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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_12245 and AO353_12250 are separated by 528 nucleotides
AO353_12250 and AO353_12255 are separated by 151 nucleotides
AO353_12255 and AO353_12260 are separated by 170 nucleotides
AO353_12260 and AO353_12265 overlap by 4 nucleotides
AO353_12245: AO353_12245 - hypothetical protein, at 2,692,408 to 2,692,980
_12245
AO353_12250: AO353_12250 - Urocanate hydratase (EC 4.2.1.49)
(from data)
, at 2,693,509 to 2,695,212
_12250
AO353_12255: AO353_12255 - sulfonate ABC transporter substrate-binding protein, at 2,695,364 to 2,696,830
_12255
AO353_12260: AO353_12260 - ABC transporter permease, at 2,697,001 to 2,697,852
_12260
AO353_12265: AO353_12265 - hypothetical protein, at 2,697,849 to 2,698,673
_12265
Group
Condition
AO353
_12245
AO353
_12250
AO353
_12255
AO353
_12260
AO353
_12265
carbon source
L-Histidine (C)
-3.6
-3.6
-2.4
-0.7
-0.6
carbon source
L-Histidine (C)
-2.6
-3.3
-2.5
-0.9
-0.4
nitrogen source
L-Histidine (N)
-0.3
-2.7
-1.1
-0.7
-1.1
nitrogen source
L-Histidine (N)
-0.4
-2.5
-1.1
-0.5
-0.4
pH
Minimal media pH9
-0.1
-0.7
-0.6
-1.2
-1.2
carbon source
acetate (C)
-2.5
+0.0
+0.1
-1.3
+0.2
pH
Minimal media pH9
-0.4
-0.6
-0.4
-1.0
-0.9
carbon source
L-Phenylalanine (C)
-1.2
-0.6
-0.3
-0.7
-0.0
carbon source
L-Ornithine
-1.0
-0.8
-0.0
+0.0
-0.2
carbon source
L-Valine (C)
-0.6
-0.3
-0.2
-0.4
-0.2
motility
outer cut, LB soft agar motility assay
-1.1
-0.4
+0.2
-0.3
+0.4
carbon source
propionate (C)
-0.6
-0.3
+0.2
-0.5
+0.1
stress
acetate 17 mM
-1.0
+0.0
+0.1
-0.2
-0.2
stress
Chloramphenicol 0.0025 mg/ml
-0.7
+0.1
-0.1
-0.3
-0.2
carbon source
L-Ornithine
+0.3
-0.6
-0.1
-0.7
-0.0
stress
Lomefloxacin 0.001 mM
-0.1
-0.2
-0.5
-1.0
+0.5
carbon source
L-Leucine (C)
-0.2
-0.5
+0.0
-0.6
+0.4
motility
outer cut, LB soft agar motility assay
+0.3
+0.1
+0.0
-0.9
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-1.3
+0.8
+0.0
-0.4
+0.3
stress
methylglyoxal 0.009 vol%
+0.6
+0.0
+0.1
-0.6
-0.4
carbon source
L-Alanine (C)
+0.7
-0.4
-0.1
-0.3
+0.1
carbon source
D-Alanine (C)
+0.7
-0.4
+0.1
-0.4
+0.1
carbon source
D-Glucose
+0.7
-0.6
-0.1
+0.1
-0.0
carbon source
L-Citrulline
+0.6
-0.1
+0.2
-0.5
+0.2
carbon source
L-Citrulline
+0.7
-0.4
+0.0
+0.3
-0.2
carbon source
L-Asparagine (C)
-0.2
+0.1
+0.2
+0.6
+0.4
stress
Doxycycline hyclate 0.0006 mg/ml
+1.4
-0.1
-0.1
-0.3
+0.2
carbon source
L-Asparagine (C)
+0.1
+0.2
+0.1
+0.5
+0.6
nitrogen source
L-Lysine (N)
-0.4
+1.6
+0.1
+0.2
+0.4
nitrogen source
L-Lysine (N)
+0.4
+1.5
+0.1
+0.3
+0.2
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