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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_11680 and AO353_11685 are separated by 194 nucleotides
AO353_11685 and AO353_11690 are separated by 342 nucleotides
AO353_11690 and AO353_11695 are separated by 16 nucleotides
AO353_11695 and AO353_11705 are separated by 865 nucleotides
AO353_11680: AO353_11680 - cysteine desulfhydrase, at 2,578,305 to 2,579,297
_11680
AO353_11685: AO353_11685 - serine acetyltransferase, at 2,579,492 to 2,580,418
_11685
AO353_11690: AO353_11690 - choline transporter, at 2,580,761 to 2,582,764
_11690
AO353_11695: AO353_11695 - hypothetical protein, at 2,582,781 to 2,583,038
_11695
AO353_11705: AO353_11705 - L-glyceraldehyde 3-phosphate reductase, at 2,583,904 to 2,584,941
_11705
Group
Condition
AO353
_11680
AO353
_11685
AO353
_11690
AO353
_11695
AO353
_11705
carbon source
L-Valine (C)
+0.2
-2.5
+0.1
+0.5
-0.0
stress
Rifampicin 0.005 mg/ml
-0.3
+0.2
-0.1
-1.4
-0.1
stress
methylglyoxal 0.009 vol%
-0.4
-0.1
-0.0
-1.2
+0.2
stress
copper (II) chloride 2.5 mM
-0.3
+0.0
-0.2
-0.9
+0.0
motility
outer cut, LB soft agar motility assay
-0.2
+0.3
-0.3
-0.4
-0.7
nitrogen source
Gly-DL-Asp (N)
+0.2
+0.1
+0.0
-1.4
-0.3
stress
Spectinomycin 0.15 mg/ml
-0.0
-0.1
+0.0
-1.0
-0.2
carbon source
L-Alanine (C)
-0.1
+0.0
+0.2
-1.1
-0.2
temperature
LB 20C
+0.0
-0.1
-0.2
-0.9
+0.1
denitrifying
Nitrate reduction, replicate C
+0.2
-0.2
-0.0
-1.0
-0.1
pH
Minimal media pH9
-0.2
+0.3
-0.1
-0.9
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
-0.0
+0.4
-0.3
-0.6
-0.3
carbon source
L-Citrulline
-0.0
-0.1
+0.4
-0.8
-0.3
stress
copper (II) chloride 2.5 mM
-0.4
+0.0
+0.1
-0.7
+0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.3
-0.1
+0.2
+0.2
-0.7
stress
Cobalt chloride 0.32 mM
+0.1
+0.1
+0.3
-0.5
-0.2
stress
Cisplatin 0.006 mg/ml
+0.6
-0.0
-0.1
-0.7
+0.1
stress
Chloride 500 mM
+0.2
+0.5
-1.2
+0.5
-0.1
stress
Thallium(I) acetate 0.016 mg/ml
-0.5
-0.6
+0.8
+0.3
+0.1
motility
outer cut, LB soft agar motility assay
-0.3
+0.1
-0.4
+0.7
+0.1
stress
Thallium(I) acetate 0.016 mg/ml
-0.5
-0.4
+0.7
+0.3
+0.1
stress
Choline chloride 358 mM
+0.1
-0.1
-0.4
+0.7
+0.1
carbon source
L-Leucine (C)
+0.1
+0.3
-0.1
+0.7
-0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.0
-0.3
+0.7
+0.4
+0.1
nitrogen source
L-Histidine (N)
-0.1
+0.3
+0.0
+0.7
+0.2
lb
LB
-0.1
-0.0
+0.1
+0.8
+0.3
carbon source
L-Phenylalanine (C)
+0.2
+0.1
+0.1
+0.9
-0.0
stress
Paraquat dichloride 0.032 mg/mL
+0.5
-0.7
+2.1
-0.2
-0.3
stress
Cisplatin 0.006 mg/ml
-0.0
+0.5
+0.2
+0.6
+0.0
carbon source
L-Citrulline
+0.1
+0.3
+0.2
+0.9
-0.0
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