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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_12575 and AO353_12580 overlap by 4 nucleotides
AO353_12580 and AO353_12585 overlap by 4 nucleotides
AO353_12585 and AO353_12590 overlap by 4 nucleotides
AO353_12590 and AO353_12595 are separated by 10 nucleotides
AO353_12575: AO353_12575 - type II secretion system protein GspJ, at 2,770,671 to 2,771,216
_12575
AO353_12580: AO353_12580 - type II secretory protein PulK, at 2,771,213 to 2,772,067
_12580
AO353_12585: AO353_12585 - hypothetical protein, at 2,772,064 to 2,773,161
_12585
AO353_12590: AO353_12590 - hypothetical protein, at 2,773,158 to 2,773,625
_12590
AO353_12595: AO353_12595 - hypothetical protein, at 2,773,636 to 2,774,913
_12595
Group
Condition
AO353
_12575
AO353
_12580
AO353
_12585
AO353
_12590
AO353
_12595
stress
Cisplatin 0.006 mg/ml
+0.6
-3.2
-0.1
-0.6
-0.0
stress
Nickel (II) chloride 0.625 mM
+0.3
-2.5
-0.1
-0.3
-0.4
carbon source
L-Valine (C)
-2.2
+0.1
-0.1
-0.1
-0.1
stress
methylglyoxal 0.009 vol%
+0.0
-2.9
+0.2
+0.4
+0.0
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.9
+1.3
-0.8
-1.7
-0.1
stress
Tetracycline 0.0016 mg/ml
-0.3
-0.2
-0.5
-1.0
-0.3
nitrogen source
Gly-DL-Asp (N)
-1.2
-0.4
-0.4
+0.1
+0.2
motility
outer cut, LB soft agar motility assay
-1.3
-0.8
-0.0
+1.0
+0.1
denitrifying
Nitrate reduction, replicate B
+0.4
-1.0
-0.3
+0.0
-0.1
nitrogen source
L-Threonine (N)
-0.4
-1.0
+0.6
-0.2
+0.1
stress
nitrate 200 mM
-0.8
+0.8
-0.6
-0.1
-0.1
stress
Cisplatin 0.006 mg/ml
-0.3
+1.3
-0.4
-1.1
-0.1
carbon source
D-Alanine (C)
+0.5
+0.5
-0.3
-1.1
+0.1
carbon source
D-Glucose
+0.6
-1.0
+0.3
-0.0
-0.0
stress
Benzalkonium Chloride 0.012 mg/ml
+0.3
-0.8
-0.2
+0.7
-0.1
motility
outer cut, LB soft agar motility assay
-0.6
-0.1
+0.6
-0.2
+0.5
motility
outer cut, LB soft agar motility assay
-0.6
+1.0
+0.3
-0.4
-0.1
carbon source
L-Citrulline
+0.1
+0.8
+0.4
-1.2
+0.2
stress
Doxycycline hyclate 0.0006 mg/ml
-1.4
+1.0
+0.5
+0.5
-0.1
stress
Gentamicin 0.006 mg/ml
-0.6
+1.0
+0.1
-0.1
+0.1
pH
Minimal media pH9
-0.9
+1.4
-0.4
+0.4
+0.3
carbon source
L-Citrulline
-0.6
+0.9
+0.5
-0.3
+0.3
denitrifying
Nitrate reduction, replicate C
+1.1
+0.1
+0.3
-0.3
-0.3
carbon source
L-Aspartic Acid (C)
-0.0
+1.7
-0.5
-0.3
+0.1
carbon source
L-Ornithine
-0.3
+1.1
+0.2
+0.1
+0.2
carbon source
Trisodium citrate (C)
+0.6
+1.0
-0.1
-0.2
+0.2
carbon source
acetate (C)
+0.1
+1.0
-0.2
+0.6
+0.2
stress
Paraquat dichloride 0.032 mg/mL
+0.1
+0.8
-0.2
+0.5
+0.6
carbon source
D-Ribose (C)
+0.3
+0.6
-0.0
+0.6
+0.4
carbon source
Tween 20 (C)
+0.6
+0.8
-0.1
+0.4
+0.1
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