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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_10895 and AO353_10900 are separated by 37 nucleotides
AO353_10900 and AO353_10905 overlap by 8 nucleotides
AO353_10905 and AO353_10910 overlap by 1 nucleotides
AO353_10910 and AO353_10915 are separated by 66 nucleotides
AO353_10895: AO353_10895 - hypothetical protein, at 2,379,726 to 2,380,439
_10895
AO353_10900: AO353_10900 - hypothetical protein, at 2,380,477 to 2,381,265
_10900
AO353_10905: AO353_10905 - zinc ABC transporter ATP-binding protein, at 2,381,258 to 2,382,043
_10905
AO353_10910: AO353_10910 - Fur family transcriptional regulator, at 2,382,043 to 2,382,525
_10910
AO353_10915: AO353_10915 - ABC transporter substrate-binding protein, at 2,382,592 to 2,383,512
_10915
Group
Condition
AO353
_10895
AO353
_10900
AO353
_10905
AO353
_10910
AO353
_10915
stress
Rifampicin 0.005 mg/ml
-1.8
-1.2
-0.8
N.D.
-1.3
motility
outer cut, LB soft agar motility assay
+0.3
-1.2
-2.1
N.D.
-1.6
carbon source
L-Arginine (C)
-0.1
-1.5
-1.4
N.D.
-1.5
carbon source
L-Arginine (C)
-0.0
-1.8
-1.3
N.D.
-1.3
carbon source
D-Lactate (C)
+0.0
-1.3
-1.4
N.D.
-1.3
carbon source
L-Phenylalanine (C)
-0.0
-1.3
-1.2
N.D.
-1.5
motility
outer cut, LB soft agar motility assay
-0.1
-1.5
-1.1
N.D.
-1.1
stress
Furfuryl Alcohol 0.25 vol%
-0.1
-1.1
-0.9
N.D.
-1.2
stress
Doxycycline hyclate 0.0006 mg/ml
-1.2
-0.7
-0.7
N.D.
-0.8
carbon source
D-Lactate (C)
+0.2
-1.3
-1.2
N.D.
-1.0
stress
methylglyoxal 0.009 vol%
-1.2
-0.5
-0.8
N.D.
-0.6
stress
Tetracycline 0.0016 mg/ml
-1.0
-0.5
-0.9
N.D.
-0.6
carbon source
acetate (C)
-0.2
-1.1
-0.6
N.D.
-1.0
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.2
-1.1
-0.9
N.D.
-0.9
stress
Doxycycline hyclate 0.0002 mg/ml
-0.3
-0.9
-0.6
N.D.
-1.0
stress
Lomefloxacin 0.001 mM
-0.0
-1.2
-0.6
N.D.
-1.0
carbon source
acetate (C)
-0.0
-1.1
-0.8
N.D.
-0.8
carbon source
pyruvate (C)
-0.5
-0.6
-0.8
N.D.
-0.8
stress
Spectinomycin 0.15 mg/ml
-1.2
-0.4
-0.4
N.D.
-0.6
pH
Minimal media pH8
-0.1
-1.1
-0.7
N.D.
-0.7
stress
Spectinomycin 0.2 mg/ml
-0.7
-0.7
-0.5
N.D.
-0.4
carbon source
D-Trehalose (C)
+0.0
-1.0
-0.5
N.D.
-0.6
carbon source
propionate (C)
+0.2
-0.9
-0.7
N.D.
-0.7
motility
outer cut, LB soft agar motility assay
+0.7
-0.6
-0.4
N.D.
-1.4
pH
Minimal media pH9
+0.8
-1.2
-0.8
N.D.
-0.4
stress
1-ethyl-3-methylimidazolium acetate 15 mM
+0.8
-0.4
-0.5
N.D.
-0.5
stress
Cholin acetate 10 mM
+0.8
-0.6
-0.2
N.D.
-0.6
carbon source
L-Ornithine
+0.5
+0.6
+0.8
N.D.
+0.6
denitrifying
Nitrate reduction, replicate C
-0.0
+1.1
+0.6
N.D.
+1.0
denitrifying
Nitrate reduction, replicate B
+0.5
+1.4
+0.6
N.D.
+1.0
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