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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_10050 and AO353_10055 are separated by 261 nucleotides
AO353_10055 and AO353_10060 are separated by 62 nucleotides
AO353_10060 and AO353_10065 are separated by 178 nucleotides
AO353_10065 and AO353_10070 are separated by 271 nucleotides
AO353_10050: AO353_10050 - diguanylate cyclase, at 2,199,590 to 2,202,457
_10050
AO353_10055: AO353_10055 - DNA helicase II, at 2,202,719 to 2,204,902
_10055
AO353_10060: AO353_10060 - transporter, at 2,204,965 to 2,205,405
_10060
AO353_10065: AO353_10065 - hypothetical protein, at 2,205,584 to 2,206,456
_10065
AO353_10070: AO353_10070 - cell wall assembly protein, at 2,206,728 to 2,207,135
_10070
Group
Condition
AO353
_10050
AO353
_10055
AO353
_10060
AO353
_10065
AO353
_10070
survival
4C survival; 672 hrs
-0.5
-1.3
+0.0
-0.5
-2.0
stress
methylglyoxal 0.008 vol%
-0.1
-3.2
-0.1
+0.2
-0.1
stress
Spectinomycin 0.2 mg/ml
+0.5
-1.2
+0.2
-2.5
-0.3
stress
Spectinomycin 0.15 mg/ml
-0.0
-1.0
+0.4
-2.2
-0.3
stress
Fusidic 0.125 mg/ml
+0.2
-2.1
-0.1
-0.8
-0.3
motility
outer cut, LB soft agar motility assay
+0.1
-2.1
-0.6
-0.3
-0.1
stress
Lomefloxacin 0.001 mM
+0.0
-2.9
+0.1
+0.1
-0.3
motility
outer cut, LB soft agar motility assay
+0.1
-2.6
+0.0
-0.3
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
-0.8
-0.5
-0.1
-0.1
-1.3
stress
copper (II) chloride 2.5 mM
+0.0
-1.0
+0.1
-1.7
-0.1
pH
Minimal media pH9
+0.0
-1.6
+0.4
-0.8
-0.7
carbon source
D-Glucose
+0.0
-1.3
+0.1
-0.5
-1.0
stress
Gentamicin 0.006 mg/ml
-0.0
-1.3
+0.8
-2.3
+0.2
stress
Furfuryl Alcohol 0.25 vol%
-0.3
-1.9
+0.2
-0.2
-0.4
carbon source
m-Inositol (C)
-0.3
-0.7
-0.2
-0.6
-0.8
carbon source
D-Serine (C)
-0.4
-1.5
-0.3
+0.0
-0.4
carbon source
propionate (C)
-0.4
-0.9
-0.1
-1.2
+0.2
carbon source
propionate (C)
-0.6
-1.0
+0.2
-1.2
+0.4
pH
Minimal media pH9
+0.2
-1.0
+0.2
-1.3
-0.1
carbon source
L-Citrulline
-0.5
-1.0
+0.1
-0.7
+0.4
stress
Vancomycin 1 mg/ml
+0.1
-1.6
+0.3
-0.2
-0.3
motility
outer cut, LB soft agar motility assay
+0.4
-2.1
-0.4
+0.2
+0.2
stress
methylglyoxal 0.009 vol%
+0.3
-2.3
+0.4
+0.3
-0.3
stress
nitrate 200 mM
+0.2
-1.9
-0.0
-0.1
+0.5
stress
Rifampicin 0.005 mg/ml
+0.3
-0.6
+0.5
-0.4
-1.1
carbon source
acetate (C)
-0.2
+0.5
+0.4
-1.6
-0.2
carbon source
acetate (C)
-0.1
-0.0
+0.8
-1.7
+0.2
stress
Cisplatin 0.006 mg/ml
+0.3
-1.8
+0.7
-0.6
+0.7
stress
Cisplatin 0.006 mg/ml
+0.2
-2.1
+0.7
-0.5
+1.0
stress
Sisomicin 0.008 mg/ml
+0.1
-1.3
+2.5
-1.7
+0.6
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