Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_12335 and AO353_12340 are separated by 231 nucleotidesAO353_12340 and AO353_12345 overlap by 4 nucleotidesAO353_12345 and AO353_12350 overlap by 4 nucleotidesAO353_12350 and AO353_12355 are separated by 10 nucleotides AO353_12335: AO353_12335 - chemotaxis protein, at 2,715,480 to 2,717,396 _12335 AO353_12340: AO353_12340 - 16S rRNA methyltransferase, at 2,717,628 to 2,718,335 _12340 AO353_12345: AO353_12345 - twin-arginine protein translocation system subunit TatC, at 2,718,332 to 2,719,126 _12345 AO353_12350: AO353_12350 - preprotein translocase, at 2,719,123 to 2,719,569 _12350 AO353_12355: AO353_12355 - preprotein translocase subunit SecA, at 2,719,580 to 2,719,861 _12355
Group Condition AO353_12335 AO353_12340 AO353_12345 AO353_12350 AO353_12355
stress copper (II) chloride 2.5 mM +0.1 N.D. -1.7 -2.8 N.D.
stress Doxycycline hyclate 0.0006 mg/ml +0.4 N.D. -1.3 -3.1 N.D.
survival 4C survival; 672 hrs -0.1 N.D. -2.2 -1.5 N.D.
carbon source Putrescine (C) +0.2 N.D. -3.5 -0.4 N.D.
stress copper (II) chloride 2.5 mM +0.1 N.D. -1.3 -2.3 N.D.
carbon source D-Lactate (C) -0.2 N.D. -3.2 -0.2 N.D.
survival 4C survival; 360 hrs -0.2 N.D. -2.4 -0.9 N.D.
carbon source D-Serine (C) +0.0 N.D. -0.3 -3.2 N.D.
carbon source L-Ornithine -0.3 N.D. -1.9 -1.2 N.D.
pH Minimal media pH6 +0.2 N.D. -2.4 -1.0 N.D.
carbon source Carnitine Hydrochloride (C) -0.2 N.D. -2.1 -0.7 N.D.
survival 4C survival; 192 hrs +0.2 N.D. -3.1 -0.1 N.D.
nitrogen source Gly-DL-Asp (N) +0.1 N.D. -2.6 -0.3 N.D.
temperature LB 30C +0.0 N.D. -2.4 -0.3 N.D.
stress Nickel (II) chloride 0.625 mM -0.2 N.D. -1.9 -0.5 N.D.
temperature LB 30C -0.0 N.D. -2.6 +0.1 N.D.
nitrogen source Gly-Glu (N) -0.1 N.D. -1.2 -1.2 N.D.
stress syringaldehyde 5 mM +0.2 N.D. -1.6 -1.0 N.D.
carbon source L-Asparagine (C) +0.1 N.D. -2.5 -0.0 N.D.
temperature LB 20C -0.3 N.D. -2.2 +0.1 N.D.
stress Cobalt chloride 0.32 mM +0.1 N.D. -2.4 +0.2 N.D.
nitrogen source Carnitine Hydrochloride (N) +0.4 N.D. -1.3 -0.9 N.D.
motility outer cut, LB soft agar motility assay +0.4 N.D. -1.3 -0.9 N.D.
carbon source D-Ribose (C) +0.1 N.D. -2.2 +0.3 N.D.
stress Paraquat dichloride 0.032 mg/mL -0.5 N.D. +0.7 -1.4 N.D.
carbon source D-Alanine (C) +0.3 N.D. +0.8 -1.9 N.D.
carbon source L-Citrulline +0.2 N.D. -1.5 +0.9 N.D.
stress Spectinomycin 0.2 mg/ml -0.1 N.D. +1.9 -1.0 N.D.
stress Benzalkonium Chloride 0.012 mg/ml +0.2 N.D. +2.2 +1.2 N.D.
stress Sisomicin 0.008 mg/ml -0.5 N.D. +4.2 +0.9 N.D.
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