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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_09800 and AO353_09805 are separated by 3 nucleotides
AO353_09805 and AO353_09810 are separated by 105 nucleotides
AO353_09810 and AO353_09815 are separated by 75 nucleotides
AO353_09815 and AO353_09820 are separated by 67 nucleotides
AO353_09800: AO353_09800 - hypothetical protein, at 2,144,731 to 2,144,952
_09800
AO353_09805: AO353_09805 - hypothetical protein, at 2,144,956 to 2,145,243
_09805
AO353_09810: AO353_09810 - hypothetical protein, at 2,145,349 to 2,145,795
_09810
AO353_09815: AO353_09815 - hypothetical protein, at 2,145,871 to 2,146,305
_09815
AO353_09820: AO353_09820 - hypothetical protein, at 2,146,373 to 2,146,852
_09820
Group
Condition
AO353
_09800
AO353
_09805
AO353
_09810
AO353
_09815
AO353
_09820
pH
Minimal media pH9
N.D.
N.D.
-0.3
-0.2
-0.6
carbon source
acetate (C)
N.D.
N.D.
-0.7
-0.4
+0.2
stress
Benzalkonium Chloride 0.012 mg/ml
N.D.
N.D.
-0.5
-0.3
-0.2
carbon source
propionate (C)
N.D.
N.D.
-0.6
-0.4
+0.0
carbon source
propionate (C)
N.D.
N.D.
-0.8
-0.0
-0.1
nitrogen source
Gly-DL-Asp (N)
N.D.
N.D.
-0.6
-0.3
+0.2
stress
A22 0.002 mg/ml
N.D.
N.D.
-0.6
+0.3
-0.3
carbon source
D,L-Lactate (C)
N.D.
N.D.
+0.1
-0.6
-0.2
nitrogen source
L-Histidine (N)
N.D.
N.D.
-0.7
+0.2
-0.1
carbon source
L-Alanine (C)
N.D.
N.D.
-1.0
+0.4
-0.0
carbon source
L-Lactate (C)
N.D.
N.D.
-0.5
-0.3
+0.2
stress
Lomefloxacin 0.001 mM
N.D.
N.D.
-0.6
+0.5
-0.5
stress
Thallium(I) acetate 0.008 mg/ml
N.D.
N.D.
-0.2
-0.4
+0.2
nitrogen source
L-Glutamine (N)
N.D.
N.D.
-0.5
+0.4
-0.4
pH
Minimal media pH7
N.D.
N.D.
-0.4
+0.2
-0.2
stress
Nickel (II) chloride 0.625 mM
N.D.
N.D.
-0.4
+0.4
-0.4
carbon source
Trisodium citrate (C)
N.D.
N.D.
-0.3
-0.3
+0.3
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
-0.4
-0.3
+0.5
stress
acetate 68 mM
N.D.
N.D.
+0.5
-0.3
-0.2
carbon source
L-Leucine (C)
N.D.
N.D.
-0.5
+0.2
+0.3
stress
Fusidic 0.125 mg/ml
N.D.
N.D.
+0.7
-0.3
-0.2
carbon source
L-Serine (C)
N.D.
N.D.
+0.6
-0.1
-0.2
stress
Doxycycline hyclate 0.0006 mg/ml
N.D.
N.D.
+0.5
+0.2
-0.4
temperature
LB 30C
N.D.
N.D.
+0.5
+0.1
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
N.D.
N.D.
+0.3
+0.5
-0.2
nitrogen source
Carnitine Hydrochloride (N)
N.D.
N.D.
+0.2
+0.5
+0.3
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
+0.7
+0.3
-0.1
stress
Sisomicin 0.008 mg/ml
N.D.
N.D.
-0.1
+0.6
+0.3
carbon source
L-Citrulline
N.D.
N.D.
+0.4
+0.8
-0.1
carbon source
L-Citrulline
N.D.
N.D.
+0.8
+0.1
+0.3
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