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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_09320 and AO353_09325 are separated by 78 nucleotides
AO353_09325 and AO353_09330 are separated by 246 nucleotides
AO353_09330 and AO353_09335 are separated by 254 nucleotides
AO353_09335 and AO353_09340 are separated by 19 nucleotides
AO353_09320: AO353_09320 - ATP-dependent DNA helicase RecG, at 2,042,244 to 2,044,319
_09320
AO353_09325: AO353_09325 - histidine kinase, at 2,044,398 to 2,045,798
_09325
AO353_09330: AO353_09330 - helicase, at 2,046,045 to 2,046,428
_09330
AO353_09335: AO353_09335 - transposase, at 2,046,683 to 2,048,113
_09335
AO353_09340: AO353_09340 - hypothetical protein, at 2,048,133 to 2,048,384
_09340
Group
Condition
AO353
_09320
AO353
_09325
AO353
_09330
AO353
_09335
AO353
_09340
pH
Minimal media pH9
-2.2
+0.0
-0.5
N.D.
-0.6
stress
Lomefloxacin 0.001 mM
-3.3
+0.2
+0.0
N.D.
-0.1
stress
methylglyoxal 0.009 vol%
-2.9
-0.2
-0.2
N.D.
+0.2
radiation
Ionizing radiation, 126.196 Gy,
-2.6
+0.0
-0.4
N.D.
-0.0
stress
methylglyoxal 0.008 vol%
-3.1
+0.1
+0.4
N.D.
-0.3
stress
Chloride 500 mM
-2.0
-0.0
-0.2
N.D.
-0.6
stress
Spectinomycin 0.2 mg/ml
-2.0
+0.1
-0.5
N.D.
-0.4
stress
Fusidic 0.125 mg/ml
-2.6
-0.1
+0.1
N.D.
-0.1
stress
Choline chloride 358 mM
-1.9
+0.1
-0.5
N.D.
-0.1
carbon source
L-Citrulline
-1.3
-0.6
-0.2
N.D.
-0.4
stress
Spectinomycin 0.15 mg/ml
-2.2
+0.1
-0.3
N.D.
-0.1
stress
1-ethyl-3-methylimidazolium chloride 170 mM
-1.9
-0.1
-0.2
N.D.
-0.2
stress
Benzalkonium Chloride 0.01 mg/ml
-2.2
+0.1
-0.4
N.D.
+0.1
carbon source
L-Ornithine
-1.3
+0.0
+0.0
N.D.
-1.1
stress
Benzalkonium Chloride 0.012 mg/ml
-2.0
+0.1
+0.1
N.D.
-0.5
stress
Benzethonium chloride 0.025 mM
-1.8
+0.2
-0.4
N.D.
-0.2
nitrogen source
Carnitine Hydrochloride (N)
-1.5
-0.2
-0.6
N.D.
+0.3
carbon source
L-Ornithine
-2.0
-0.1
+0.5
N.D.
-0.3
carbon source
L-Citrulline
-1.4
-0.3
+0.4
N.D.
-0.6
stress
Cisplatin 0.006 mg/ml
-2.1
+0.3
+0.4
N.D.
-0.3
stress
D-Cycloserine 0.1 mg/ml
-2.8
+0.0
+0.5
N.D.
+0.6
motility
outer cut, LB soft agar motility assay
-2.0
+2.1
-0.6
N.D.
-1.1
stress
Doxycycline hyclate 0.0002 mg/ml
-1.5
+0.2
+0.2
N.D.
-0.5
stress
Cisplatin 0.006 mg/ml
-2.0
-0.1
+0.1
N.D.
+0.5
stress
Tetracycline 0.0016 mg/ml
-1.9
+0.2
+0.1
N.D.
+0.2
pH
Minimal media pH9
-1.5
-0.2
-0.2
N.D.
+0.9
stress
Furfuryl Alcohol 0.0625 vol%
-1.7
-0.2
+0.3
N.D.
+0.6
motility
outer cut, LB soft agar motility assay
-2.0
+1.6
+0.3
N.D.
-0.7
survival
4C survival; 672 hrs
-1.2
-0.3
+0.4
N.D.
+0.8
motility
outer cut, LB soft agar motility assay
-2.6
+2.3
+0.4
N.D.
+0.5
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