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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_13460 and AO353_13465 are separated by 192 nucleotides
AO353_13465 and AO353_13470 are separated by 128 nucleotides
AO353_13470 and AO353_13475 are separated by 296 nucleotides
AO353_13475 and AO353_13480 are separated by 23 nucleotides
AO353_13460: AO353_13460 - phosphate starvation-inducible protein PsiF, at 2,986,766 to 2,987,071
_13460
AO353_13465: AO353_13465 - hypothetical protein, at 2,987,264 to 2,988,277
_13465
AO353_13470: AO353_13470 - heat-shock protein, at 2,988,406 to 2,989,680
_13470
AO353_13475: AO353_13475 - DNA-binding protein, at 2,989,977 to 2,990,927
_13475
AO353_13480: AO353_13480 - chaperone modulatory protein CbpM, at 2,990,951 to 2,991,256
_13480
Group
Condition
AO353
_13460
AO353
_13465
AO353
_13470
AO353
_13475
AO353
_13480
motility
outer cut, LB soft agar motility assay
-0.6
-0.5
+0.2
-1.2
-0.5
stress
Benzalkonium Chloride 0.012 mg/ml
-0.6
-1.5
-0.4
+0.2
-0.1
motility
outer cut, LB soft agar motility assay
-1.2
+0.3
-0.6
-0.3
-0.1
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.0
-0.8
-0.5
-0.2
-0.0
stress
Dimethyl Sulfoxide 7.5 vol%
-1.0
+0.2
-0.5
+0.1
-0.3
stress
Paraquat dichloride 0.032 mg/mL
-0.7
-0.2
+0.1
-0.4
-0.2
temperature
LB 30C
-0.8
+0.0
+0.1
-0.4
-0.3
carbon source
L-Valine (C)
-0.7
+0.3
-0.4
-0.2
-0.0
survival
4C survival; 672 hrs
+0.0
+0.3
-0.4
+0.4
-1.1
pH
Minimal media pH9
-0.8
+0.5
-0.7
+0.2
-0.0
stress
Doxycycline hyclate 0.0006 mg/ml
+0.5
+0.4
-0.6
-0.3
-0.3
carbon source
L-Ornithine
-0.5
+0.4
-0.1
+0.4
-0.6
motility
outer cut, LB soft agar motility assay
+0.1
-0.1
+0.2
-0.9
+0.3
carbon source
L-Citrulline
+0.2
+0.3
+0.2
+0.1
-1.1
denitrifying
Nitrate reduction, replicate C
-0.5
-0.3
-0.1
+0.3
+0.4
carbon source
L-Phenylalanine (C)
-0.3
-0.5
+0.2
-0.1
+0.5
carbon source
L-Ornithine
-0.3
+0.5
+0.2
-0.4
-0.1
stress
Cholin acetate 19 mM
-0.6
+0.6
-0.5
+0.1
+0.3
carbon source
Ethanol (C)
+0.1
+0.7
+0.1
-0.4
-0.6
stress
Benzalkonium Chloride 0.01 mg/ml
+0.5
-0.9
+0.0
+0.2
+0.1
carbon source
Ethanol (C)
+0.4
+0.6
-0.3
-0.2
-0.1
carbon source
L-Citrulline
-0.0
+0.7
+0.4
+0.3
-1.0
carbon source
Trisodium citrate (C)
+0.5
+0.6
-0.3
-0.3
-0.0
stress
Sisomicin 0.008 mg/ml
-0.2
+1.0
-0.3
-0.1
+0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.7
-0.2
-0.9
+0.1
+1.0
stress
acetate 34 mM
-0.5
+0.5
+0.2
+0.2
+0.4
carbon source
D-Glucose
-0.2
+0.7
+0.4
+0.2
-0.3
stress
Cisplatin 0.006 mg/ml
+0.4
-0.3
-0.1
-0.1
+0.9
carbon source
D-Serine (C)
+0.7
+0.4
-0.3
+0.0
+0.3
stress
Doxycycline hyclate 0.0002 mg/ml
+0.8
+0.3
+0.1
+0.1
+0.4
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