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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_13510 and AO353_13515 are separated by 9 nucleotides
AO353_13515 and AO353_13520 are separated by 142 nucleotides
AO353_13520 and AO353_13525 are separated by 31 nucleotides
AO353_13525 and AO353_13530 are separated by 143 nucleotides
AO353_13510: AO353_13510 - acetyltransferase, at 3,000,590 to 3,001,123
_13510
AO353_13515: AO353_13515 - Urease (EC 3.5.1.5)
(from data)
, at 3,001,133 to 3,001,435
_13515
AO353_13520: AO353_13520 - urease accessory protein UreD, at 3,001,578 to 3,002,417
_13520
AO353_13525: AO353_13525 - ABC transporter ATP-binding protein, at 3,002,449 to 3,003,147
_13525
AO353_13530: AO353_13530 - ABC transporter ATP-binding protein, at 3,003,291 to 3,004,160
_13530
Group
Condition
AO353
_13510
AO353
_13515
AO353
_13520
AO353
_13525
AO353
_13530
nitrogen source
Parabanic Acid (N)
-0.6
-2.4
-2.0
-2.3
-4.1
nitrogen source
Urea (N)
-0.9
-4.3
-4.2
+0.1
+0.1
nitrogen source
Inosine (N)
-0.5
-2.2
-3.1
-0.1
-0.1
nitrogen source
Adenine (N)
-0.4
-2.1
-1.8
-0.3
-0.2
motility
outer cut, LB soft agar motility assay
-1.0
-0.3
+0.0
-0.5
-0.4
denitrifying
Nitrate reduction, replicate C
-0.3
-1.1
-0.3
-0.0
-0.4
nitrogen source
Gly-Glu (N)
-0.4
-0.5
-0.6
+0.1
-0.1
stress
perchlorate 75 mM
+0.1
-0.4
-0.6
+0.1
-0.5
carbon source
acetate (C)
+0.4
-0.0
-0.4
-0.2
-1.0
motility
outer cut, LB soft agar motility assay
-0.8
-0.9
-0.4
+0.5
+0.4
stress
Paraquat dichloride 0.032 mg/mL
-0.7
-0.6
-0.0
+0.2
+0.2
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.4
-0.5
-0.0
-0.3
+0.6
stress
Spectinomycin 0.2 mg/ml
+0.2
-0.8
-0.3
-0.2
+0.7
stress
Cisplatin 0.006 mg/ml
-0.1
-0.3
+0.3
-0.9
+0.5
motility
outer cut, LB soft agar motility assay
-0.1
-1.7
+0.6
+0.1
+0.7
carbon source
L-Tryptophan (C)
-0.3
-1.3
-0.0
+0.3
+1.0
carbon source
D-Trehalose (C)
-0.2
+0.3
-1.1
+0.3
+0.6
carbon source
D-Alanine (C)
-1.0
+0.5
-0.1
-0.1
+0.6
stress
Spectinomycin 0.15 mg/ml
+0.3
-0.8
+0.5
-0.2
+0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.6
-1.0
+0.7
+0.5
+0.5
survival
4C survival; 672 hrs
+0.6
+0.5
-0.2
+0.1
-0.4
carbon source
L-Leucine (C)
+0.1
-0.3
+0.3
-0.3
+0.8
carbon source
L-Citrulline
+0.4
-0.8
-0.0
+0.5
+0.8
carbon source
L-Citrulline
+0.4
-0.3
+0.4
-0.1
+0.9
carbon source
D-Glucose
+0.2
+1.1
-0.8
+0.3
+0.5
carbon source
L-Ornithine
+0.2
+0.8
+0.2
+0.3
-0.3
carbon source
L-Citrulline
-0.5
+0.5
+0.6
+0.1
+0.7
carbon source
L-Ornithine
-0.2
+0.8
+0.1
+0.3
+0.8
lb
LB
+0.2
+0.5
+0.3
+0.0
+0.8
carbon source
D-Glucose
+0.4
-0.2
+0.5
+0.6
+1.1
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