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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_10965 and AO353_10970 are separated by 76 nucleotides
AO353_10970 and AO353_10975 are separated by 7 nucleotides
AO353_10975 and AO353_10980 are separated by 10 nucleotides
AO353_10980 and AO353_10985 are separated by 443 nucleotides
AO353_10965: AO353_10965 - N-acetylmuramoyl-L-alanine amidase, at 2,393,891 to 2,394,670
_10965
AO353_10970: AO353_10970 - diguanylate phosphodiesterase, at 2,394,747 to 2,396,360
_10970
AO353_10975: AO353_10975 - histidine kinase, at 2,396,368 to 2,398,158
_10975
AO353_10980: AO353_10980 - chemotaxis protein CheY, at 2,398,169 to 2,399,515
_10980
AO353_10985: AO353_10985 - hypothetical protein, at 2,399,959 to 2,400,246
_10985
Group
Condition
AO353
_10965
AO353
_10970
AO353
_10975
AO353
_10980
AO353
_10985
stress
Doxycycline hyclate 0.0006 mg/ml
-0.1
-0.0
+0.3
-0.8
-2.8
stress
Tetracycline 0.0016 mg/ml
-0.1
-0.2
-0.3
-0.4
-1.8
carbon source
L-Citrulline
-0.4
+0.1
+0.1
-0.1
-2.0
carbon source
D-Trehalose (C)
-0.0
-0.2
-0.4
+0.0
-1.6
carbon source
L-Arginine (C)
-0.3
+0.1
-0.3
-0.1
-1.5
stress
Benzalkonium Chloride 0.012 mg/ml
+0.1
-0.1
-0.1
-0.0
-1.7
nitrogen source
Thymine (N)
-0.1
-0.1
-0.1
+0.1
-1.7
stress
Nickel (II) chloride 0.625 mM
+0.1
+0.0
-0.2
+0.1
-1.7
carbon source
D-Trehalose (C)
-0.1
-0.1
-0.3
+0.1
-1.3
stress
methylglyoxal 0.008 vol%
-0.2
+0.1
-0.0
-0.0
-1.6
stress
D-Cycloserine 0.1 mg/ml
-0.2
-0.1
-0.0
-0.4
-1.0
stress
Spectinomycin 0.15 mg/ml
+0.1
-0.0
+0.2
+0.2
-2.1
stress
Cobalt chloride 0.32 mM
-0.2
+0.0
-0.0
+0.2
-1.5
motility
outer cut, LB soft agar motility assay
+0.2
-0.1
-0.1
-0.0
-1.4
carbon source
m-Inositol (C)
-0.2
-0.2
-0.0
+0.2
-1.0
stress
nitrite 10 mM
-0.0
-0.0
+0.2
+0.1
-1.6
carbon source
L-Alanine (C)
-0.4
-0.1
-0.1
+0.2
-0.9
carbon source
D-Lactate (C)
-0.1
-0.2
-0.0
+0.2
-1.1
carbon source
L-Citrulline
-0.7
-0.2
-0.0
+0.4
-0.6
carbon source
D-Glucose
-0.1
-0.2
+0.3
+0.2
-1.4
carbon source
L-Citrulline
-0.3
-0.1
+0.4
-0.2
-1.0
carbon source
Tween 20 (C)
-0.2
+0.1
-0.3
+0.4
-1.0
motility
outer cut, LB soft agar motility assay
-0.1
+0.3
-0.4
+0.3
-1.2
carbon source
L-Citrulline
-0.4
+0.0
+0.4
+0.0
-1.1
carbon source
L-Ornithine
-0.2
+0.0
+0.2
+0.1
-1.1
nitrogen source
Parabanic Acid (N)
+0.2
-0.2
-0.1
+0.3
-1.1
carbon source
D,L-Lactate (C)
+0.2
-0.0
+0.1
+0.2
-1.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.5
+0.2
+0.1
-0.3
+0.5
denitrifying
Nitrate reduction, replicate B
+0.3
-0.0
+0.1
-0.6
+0.9
carbon source
propionate (C)
+1.4
+0.1
-0.3
+0.0
-0.7
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