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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_12760 and AO353_12765 overlap by 4 nucleotides
AO353_12765 and AO353_12770 are separated by 6 nucleotides
AO353_12770 and AO353_12775 are separated by 58 nucleotides
AO353_12775 and AO353_12780 are separated by 34 nucleotides
AO353_12760: AO353_12760 - hypothetical protein, at 2,808,142 to 2,808,438
_12760
AO353_12765: AO353_12765 - transcriptional regulator, at 2,808,435 to 2,808,836
_12765
AO353_12770: AO353_12770 - hypothetical protein, at 2,808,843 to 2,810,306
_12770
AO353_12775: AO353_12775 - potassium transporter KefA, at 2,810,365 to 2,813,712
_12775
AO353_12780: AO353_12780 - potassium:proton antiporter, at 2,813,747 to 2,815,489
_12780
Group
Condition
AO353
_12760
AO353
_12765
AO353
_12770
AO353
_12775
AO353
_12780
pH
Minimal media pH9
-0.1
-3.1
+0.2
-0.1
-0.5
stress
methylglyoxal 0.009 vol%
-0.5
-2.0
+0.0
+0.2
-0.3
stress
Cisplatin 0.006 mg/ml
-0.2
-0.8
+0.0
-0.7
-0.3
carbon source
propionate (C)
-0.5
-0.5
+0.1
-0.4
-0.6
carbon source
propionate (C)
-0.3
-0.4
+0.0
-0.5
-0.4
motility
outer cut, LB soft agar motility assay
-0.3
-0.7
-0.2
-0.0
-0.2
carbon source
acetate (C)
-0.4
+0.0
-0.2
-0.3
-0.5
stress
Fusidic 0.125 mg/ml
-0.1
-0.6
-0.0
-0.2
-0.4
carbon source
Fumarate (C)
-0.1
-0.8
-0.1
-0.3
+0.1
stress
Sisomicin 0.008 mg/ml
-0.2
-1.2
-0.3
-0.3
+1.0
stress
Benzalkonium Chloride 0.01 mg/ml
+0.1
-1.1
+0.1
-0.0
+0.0
carbon source
acetate (C)
-0.9
+0.0
+0.4
-0.3
-0.0
stress
1-ethyl-3-methylimidazolium lysine 10.5 mM
+0.3
-0.8
-0.1
-0.2
+0.1
carbon source
L-Alanine (C)
-0.4
+0.6
-0.4
-0.1
-0.2
nitrogen source
D-Glucosamine Hydrochloride (N)
+0.2
-0.9
+0.1
+0.0
+0.2
stress
Lomefloxacin 0.001 mM
-0.7
+0.2
+0.2
+0.1
-0.1
stress
Cisplatin 0.006 mg/ml
+0.4
+0.7
-0.1
-0.7
-0.4
stress
Tetracycline 0.0016 mg/ml
-0.7
+0.6
-0.2
+0.3
-0.0
stress
Doxycycline hyclate 0.0006 mg/ml
+0.5
-0.9
+0.2
-0.1
+0.3
carbon source
L-Valine (C)
+0.5
+0.3
-0.0
+0.0
-0.8
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.9
+0.7
-0.4
-0.2
-0.7
denitrifying
Nitrate reduction, replicate C
+0.5
-0.4
+0.3
+0.1
-0.1
stress
Spectinomycin 0.2 mg/ml
-0.1
+0.7
-0.1
-0.3
+0.2
carbon source
D-Trehalose (C)
-0.4
+0.7
+0.0
-0.0
+0.2
carbon source
L-Citrulline
+0.6
-0.4
+0.0
+0.1
+0.2
carbon source
L-Citrulline
+0.6
-0.7
+0.3
+0.1
+0.4
carbon source
D-Glucose
+0.1
+0.4
+0.2
+0.2
+0.5
carbon source
L-Ornithine
+0.6
-0.5
+0.4
+0.3
+0.6
carbon source
L-Ornithine
+0.0
+0.8
+0.2
+0.3
+0.9
carbon source
L-Citrulline
+0.6
+0.7
+0.1
+0.3
+0.5
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