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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_10505 and AO353_10510 overlap by 1 nucleotides
AO353_10510 and AO353_10515 are separated by 148 nucleotides
AO353_10515 and AO353_10520 are separated by 133 nucleotides
AO353_10520 and AO353_10525 are separated by 15 nucleotides
AO353_10505: AO353_10505 - hypothetical protein, at 2,304,668 to 2,305,486
_10505
AO353_10510: AO353_10510 - benzoate transporter, at 2,305,486 to 2,306,736
_10510
AO353_10515: AO353_10515 - 3-phosphoserine/phosphohydroxythreonine aminotransferase, at 2,306,885 to 2,307,967
_10515
AO353_10520: AO353_10520 - GntR family transcriptional regulator, at 2,308,101 to 2,309,600
_10520
AO353_10525: AO353_10525 - hypothetical protein, at 2,309,616 to 2,309,810
_10525
Group
Condition
AO353
_10505
AO353
_10510
AO353
_10515
AO353
_10520
AO353
_10525
nitrogen source
L-Arginine (N)
-2.0
-1.3
-1.2
-1.1
-0.0
nitrogen source
L-Glutamine (N)
-0.5
-1.9
-1.3
-1.1
-0.0
carbon source
L-Glutamic (C)
-0.5
-2.1
-0.9
-0.9
-0.1
motility
outer cut, LB soft agar motility assay
-1.4
-0.7
+0.0
-2.5
+0.1
nitrogen source
L-Glutamic (N)
-0.4
-2.3
-0.9
-0.5
-0.2
carbon source
L-Glutamine (C)
-0.6
-1.3
-0.7
-1.0
-0.2
stress
D-Cycloserine 0.1 mg/ml
+0.0
-2.0
-0.2
-0.9
+0.1
pH
Minimal media pH6
-1.2
-0.9
-0.3
-0.6
-0.0
stress
methylglyoxal 0.009 vol%
-0.7
-0.9
-0.3
-1.0
-0.0
nitrogen source
L-Glutamine (N)
+0.2
-1.0
-0.9
-1.2
+0.0
nitrogen source
L-Glutamic (N)
-0.5
-0.3
-0.7
-1.2
-0.0
nitrogen source
Parabanic Acid (N)
-0.2
-3.1
+0.2
-0.0
+0.4
carbon source
L-Valine (C)
-0.2
-1.2
-0.2
-0.8
-0.3
carbon source
L-Glutamine (C)
-0.8
+0.0
-0.8
-0.9
-0.0
stress
Paraquat dichloride 0.032 mg/mL
-0.8
-1.7
-0.0
+0.3
-0.1
carbon source
L-Leucine (C)
-0.2
-1.9
-0.0
-0.3
+0.2
carbon source
L-Isoleucine (C)
+0.1
-2.1
+0.0
-0.3
+0.1
carbon source
L-Alanine (C)
-1.5
-0.7
-0.0
+0.2
+0.3
stress
Furfuryl Alcohol 0.0625 vol%
+0.2
-2.1
+0.0
+0.1
+0.3
carbon source
L-Glutamic (C)
-0.3
+0.6
-0.6
-1.0
-0.1
carbon source
L-Citrulline
-0.2
-1.6
+0.2
+0.5
-0.2
nitrogen source
L-Histidine (N)
-0.1
+0.6
-0.9
-0.9
+0.1
carbon source
L-Citrulline
-1.1
+1.0
-0.1
-0.0
-0.7
pH
Minimal media pH7
-0.9
+0.9
-0.4
-0.2
-0.2
stress
methylglyoxal 0.008 vol%
+0.6
-1.2
+0.1
-0.4
+0.2
motility
outer cut, LB soft agar motility assay
+0.5
+0.3
+0.1
-1.6
+0.4
motility
outer cut, LB soft agar motility assay
+0.7
-0.6
+0.5
-1.1
+0.2
survival
4C survival; 672 hrs
-1.5
+0.9
-0.3
+0.3
+0.3
stress
Cholin acetate 38 mM
+0.2
-1.2
-0.1
+1.0
-0.2
denitrifying
Nitrate reduction, replicate C
-0.3
+1.1
+0.2
+0.9
+0.1
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