Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_13015 and AO353_13020 overlap by 32 nucleotidesAO353_13020 and AO353_13025 are separated by 223 nucleotidesAO353_13025 and AO353_13030 overlap by 10 nucleotidesAO353_13030 and AO353_13035 are separated by 99 nucleotides AO353_13015: AO353_13015 - nitrate reductase, at 2,883,111 to 2,884,403 _13015 AO353_13020: AO353_13020 - hypothetical protein, at 2,884,372 to 2,885,118 _13020 AO353_13025: AO353_13025 - ADP-ribose pyrophosphatase, at 2,885,342 to 2,885,959 _13025 AO353_13030: AO353_13030 - cytoplasmic protein, at 2,885,950 to 2,886,402 _13030 AO353_13035: AO353_13035 - serine/threonine protein phosphatase, at 2,886,502 to 2,887,317 _13035
Group Condition AO353_13015 AO353_13020 AO353_13025 AO353_13030 AO353_13035
motility outer cut, LB soft agar motility assay -1.3 -0.2 -0.2 +0.3 N.D.
carbon source propionate (C) -0.2 -1.5 -0.4 +0.9 N.D.
pH Minimal media pH9 -0.7 +0.5 +0.8 -1.8 N.D.
carbon source L-Isoleucine (C) -0.4 -0.3 +0.3 -0.8 N.D.
carbon source L-Alanine (C) -0.5 -0.5 +0.2 -0.4 N.D.
stress Doxycycline hyclate 0.0002 mg/ml -0.2 -0.7 -0.3 +0.1 N.D.
carbon source D-Fructose (C) -0.5 -0.2 -0.5 +0.3 N.D.
nitrogen source L-Glutamine (N) -0.9 +0.0 +0.3 -0.2 N.D.
carbon source propionate (C) -0.4 -1.3 +0.0 +1.0 N.D.
carbon source D-Ribose (C) -0.1 -0.8 -0.3 +0.6 N.D.
carbon source D-Ribose (C) -0.1 -0.7 -0.5 +0.7 N.D.
stress methylglyoxal 0.009 vol% +0.6 -0.2 -0.3 -0.6 N.D.
motility outer cut, LB soft agar motility assay -0.5 +0.3 -0.7 +0.4 N.D.
carbon source L-Serine (C) +0.2 -0.3 -0.6 +0.3 N.D.
nitrogen source Gly-DL-Asp (N) -0.3 +0.3 +0.4 -0.5 N.D.
carbon source L-Citrulline (C) -0.4 +0.3 +0.5 -0.4 N.D.
nitrogen source L-Phenylalanine (N) +0.1 +0.2 +0.5 -0.5 N.D.
carbon source L-Ornithine -0.3 +0.4 +0.7 -0.3 N.D.
pH Minimal media pH9 -0.2 +0.5 +0.6 -0.2 N.D.
anaerobic Anaerobic, acetate donor; nitrate and iron +0.5 -0.4 +0.2 +0.5 N.D.
carbon source L-Ornithine -0.3 +0.3 +0.8 +0.1 N.D.
carbon source L-Citrulline -0.3 +0.3 +1.1 -0.1 N.D.
carbon source D-Glucose -0.3 +0.1 +0.7 +0.4 N.D.
carbon source L-Ornithine -0.5 +0.5 +1.2 -0.1 N.D.
anaerobic N1B4wt with N2E3m denitrifying replicate C +0.1 +0.6 +0.8 -0.5 N.D.
stress Thallium(I) acetate 0.016 mg/ml +0.7 -0.2 -0.1 +0.7 N.D.
carbon source L-Citrulline -0.2 +0.5 +0.7 +0.2 N.D.
carbon source L-Citrulline -0.2 +0.5 +0.9 +0.1 N.D.
carbon source L-Citrulline +0.2 +0.1 +0.9 +0.3 N.D.
carbon source L-Ornithine -0.1 +0.5 +1.3 +0.5 N.D.
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