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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_10145 and AO353_10150 are separated by 2 nucleotides
AO353_10150 and AO353_10155 are separated by 36 nucleotides
AO353_10155 and AO353_10160 overlap by 17 nucleotides
AO353_10160 and AO353_10165 are separated by 131 nucleotides
AO353_10145: AO353_10145 - carbonate dehydratase, at 2,219,602 to 2,220,162
_10145
AO353_10150: AO353_10150 - dihydroorotase, at 2,220,165 to 2,221,502
_10150
AO353_10155: AO353_10155 - hypothetical protein, at 2,221,539 to 2,222,075
_10155
AO353_10160: AO353_10160 - hypothetical protein, at 2,222,059 to 2,222,580
_10160
AO353_10165: AO353_10165 - cardiolipin synthase, at 2,222,712 to 2,224,151
_10165
Group
Condition
AO353
_10145
AO353
_10150
AO353
_10155
AO353
_10160
AO353
_10165
motility
outer cut, LB soft agar motility assay
-0.2
-0.8
-0.8
+0.2
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-1.2
+0.0
-0.3
-0.1
+0.1
carbon source
D-Glucose
-0.4
-0.1
-0.3
-0.3
-0.4
stress
methylglyoxal 0.009 vol%
-0.4
-0.2
-0.5
-0.3
+0.1
motility
outer cut, LB soft agar motility assay
+0.0
-0.8
+0.3
-0.5
+0.0
stress
1-ethyl-3-methylimidazolium chloride 170 mM
-0.2
-0.2
+0.1
-0.4
-0.1
stress
Cholin acetate 38 mM
-0.3
-0.4
-0.1
-0.2
+0.2
stress
copper (II) chloride 2.5 mM
+0.2
-0.4
-0.4
+0.1
-0.2
pH
Minimal media pH9
-0.2
+0.2
+0.3
-0.8
+0.1
carbon source
L-Aspartic Acid (C)
+0.1
+0.2
-0.5
-0.2
+0.1
stress
acetate 68 mM
+0.4
-0.3
+0.1
-0.4
+0.0
carbon source
L-Ornithine
+0.1
+0.1
-0.4
+0.2
-0.2
carbon source
L-Citrulline
-0.1
+0.4
+0.1
-0.4
-0.1
carbon source
L-Valine (C)
-0.6
+0.2
+0.2
+0.2
-0.1
carbon source
L-Ornithine
+0.3
+0.3
-0.4
-0.1
-0.0
nitrogen source
L-tyrosine (N)
+0.2
-0.2
-0.2
+0.3
-0.1
carbon source
L-Leucine (C)
-0.1
+0.2
-0.4
+0.3
+0.0
stress
Cholin acetate 19 mM
+0.4
-0.1
+0.1
-0.4
+0.1
stress
Benzalkonium Chloride 0.01 mg/ml
+0.2
+0.1
+0.2
+0.4
-0.5
stress
Benzethonium chloride 0.025 mM
-0.2
+0.2
+0.1
+0.4
-0.2
stress
Benzalkonium Chloride 0.012 mg/ml
+0.1
+0.0
+0.4
+0.4
-0.5
carbon source
propionate (C)
+0.2
+0.1
-0.1
+0.6
-0.3
survival
4C survival; 360 hrs
+0.4
-0.2
-0.0
+0.1
+0.3
carbon source
acetate (C)
+0.3
+0.1
+0.7
+0.1
-0.5
nitrogen source
L-Histidine (N)
+0.1
-0.2
+0.4
+0.3
-0.0
carbon source
acetate (C)
+1.1
-0.0
+0.1
-0.0
-0.4
carbon source
L-Ornithine
+0.5
-0.3
+0.0
+0.3
+0.2
stress
Doxycycline hyclate 0.0006 mg/ml
+0.4
+0.3
+0.6
-0.2
-0.1
stress
Sisomicin 0.008 mg/ml
+0.0
+0.6
+0.3
+0.3
-0.0
stress
Cisplatin 0.006 mg/ml
+0.8
+0.3
+0.5
-0.0
+0.1
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AO353_10155
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