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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_10750 and AO353_10755 are separated by 40 nucleotides
AO353_10755 and AO353_10760 are separated by 59 nucleotides
AO353_10760 and AO353_10765 are separated by 162 nucleotides
AO353_10765 and AO353_10770 are separated by 27 nucleotides
AO353_10750: AO353_10750 - hypothetical protein, at 2,352,188 to 2,352,607
_10750
AO353_10755: AO353_10755 - hypothetical protein, at 2,352,648 to 2,353,064
_10755
AO353_10760: AO353_10760 - aminopeptidase, at 2,353,124 to 2,354,200
_10760
AO353_10765: AO353_10765 - HAD family hydrolase, at 2,354,363 to 2,355,013
_10765
AO353_10770: AO353_10770 - anhydrase, at 2,355,041 to 2,355,586
_10770
Group
Condition
AO353
_10750
AO353
_10755
AO353
_10760
AO353
_10765
AO353
_10770
stress
Cobalt chloride 0.32 mM
+0.1
-0.2
+0.0
-0.3
-1.1
carbon source
L-Alanine (C)
-0.0
-0.6
-0.1
-0.7
+0.1
carbon source
L-Aspartic Acid (C)
-0.4
-0.4
-0.1
+0.1
-0.3
pH
Minimal media pH7
-0.4
+0.0
-0.1
-0.2
-0.4
stress
Doxycycline hyclate 0.0006 mg/ml
+0.4
-0.7
-0.0
-0.0
-0.8
denitrifying
Nitrate reduction, replicate C
-0.0
-0.2
-0.2
-0.6
+0.0
stress
Tetracycline 0.0016 mg/ml
-0.1
-0.2
-0.1
+0.1
-0.7
stress
copper (II) chloride 2.5 mM
-0.0
-0.2
+0.2
-0.4
-0.5
carbon source
L-Ornithine
+0.5
-1.3
-0.0
+0.2
-0.3
denitrifying
Nitrate reduction, replicate B
-0.5
-0.5
-0.1
-0.1
+0.3
nitrogen source
Gly-Glu (N)
-0.3
-0.3
+0.1
-0.5
+0.1
carbon source
L-Phenylalanine (C)
-0.4
-0.7
+0.0
+0.0
+0.2
stress
Spectinomycin 0.15 mg/ml
+0.3
-0.3
-0.1
-0.1
-0.5
carbon source
propionate (C)
-0.1
-0.3
+0.1
+0.1
-0.6
motility
outer cut, LB soft agar motility assay
+0.2
-0.5
+0.2
-0.1
-0.6
stress
Cisplatin 0.006 mg/ml
+0.1
+0.3
-0.0
-0.4
-0.6
stress
Lomefloxacin 0.001 mM
-0.5
-0.3
+0.2
+0.2
-0.0
pH
Minimal media pH9
-0.1
-0.3
+0.1
-0.5
+0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.1
+0.2
+0.1
-0.4
-0.4
carbon source
D-Glucose
-0.4
-0.2
-0.2
+0.2
+0.1
nitrogen source
Parabanic Acid (N)
-0.1
+0.1
-0.5
+0.4
-0.1
survival
4C survival; 360 hrs
+0.2
-0.5
+0.0
-0.1
+0.2
stress
Spectinomycin 0.2 mg/ml
-0.1
-0.4
-0.2
+0.1
+0.5
carbon source
L-Ornithine
+0.1
+0.4
-0.1
+0.3
-0.4
carbon source
L-Citrulline
+0.1
+0.4
-0.2
+0.1
-0.3
stress
acetate 68 mM
+0.5
-0.3
+0.0
+0.3
-0.2
stress
nitrate 200 mM
-0.0
+0.5
-0.1
-0.1
+0.4
carbon source
L-Citrulline
-0.2
+0.3
-0.2
+0.0
+0.6
carbon source
L-Serine (C)
-0.0
+0.1
-0.1
+0.0
+0.8
carbon source
L-Ornithine
+0.4
+0.5
+0.0
+0.3
-0.2
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