Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_11530 and AO353_11535 are separated by 313 nucleotidesAO353_11535 and AO353_11540 are separated by 75 nucleotidesAO353_11540 and AO353_11545 are separated by 71 nucleotidesAO353_11545 and AO353_11550 are separated by 13 nucleotides AO353_11530: AO353_11530 - diguanylate cyclase, at 2,548,507 to 2,550,426 _11530 AO353_11535: AO353_11535 - hypothetical protein, at 2,550,740 to 2,550,922 _11535 AO353_11540: AO353_11540 - ABC transporter permease, at 2,550,998 to 2,552,011 _11540 AO353_11545: AO353_11545 - sulfate ABC transporter permease, at 2,552,083 to 2,552,901 _11545 AO353_11550: AO353_11550 - sulfate ABC transporter permease, at 2,552,915 to 2,553,787 _11550
Group Condition AO353_11530 AO353_11535 AO353_11540 AO353_11545 AO353_11550
motility outer cut, LB soft agar motility assay -2.2 N.D. -0.5 -0.7 -0.2
motility outer cut, LB soft agar motility assay -2.1 N.D. -0.5 -0.1 -0.2
carbon source L-Phenylalanine (C) -1.5 N.D. -0.1 -0.4 -0.2
motility outer cut, LB soft agar motility assay -2.0 N.D. -0.2 -0.1 +0.2
pH Minimal media pH6 -1.4 N.D. -0.4 +0.0 -0.2
temperature LB 20C -1.5 N.D. -0.1 +0.0 -0.2
carbon source Tween 20 (C) -1.0 N.D. -0.2 -0.4 -0.1
carbon source L-Lactate (C) -1.2 N.D. -0.2 -0.3 +0.1
carbon source D-Alanine (C) -1.7 N.D. -0.1 +0.3 -0.0
stress Lomefloxacin 0.001 mM -0.9 N.D. +0.1 -0.6 -0.1
carbon source L-Valine (C) -1.3 N.D. -0.3 +0.2 -0.2
carbon source propionate (C) -1.5 N.D. -0.1 +0.2 -0.0
carbon source propionate (C) -1.4 N.D. +0.1 -0.3 +0.2
stress methylglyoxal 0.009 vol% -1.5 N.D. +0.1 -0.1 +0.1
carbon source L-Ornithine -0.7 N.D. +0.3 -0.4 -0.4
stress Tetracycline 0.0008 mg/ml -1.4 N.D. +0.2 +0.1 +0.0
carbon source L-Tryptophan (C) -1.6 N.D. -0.1 +0.5 +0.3
stress Cobalt chloride 0.32 mM -0.9 N.D. -0.4 +0.5 +0.1
nitrogen source Parabanic Acid (N) -1.3 N.D. +0.0 +0.4 +0.2
stress Cisplatin 0.006 mg/ml -0.4 N.D. -0.0 +0.7 +0.5
lb LB +1.3 N.D. +0.1 +0.3 +0.1
stress acetate 68 mM +2.1 N.D. +0.6 +0.4 +0.1
stress 1-ethyl-3-methylimidazolium acetate 30 mM +2.8 N.D. +0.6 +0.6 -0.1
stress Cholin acetate 38 mM +2.9 N.D. +1.1 +0.6 +0.2
stress Cholin acetate 10 mM +3.6 N.D. +1.0 +0.6 +0.1
stress acetate 17 mM +4.4 N.D. +0.8 +0.3 +0.2
stress Cholin acetate 19 mM +4.1 N.D. +0.9 +0.6 +0.3
stress 1-ethyl-3-methylimidazolium acetate 15 mM +4.4 N.D. +1.2 +0.6 +0.1
stress acetate 34 mM +4.7 N.D. +1.0 +0.5 +0.3
stress 1-ethyl-3-methylimidazolium acetate 20 mM +4.7 N.D. +1.2 +0.7 +0.2
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