Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_13460 and AO353_13465 are separated by 192 nucleotidesAO353_13465 and AO353_13470 are separated by 128 nucleotidesAO353_13470 and AO353_13475 are separated by 296 nucleotidesAO353_13475 and AO353_13480 are separated by 23 nucleotides AO353_13460: AO353_13460 - phosphate starvation-inducible protein PsiF, at 2,986,766 to 2,987,071 _13460 AO353_13465: AO353_13465 - hypothetical protein, at 2,987,264 to 2,988,277 _13465 AO353_13470: AO353_13470 - heat-shock protein, at 2,988,406 to 2,989,680 _13470 AO353_13475: AO353_13475 - DNA-binding protein, at 2,989,977 to 2,990,927 _13475 AO353_13480: AO353_13480 - chaperone modulatory protein CbpM, at 2,990,951 to 2,991,256 _13480
Group Condition AO353_13460 AO353_13465 AO353_13470 AO353_13475 AO353_13480
motility outer cut, LB soft agar motility assay -0.6 -0.5 +0.2 -1.2 -0.5
stress Benzalkonium Chloride 0.012 mg/ml -0.6 -1.5 -0.4 +0.2 -0.1
motility outer cut, LB soft agar motility assay -1.2 +0.3 -0.6 -0.3 -0.1
anaerobic N1B4wt with N2E3m denitrifying replicate C -0.0 -0.8 -0.5 -0.2 -0.0
stress Dimethyl Sulfoxide 7.5 vol% -1.0 +0.2 -0.5 +0.1 -0.3
stress Paraquat dichloride 0.032 mg/mL -0.7 -0.2 +0.1 -0.4 -0.2
temperature LB 30C -0.8 +0.0 +0.1 -0.4 -0.3
carbon source L-Valine (C) -0.7 +0.3 -0.4 -0.2 -0.0
survival 4C survival; 672 hrs +0.0 +0.3 -0.4 +0.4 -1.1
pH Minimal media pH9 -0.8 +0.5 -0.7 +0.2 -0.0
stress Doxycycline hyclate 0.0006 mg/ml +0.5 +0.4 -0.6 -0.3 -0.3
carbon source L-Ornithine -0.5 +0.4 -0.1 +0.4 -0.6
motility outer cut, LB soft agar motility assay +0.1 -0.1 +0.2 -0.9 +0.3
carbon source L-Citrulline +0.2 +0.3 +0.2 +0.1 -1.1
denitrifying Nitrate reduction, replicate C -0.5 -0.3 -0.1 +0.3 +0.4
carbon source L-Phenylalanine (C) -0.3 -0.5 +0.2 -0.1 +0.5
carbon source L-Ornithine -0.3 +0.5 +0.2 -0.4 -0.1
stress Cholin acetate 19 mM -0.6 +0.6 -0.5 +0.1 +0.3
carbon source Ethanol (C) +0.1 +0.7 +0.1 -0.4 -0.6
stress Benzalkonium Chloride 0.01 mg/ml +0.5 -0.9 +0.0 +0.2 +0.1
carbon source Ethanol (C) +0.4 +0.6 -0.3 -0.2 -0.1
carbon source L-Citrulline -0.0 +0.7 +0.4 +0.3 -1.0
carbon source Trisodium citrate (C) +0.5 +0.6 -0.3 -0.3 -0.0
stress Sisomicin 0.008 mg/ml -0.2 +1.0 -0.3 -0.1 +0.2
anaerobic Anaerobic, acetate donor; nitrate and iron +0.7 -0.2 -0.9 +0.1 +1.0
stress acetate 34 mM -0.5 +0.5 +0.2 +0.2 +0.4
carbon source D-Glucose -0.2 +0.7 +0.4 +0.2 -0.3
stress Cisplatin 0.006 mg/ml +0.4 -0.3 -0.1 -0.1 +0.9
carbon source D-Serine (C) +0.7 +0.4 -0.3 +0.0 +0.3
stress Doxycycline hyclate 0.0002 mg/ml +0.8 +0.3 +0.1 +0.1 +0.4
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