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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_09615 and AO353_09620 overlap by 8 nucleotides
AO353_09620 and AO353_09625 are separated by 108 nucleotides
AO353_09625 and AO353_09630 are separated by 48 nucleotides
AO353_09630 and AO353_09635 overlap by 4 nucleotides
AO353_09615: AO353_09615 - type VI secretion protein, at 2,108,776 to 2,109,828
_09615
AO353_09620: AO353_09620 - ClpV1 family T6SS ATPase, at 2,109,821 to 2,112,499
_09620
AO353_09625: AO353_09625 - type VI secretion protein ImpA, at 2,112,608 to 2,114,548
_09625
AO353_09630: AO353_09630 - hypothetical protein, at 2,114,597 to 2,114,860
_09630
AO353_09635: AO353_09635 - hypothetical protein, at 2,114,857 to 2,115,189
_09635
Group
Condition
AO353
_09615
AO353
_09620
AO353
_09625
AO353
_09630
AO353
_09635
carbon source
L-Valine (C)
-0.3
-0.4
-0.5
+0.0
+0.0
carbon source
L-Aspartic Acid (C)
-0.2
-0.2
-0.4
-0.2
+0.0
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.1
-0.4
-0.3
-0.1
-0.0
carbon source
L-Ornithine
+0.3
-0.0
-0.8
-0.1
-0.2
survival
4C survival; 360 hrs
+0.3
-0.3
-0.6
-0.1
-0.1
carbon source
L-Ornithine
-0.3
+0.2
+0.1
-0.3
-0.2
carbon source
D-Serine (C)
-0.1
-0.1
-0.4
-0.2
+0.3
stress
Tetracycline 0.0016 mg/ml
-0.1
-0.4
-0.2
+0.0
+0.3
stress
Benzalkonium Chloride 0.01 mg/ml
+0.2
-0.2
-0.5
+0.1
+0.1
stress
Furfuryl Alcohol 0.0625 vol%
+0.2
-0.2
-0.2
+0.2
-0.1
carbon source
L-Isoleucine (C)
-0.3
-0.3
+0.0
+0.2
+0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.4
-0.3
+0.2
-0.3
-0.2
stress
Sisomicin 0.008 mg/ml
-0.2
-0.4
+0.0
+0.1
+0.3
carbon source
L-Phenylalanine (C)
-0.2
+0.2
-0.4
+0.1
+0.2
carbon source
Tween 20 (C)
+0.2
-0.1
-0.3
-0.1
+0.2
stress
Cobalt chloride 0.32 mM
-0.3
-0.3
+0.3
+0.2
+0.1
motility
outer cut, LB soft agar motility assay
-0.0
-0.2
-0.2
+0.3
+0.1
carbon source
L-Alanine (C)
-0.2
+0.1
-0.2
+0.2
+0.2
carbon source
L-Citrulline
+0.2
-0.0
+0.3
-0.0
-0.3
motility
outer cut, LB soft agar motility assay
-0.1
-0.1
+0.6
-0.1
+0.0
nitrogen source
L-Lysine (N)
+0.2
+0.2
-0.3
+0.0
+0.2
stress
Cisplatin 0.006 mg/ml
+0.3
+0.1
+0.2
-0.4
+0.1
carbon source
D,L-Lactate (C)
-0.2
+0.1
+0.4
+0.1
+0.1
carbon source
D-Alanine (C)
-0.2
+0.3
+0.3
-0.0
+0.1
carbon source
L-Lactate (C)
-0.3
+0.1
+0.4
+0.0
+0.2
stress
Nickel (II) chloride 0.625 mM
-0.2
+0.1
+0.4
+0.1
+0.1
stress
Doxycycline hyclate 0.0006 mg/ml
-0.0
+0.4
+0.3
-0.2
+0.2
carbon source
L-Citrulline
+0.3
+0.2
+0.0
+0.4
-0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.1
+0.2
+0.5
+0.1
+0.2
stress
Polymyxin B sulfate 4 mg/ml
+0.7
+0.3
+0.1
+0.0
+0.1
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