Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_10145 and AO353_10150 are separated by 2 nucleotidesAO353_10150 and AO353_10155 are separated by 36 nucleotidesAO353_10155 and AO353_10160 overlap by 17 nucleotidesAO353_10160 and AO353_10165 are separated by 131 nucleotides AO353_10145: AO353_10145 - carbonate dehydratase, at 2,219,602 to 2,220,162 _10145 AO353_10150: AO353_10150 - dihydroorotase, at 2,220,165 to 2,221,502 _10150 AO353_10155: AO353_10155 - hypothetical protein, at 2,221,539 to 2,222,075 _10155 AO353_10160: AO353_10160 - hypothetical protein, at 2,222,059 to 2,222,580 _10160 AO353_10165: AO353_10165 - cardiolipin synthase, at 2,222,712 to 2,224,151 _10165
Group Condition AO353_10145 AO353_10150 AO353_10155 AO353_10160 AO353_10165
motility outer cut, LB soft agar motility assay -0.2 -0.8 -0.8 +0.2 -0.2
anaerobic Anaerobic, acetate donor; nitrate and iron -1.2 +0.0 -0.3 -0.1 +0.1
carbon source D-Glucose -0.4 -0.1 -0.3 -0.3 -0.4
stress methylglyoxal 0.009 vol% -0.4 -0.2 -0.5 -0.3 +0.1
motility outer cut, LB soft agar motility assay +0.0 -0.8 +0.3 -0.5 +0.0
stress 1-ethyl-3-methylimidazolium chloride 170 mM -0.2 -0.2 +0.1 -0.4 -0.1
stress Cholin acetate 38 mM -0.3 -0.4 -0.1 -0.2 +0.2
stress copper (II) chloride 2.5 mM +0.2 -0.4 -0.4 +0.1 -0.2
pH Minimal media pH9 -0.2 +0.2 +0.3 -0.8 +0.1
carbon source L-Aspartic Acid (C) +0.1 +0.2 -0.5 -0.2 +0.1
stress acetate 68 mM +0.4 -0.3 +0.1 -0.4 +0.0
carbon source L-Ornithine +0.1 +0.1 -0.4 +0.2 -0.2
carbon source L-Citrulline -0.1 +0.4 +0.1 -0.4 -0.1
carbon source L-Valine (C) -0.6 +0.2 +0.2 +0.2 -0.1
carbon source L-Ornithine +0.3 +0.3 -0.4 -0.1 -0.0
nitrogen source L-tyrosine (N) +0.2 -0.2 -0.2 +0.3 -0.1
carbon source L-Leucine (C) -0.1 +0.2 -0.4 +0.3 +0.0
stress Cholin acetate 19 mM +0.4 -0.1 +0.1 -0.4 +0.1
stress Benzalkonium Chloride 0.01 mg/ml +0.2 +0.1 +0.2 +0.4 -0.5
stress Benzethonium chloride 0.025 mM -0.2 +0.2 +0.1 +0.4 -0.2
stress Benzalkonium Chloride 0.012 mg/ml +0.1 +0.0 +0.4 +0.4 -0.5
carbon source propionate (C) +0.2 +0.1 -0.1 +0.6 -0.3
survival 4C survival; 360 hrs +0.4 -0.2 -0.0 +0.1 +0.3
carbon source acetate (C) +0.3 +0.1 +0.7 +0.1 -0.5
nitrogen source L-Histidine (N) +0.1 -0.2 +0.4 +0.3 -0.0
carbon source acetate (C) +1.1 -0.0 +0.1 -0.0 -0.4
carbon source L-Ornithine +0.5 -0.3 +0.0 +0.3 +0.2
stress Doxycycline hyclate 0.0006 mg/ml +0.4 +0.3 +0.6 -0.2 -0.1
stress Sisomicin 0.008 mg/ml +0.0 +0.6 +0.3 +0.3 -0.0
stress Cisplatin 0.006 mg/ml +0.8 +0.3 +0.5 -0.0 +0.1
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