
N.D.
carbon source
L-Ornithine
+0.8
+0.2
-0.1
+0.0
N.D.
stress
Cisplatin 0.006 mg/ml
+0.5
+0.4
+0.1
+0.0
N.D.
nitrogen source
L-Glutamine (N)
+0.7
+0.1
+0.1
+0.2
N.D.
carbon source
L-Citrulline
+0.8
+0.3
+0.0
-0.0
N.D.
carbon source
D-Trehalose (C)
+0.6
+0.3
+0.1
+0.3
N.D.
carbon source
D-Glucose
+1.1
-0.0
+0.1
+0.3
N.D.
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