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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_12045 and AO353_12050 overlap by 4 nucleotides
AO353_12050 and AO353_12055 overlap by 4 nucleotides
AO353_12055 and AO353_12060 are separated by 82 nucleotides
AO353_12060 and AO353_12065 are separated by 272 nucleotides
AO353_12045: AO353_12045 - iron transporter, at 2,648,129 to 2,648,401
_12045
AO353_12050: AO353_12050 - A/G-specific adenine glycosylase, at 2,648,398 to 2,649,465
_12050
AO353_12055: AO353_12055 - cell envelope biogenesis protein AsmA, at 2,649,462 to 2,651,693
_12055
AO353_12060: AO353_12060 - acetyltransferase, at 2,651,776 to 2,652,174
_12060
AO353_12065: AO353_12065 - MFS transporter, at 2,652,447 to 2,654,108
_12065
Group
Condition
AO353
_12045
AO353
_12050
AO353
_12055
AO353
_12060
AO353
_12065
carbon source
propionate (C)
-1.7
+0.0
-1.9
+0.5
-0.0
stress
Thallium(I) acetate 0.008 mg/ml
-2.1
+0.0
-0.5
-0.4
-0.1
carbon source
D-Glucose
-0.3
-0.1
-2.0
-0.3
-0.1
stress
Thallium(I) acetate 0.016 mg/ml
-1.6
-0.4
-0.5
-0.3
-0.1
carbon source
propionate (C)
-1.2
-0.1
-1.0
-0.4
-0.1
motility
outer cut, LB soft agar motility assay
-1.5
-0.2
-1.0
-0.9
+0.9
stress
Paraquat dichloride 0.032 mg/mL
-2.0
-0.1
-0.9
+0.3
-0.1
stress
Thallium(I) acetate 0.016 mg/ml
-2.0
-0.3
-0.1
-0.2
-0.1
carbon source
D-Glucose
-0.5
-0.4
-1.5
-0.3
+0.2
stress
Thallium(I) acetate 0.008 mg/ml
-1.8
-0.2
+0.1
-0.4
-0.1
carbon source
L-Ornithine
-0.7
-0.2
-1.0
-0.2
-0.1
temperature
LB 10C
-1.6
-0.1
-0.3
+0.2
+0.1
survival
4C survival; 672 hrs
-0.7
-0.2
-1.2
+0.7
-0.3
carbon source
L-Citrulline
-0.3
-0.1
-1.5
+0.7
+0.1
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-1.1
-0.2
-0.3
+0.3
+0.3
carbon source
L-Citrulline
+0.5
+0.4
-1.4
+0.2
-0.2
stress
Doxycycline hyclate 0.0002 mg/ml
+0.8
-0.1
-1.8
+0.7
-0.1
stress
acetate 17 mM
-0.7
-0.1
+0.4
+0.7
-0.3
motility
outer cut, LB soft agar motility assay
-0.5
+0.2
-1.0
+0.1
+1.1
stress
Doxycycline hyclate 0.0006 mg/ml
+1.0
-0.1
-2.1
+1.2
-0.0
stress
acetate 34 mM
-0.6
+0.0
+0.6
+0.5
-0.5
motility
outer cut, LB soft agar motility assay
-1.1
+0.0
-0.4
+0.8
+1.2
carbon source
acetate (C)
+0.4
-0.2
+0.6
+0.4
-0.6
stress
Lomefloxacin 0.001 mM
+1.0
-0.0
-0.5
+0.8
-0.1
stress
Tetracycline 0.0016 mg/ml
+1.3
-0.3
-0.8
+0.8
+0.1
stress
perchlorate 75 mM
+1.8
-0.1
-0.2
-0.2
-0.1
stress
Rifampicin 0.005 mg/ml
+1.0
-0.2
-0.4
+1.4
-0.1
carbon source
acetate (C)
+1.3
+0.1
-0.0
+0.7
-0.5
stress
Sisomicin 0.008 mg/ml
+1.4
-0.4
-0.5
+1.1
+0.3
stress
Chlorite 0.16 mM
+0.2
+0.0
-0.3
+0.8
+1.9
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